Alterações inflamatórias provocadas pelo metronidazol em feridas: estudo experimental em ratos

Sampaio, Cláudia Paraguaçu Pupo; Biondo-Simões, Maria de Lourdes Pessole; Trindade, Lilian Cristine Teixeira; Farias, Rogério Estevam; Pierin, Rodrigo Jardim; Martins, Rafael César

doi:10.1590/s1677-54492009000300008

Cited by 8 publications

(9 citation statements)

References 16 publications

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“…During the formation of granulation tissue fibroblasts and endothelial cells proliferate and move into the wound, producing extracellular matrix, connective tissue fibers and neovascularization 4,5 .Many fibroblasts acquire some morphological and biochemical properties of smooth muscle cells, giving rise to myofibroblasts. This factor participate in extracellular matrix synthesis and production of mechanical power, affecting the reorganization of the matrix and wound contraction.…”

Section: Introductionmentioning

confidence: 99%

Wound healing using ionic silver dressing and nonocrystalline silver dressing in rats

et al. 2012

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PURPOSE: To investigate the results of the healing process on surgical wounds in the back of Wistar rats using nanocristaline and ionic silver dressing. METHODS: Sixty rats Wistar were submitted to surgical wounds with punch of 8mm in diameter. In 30 animals (groups PN - nanocristaline and AD - control) two surgical wounds were done diametrically opposite on the upper back side. On the right side was used nanocristaline (PN) silver dressing and on the left side, distilled water dressing (AD). On the other group of 30 rats, only one wound was made with the punch, on the right side, and was used ionic silver dressing. So, the groups were divided into three subgroups, according to the day of death (7th, 14th and 21st day). In each of these days the wounds diameter were measured to evaluate the wound contraction. Microscopic data were analyzed using the H&E staining to verify the inflammatory process and neovascularization. The Masson trichrome staining was used to verify the fibrosis. RESULTS: Macroscopically only the subgroup of 21st day showed statistical significance; between the groups AD and PI inflammatory process appeared in the 7th day subgroup in 90% of the cases. In neovascularization there was statistical significance between the groups PN and AD in the subgroup of 7th day. Fibrosis did not show statistical significance in the studied groups. CONCLUSIONS: In relation to wound contraction, PN and PI groups showed better results than the AD group. In regard to histological analysis, H&E staining showed that there was presence of inflammation in all groups, and at the end, the control group (AD) on 7th day, was superior to PN and PI groups. In relationship to fibrosis, no differences were obtained among groups.

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Section: Introductionmentioning

confidence: 99%

Wound healing using ionic silver dressing and nonocrystalline silver dressing in rats

et al. 2012

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“…The wide variation of metronidazole concentrations for topical use at the experimental level is an additional difficulty. There are studies that report the effects of metronidazole on healing using 20mg/kg 16 , 50mg/kg 14 , 108 mg/kg 10 , 160mg/kg 13 and 180mg/kg 12 .…”

Section: Discussionmentioning

confidence: 99%

“…Evaluations were made of blood samples at different times (third, seventh and fourteenth day). It is important to determine whether topical metronidazole, capable of facilitating healing 10 , 12 , 14 , 16 , can be absorbed in the area of the lesion and maintain concentration in the blood.…”

Section: Discussionmentioning

confidence: 99%

Metronidazole concentration in the bloodstream following its topical application, at different concentration levels, on experimental skin wounds during healing by secondary intention

et al. 2019

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Purpose To characterize qualitatively and quantitatively the absorption of metronidazole solution, in greater concentrations and for longer periods, when applied topically to an experimental open skin wound model. Methods An open skin wound, 2 cm in diameter and total skin thickness was prepared, under anesthetic, in the dorsal region of 108 Wistar rats weighing between 300 and 350 grams. The animals were allocated to groups of 18 animals in accordance with the concentration of metronidazole in the solution to be applied daily to the wound. In the control group (CG), 0.9% sodium chloride solution was used for application, and in the experimental groups (GI, GII, GIII, GIV and GV) metronidazole solution at 4%, 6%, 8%, 10% and 12%, respectively, was applied. After 3, 7 and 14 days of treatment. Blood samples collected through cardiac puncture were examined for the existence or non-existence of metronidazole, using high performance liquid chromatography (HPLC). Detected metronidazole values were compared statistically within each group (temporal analysis 3 days X 7 days X 14 days) and between the groups that used topical metronidazole (4% X 6% X 8% X 10% and 12%) using the Kruskal-Wallis test, considering a statistical significance of 95% (p<0.05). Results Metronidazole was detected in all the samples at all times in all the groups in which topical metronidazole was applied to the wounds. Characteristically, there was no significant difference between the doses obtained within each group over time (3 days X 7 days X 14 days) GI=0.461; GII=0.154; GIII=0.888; GIV= 0.264 and GV=0.152. In the evaluation between groups, a similar degree of absorption was found after 3 days (p=0.829) and 14 days (p=0.751). Conclusion The serum concentration of metronidazole that was achieved was not influenced by the concentration of the solution applied to the skin wound, with similar extend, or by the duration of the application.

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“…Healing properties have been attributed to metronidazole [14, 21,32]. These properties arise from metronidazole's ability to promote contraction of myofibroblasts, stimulate the proliferation of epithelial cells, inhibit local inflammation, and reduce bacterial load [14,20,21,32].…”

Section: Discussionmentioning

confidence: 99%

“…Metronidazole also appears to have chemotherapeutic activity [18] and antioxidant effects [14]. More recently, there has been some evidence that metronidazole can control inflammation [19,20] and modulate angiogenesis in non-ocular tissues [21,22]. The aim of this study was to evaluate the angiogenic effect of metronidazole ophthalmic solutions on CNV in a rat model.…”

Section: Introductionmentioning

confidence: 99%

Effect of metronidazole ophthalmic solution on corneal neovascularization in a rat model

et al. 2018

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Alterações inflamatórias provocadas pelo metronidazol em feridas: estudo experimental em ratos

Cited by 8 publications

References 16 publications

Wound healing using ionic silver dressing and nonocrystalline silver dressing in rats

Wound healing using ionic silver dressing and nonocrystalline silver dressing in rats

Metronidazole concentration in the bloodstream following its topical application, at different concentration levels, on experimental skin wounds during healing by secondary intention

Effect of metronidazole ophthalmic solution on corneal neovascularization in a rat model

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