Altas concentrações de FSH-p na maturação in vitro de oócitos Bos indicus

Alves, Joana D'Arc Rocha; Oliveira, Marcos Antônio Lemos; Lima, Paulo Ricardo Silva; Caldas, João Gustavo Luz; Filho, A. S. Santos; Barreto, Márcia Brayner Paes

doi:10.1590/s0103-84782001000400014

Cited by 4 publications

(2 citation statements)

References 17 publications

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“…Similarly, Jain et al (2016) not observed difference in the percentage of COCs showing metaphase II after IVM with 1, 5 and 10 μg/mL of pFSH, using the Hoechst dye. Previously, supraphysiological concentrations higher than pFSH (10 μg/mL, 20 μg/ mL and 40 μg/mL) were evaluated and a negative effect was observed (10: 81.8%, 20: 55.5%, 40: 50.0%) of greater amounts of the hormone, which may have slowed or inhibited bovine oocytes maturation, decreasing the rate of metaphase II at the end of IVM; nevertheless, the authors verified no differences in cleavage and blastocyst rates (ALVES et al, 2001). In general, the concentration of 10 μg/mL does not have deleterious effects and so it is widely used for pFSH, justifying similar maturation rates in this study.…”

Section: Discussionmentioning

confidence: 97%

“…Same after purification, urinary FSH or derived from pituitary displays a low degree of purity, with varying amounts of other hormones related to fertility, primarily LH (HOOMANS et al, 1999;LISPI et al, 2006). When it comes to origin FSH for IVM of bovine oocytes, the most used are of porcine origin with different proportions of FSH:LH (ALVES et al, 2001;CALDER et al, 2003;KAKKASSERY et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Influence of commercially available follicle stimulating hormone on the in vitro maturation of bovine oocytes

Santos

Neta

Borges

et al. 2017

SCA

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The work aimed (Experiment I) to compare commercial representations of porcine follicle stimulating hormone (FSH, Pluset® vs. Folltropin®) in concentration (10 μg/mL) and time (24 h) standard (more used in protocols of in vitro maturation, IVM); (Experiment II) to evaluate the best incubation time (6 h vs. 16 h vs. 24 h) and, (Experiment III) to analyze varying concentrations (1.0 μg/mL vs. 2.5 μg/mL vs. 10.0 μg/mL) of representations of FSH on the IVM of bovine oocytes. Thus, oocytes were recovered and submitted to IVM under appropriate conditions. After the IVM, oocytes were evaluated for expansion of cumulus cells (CCs), presence of the first polar body (1PB) and metaphase plate (MII). All the data were analyzed by the Fisher exact test (P < 0.05). Initially, in Experiment I, no difference (P > 0.05) was observed in maturation rates of the oocytes incubated with FSH Pluset® or Folltropin®, assessed by expansion of CCs (97.6% vs. 94.3%), presence of 1PB (76.6% vs. 69.4%) and MII (70.0% vs. 68.6%). In Experiment II, when the incubation time with FSH was evaluated, both Pluset® as Folltropin® showed lower rate of expansion of CCs when they were present only in the first 6 h of IVM. As for presence of 1PB, differences were observed in relation to Pluset® while Folltropin® showed similar results in all incubation times. Regarding the MII, no difference was observed between the incubation times with FSH Pluset® and Folltropin®. In Experiment III, no difference was observed in the expansion of CCs, presence of 1PB and MII for concentrations evaluated FSH Pluset® and Folltropin®. Therefore, the FSH Pluset® and Folltropin® have the same efficiency in IVM of bovine oocytes. Regarding the incubation time, it is recommended to maintain FSH (Pluset® or Folltropin®) throughout the period of IVM, since there was no difference in the results of presence of MII. Furthermore, the concentration of 1.0 μg/mL of FSH Pluset® and Folltropin® is as effective as 10 μg/mL and can therefore be used for IVM of oocytes. Key words: Cattle. Gonadotropin. In vitro production of embryos. Oocytes. ResumoO trabalho objetivou (Experimento I) comparar representações comerciais do hormônio folículo estimulante suíno (FSH, Pluset® vs. Folltropin®) na concentração (10 μg/mL) e tempo (24 h) padrão (mais utilizados nos protocolos de maturação in vitro, MIV); (Experimento II) avaliar o melhor tempo de incubação (6 h vs. 16 h vs. 24 h) e, (Experimento III) analisar concentrações variáveis (1,0 μg/mL vs. 2,5 μg/mL vs. 10,0 μg/mL) das representações de FSH sobre a MIV de oócitos bovinos. Para tanto, oócitos foram recuperados e submetidos a MIV em condições adequadas. Após a MIV, oócitos foram avaliados quanto à expansão das células do cumulus (CCs), presença do primeiro corpúsculo polar (1CP) e placa metafásica (MII). Todos os dados foram analisados pelo teste exato de Fisher (P < 0,05).

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Influence of commercially available follicle stimulating hormone on the in vitro maturation of bovine oocytes

Santos

Neta

Borges

et al. 2017

SCA

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Effects of retinoic acid on maturation of immature mouse oocytes in the presence and absence of a granulosa cell co-culture system

Tahaei

Eimani

Eftekhari‐Yazdi

et al. 2011

J Assist Reprod Genet

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Purpose Evaluation of the all-trans retinoic acid (t-RA) effects on in vitro maturation (IVM) and in vitro fertilization (IVF) of immature mouse oocytes in the presence and absence of granulosa cell monolayer. Methods Denuded oocytes isolated from mice ovaries and matured in IVM medium alone (Control I), IVM medium in the presence of granulosa cells (Control II), IVM medium with t-RA (Experimental I) and IVM medium simultaneously with t-RA and granulosa cells (Experimental II). After 24 h, matured oocytes were fertilized in T6 medium and their development was followed until the blastocyst stage. Metaphase II oocytes ploidy were evaluated by chromosome counting. Results The t-RA group compared to the control groups showed no obvious abnormalities. Additionally maturation and embryo development rates significantly increased in the t-RA treated granulosa cell co-culture system. Conclusions In conclusion, association of t-RA with granulosa cell co-culture during in vitro maturation increases meiosis resumption, formation of metaphase II oocytes, as well as 2-cell and blastocyst stage embryos.

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Effect of retinoids and growth factor on in vitro bovine embryos produced under chemically defined conditions

Lima

Oliveira

Santos

et al. 2006

Animal Reproduction Science

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Altas concentrações de FSH-p na maturação in vitro de oócitos Bos indicus

Cited by 4 publications

References 17 publications

Influence of commercially available follicle stimulating hormone on the in vitro maturation of bovine oocytes

Influence of commercially available follicle stimulating hormone on the in vitro maturation of bovine oocytes

Effects of retinoic acid on maturation of immature mouse oocytes in the presence and absence of a granulosa cell co-culture system

Effect of retinoids and growth factor on in vitro bovine embryos produced under chemically defined conditions

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