Alterações metabólicas em plantas de feijão originadas de sementes microbiolizadas por Pseudomonas sp. e inoculadas com Xanthomonas axonopodis pv. phaseoli

Abstract

Many enzymes are involved in plant defense a gainst pathogens. T he purpose of this stu dy was to verify altera tions in a ctivity of some enzymes in bea n pla nts origina ted from microbioliza tion of seeds with a fluorescent isolate (DFs84 2) of Pseu do mo na s. Bean seeds (cv. "BRS Valente") were immersed in a bacterial suspension of a 24 hours old Pseudomonas culture (OD 540 =0,5) known as a Xanthomonas axonopodis pv. phaseoli biocontroler. Check treatment consisted of seeds immersed in a saline solution (NaCl 0,85%). After microbiolization for 5 hours at 10 o C, seeds were sowed in a non sterilized substrate composed of soil, sand and bovine manure mixture (3:1:1 ratio) disposed in pots in a greenhouse. The pathogen was inoculated by cutting the third true leaves with scissors previously immersed in the bacterial suspension (X. ax onop odis pv. ph ase oli) prepared from a 24 hou rs old cu ltu re (OD 540 =0,4). Plants were kept in moister chambers for 24 hours before and after the inoculation. For protein extraction preparation, the three Silva E. G.; Moura A. B.; Bacarin, M.A.; Deuner, C.C. Metabolic alterations on bean plants originated from microbiolization of seeds with Pseudomonas sp. and inoculated with Xanthomnas axonopodis pv. phaseoli. Summa Phytopathologica, v.35, n.2, p.98-104, 2009

Induction of defense enzymes and control of anthracnose in cucumber by Corymbia citriodora aqueous extract

“…Increase in FAL is associated with a reduction in the severity of some plant diseases. This was not observed by Silva et al (22), who detected no changes in FAL activity in seed treatment with Bacillus cereus in tomato; however, high peroxidase activity was emphasized. The use of neem (Azadirachta indica) extract promoted an increase in the activity of polifenol oxidase, FAL and peroxidase in carrots, indicating a resistance-inducing effect (17).…”

Foliar spraying with bacterial biocontrol agents for the control of common bacterial blight of bean

“…Several authors have reported the biological control of bacterial diseases caused by Xanthomonas spp. (Naue et al, 2014;Halfeld-Vieira et al, 2015;Singh & Siddiqui, 2015), including the selection of biocontroller bacteria against Xap (Zanatta et al, 2007;Silva et al, 2009).…”

“…The foliar application of bacterial biocontrol agents could improve the control of common bacterial blight of bean. This is the prospect of some bacterial biocontrol agents that control Xap, selected for the treatment of bean seeds (Zanatta et al, 2007), which could increase the levels of compounds related to resistance (Silva et al, 2009), showing broadspectrum effects (Corrêa et al, 2014). However, there is no information available about the behavior of these bacteria when used in foliar spraying.…”