Alterações leucocitárias ocasionadas pelo estresse toxicológico agudo do óleo diesel em juvenis de tilápia-do-nilo

Abstract

ABSTRACT:This study aimed to evaluate the stress and changes in white blood cell count of juveniles Oreochromis niloticus subjected to acute toxicity of diesel oil. The toxicity was evaluated in a completely randomized design with five dilutions (20, 25, 30, 35 and 40%) and one control, both with three replicates. For the assessment of blood glucose levels and changes in white blood cell count, blood was drawn by caudal puncture of the dying fish. It was concluded that diesel oil can present risks to aquatic ecosystem, causing toxicity and physiological changes to fish. KEYWORDS: physiological changes; fish; derived oil; leukogram. RESUMO:O presente trabalho teve como objetivo avaliar o estresse e as alterações no leucograma dos juvenis de Oreochromis niloticus submetidos à toxicidade aguda do óleo diesel. A toxicidade foi avaliada em um delineamento inteiramente casualizado com cinco diluições (20, 25, 30, 35 e 40%) e um controle, ambos com três repetições. Para a avaliação dos níveis glicêmicos e alteração no leucograma, foi retirado sangue por punção caudal dos peixes moribundos. Dessa forma, conclui-se que o óleo diesel pode apresentar riscos ao ecossistema aquático, provocando toxicidade aos organismos aquáticos e alterações fisiológicas. Alterações leucocitárias ocasionadas pelo estresse toxicológico agudo do óleo diesel em juvenis de tilápia-do-nilo PALAVRAS-CHAVE:

Chemical exposure hazardous for fish Hyphessobrycon eques through the incorrect release of oil in Amazon region

“…In the present study, changes in glucose values caused by burned oil would be an indicator of stress in fish [18] . This elevation of glucose was probably stimulated by catecholamines, increasing glycogenolysis in order to prepare the animal for rapid action for escape the stressor [18,28] .…”

“…In the present study, changes in glucose values caused by burned oil would be an indicator of stress in fish [18] . This elevation of glucose was probably stimulated by catecholamines, increasing glycogenolysis in order to prepare the animal for rapid action for escape the stressor [18,28] . According to Simonato [11] monitoring the toxicity of diesel FSA dilution of 50% Prochilodus lineatus juveniles for 6, 24 and 96 hours, observed a difference in glucose values with an increase of 172% (24h exposure) and 120% (96 h of exposure) in relation to the control (22±1.00 mg.dL -1 ).…”

“…Different organisms can show different sensibility with toxicity tests until in several development stages [15,17,18] . According to Rodrigues [12] , they observed severe toxicity of brute oil, diesel and gasoline for lavae Odontesthes argentinensis which determined lethal dose 70.68, 13.46 e 5.48%, respectively, showing its toxic effect.…”

“…Thus, both marine and freshwater fish are subject to the toxic effects of FSA from oil products. These compounds dissolved in water are responsible for the risks to the aquatic environment and the organisms affecting homeostatic imbalance, which results in changes in hematological and tissue parameters [11,13,15,18,24 ], may cause stress and later fish mortality.…”

“…For this study, it captured (sisbio license 19515) in the Chumucuí River (01º 08 '40.12" S and 46º 34' 04.8" W) and acclimated in 300 L water tanks species of H. eques for a period of seven days. Afterwards, all fish passed by feeding deprivation before the experiment [15,18] . Preliminary tests determined the FSA dilutions of burned oil that would cause 0 and 100% of mortality.…”