Alterações vasculares em ratos obesos por dieta rica em gordura: papel da Via L-arginina/NO Endotelial

Nascimento, Thiago Bruder do; Baptista, Rafaela de Fátima Ferreira; Pereira, Priscila Cristina; Campos, Dijon Henrique Salomé de; Leopoldo, André Soares; Leopoldo, Ana Paula Lima; Júnior, Silvio A. Oliveira; Padovani, Carlos Roberto; Cicogna, Antônio Carlos; Cordellini, Sandra

doi:10.1590/s0066-782x2011005000063

Cited by 25 publications

(27 citation statements)

References 33 publications

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“…So, augmented vasodilation in obese group could be attributed to an increase of NO release and this is in contrast with previous studies which have reported a decrease of endothelium-dependent relaxation to vasodilators in obesity 32-35. However an increase of eNOS activation 36 and improvement of endothelial-L-arginine/NO pathway have been previously described 16. These effects have been related with hyperinsulinemia and hyperleptinemia associated with obesity since both insulin and leptin increase endothelial NO production 16.…”

Section: Discussionmentioning

confidence: 73%

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Bifidobacterium pseudocatenulatum CECT 7765 supplementation restores altered vascular function in an experimental model of obese mice

et al. 2017

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Aims. Bifidobacterium pseudocatenulatum CECT 7765 improves metabolic and immunological altered functions in high fat fed mice, however little is known about the effects of potential probiotics on vascular reactivity. The aim of the present study was to investigate the effects of a potential probiotic strain, Bifidobacterium pseudocatenulatum CECT 7765, on vascular response in obese mice.Methods. Aorta samples were obtained from mice, which were divided into three groups: a control group, receiving a standard diet; an obese group, receiving a high-fat diet; and an obese group receiving high-fat diet and a daily dose of B. pseudocatenulatum CECT 7765 by oral gavage. Aortic rings were suspended in organ baths for isometric recording of tension. mRNA expression of eNOS was evaluated by real-time polymerase chain reaction.Results. Contractions induced by KCl, noradrenaline and thromboxane analogue were 33%, 30% and 45% lower respectively in aortic rings from obese mice. Bifidobacteria administration reversed this effect. eNOS inhibition increased the response to noradrenaline in the three groups with a significant lower magnitude in aortic rings from obese mice receiving bifidobacteria supplement. Acetylcholine caused a greater vasodilation in aorta from obese group (46±3% for control and 69±4% for obese group; p<0.05) and bifidobacteria reversed it (57±5%). Response to sodium nitroprusside was displaced 2.9 times to the left in a parallel manner in obese group. Relaxation to sodium nitroprusside remained unchanged in the bifidobacteria fed group. There was about five-fold decreased mRNA expression of eNOS in aortic segments from the group receiving bifidobacteria.Conclusion. Bifidobacterium pseudocatenulatum CECT 7765 restores the obesity-induced altered vascular function mainly by reducing nitric oxide release.

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Section: Discussionmentioning

confidence: 73%

“…There are studies demonstrating an increase 15, no change 16 or a decrease in reactivity to agonist vasoconstrictors 17. Differences in pharmacological responses of blood vessels might be receptor-mediated or due to nonspecific responses of smooth muscle.…”

Section: Discussionmentioning

confidence: 99%

Bifidobacterium pseudocatenulatum CECT 7765 supplementation restores altered vascular function in an experimental model of obese mice

et al. 2017

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“…Porém, no presente estudo, todos os grupos iniciaram o protocolo de treinamento e suplementação proteica com valores próximos a 0,6 aumentando para 0,8. Para Angéloco et al 28 , o IMC pode estimar de forma confiável a gordura corporal em rato, sendo diretamente proporcional ao seu crescimento, já o índice de Lee é considerado menos sensível, pois animais com elevada quantidade de massa muscular podem apresentar elevado índice de Lee mesmo que a gordura corporal não seja excessiva [19][20][21] .…”

Section: Discussionunclassified

“…O índice de adiposidade (IA%) foi calculado através da relação entre a gordura corporal total e o peso corporal final multiplicado por 100 20 .…”

Section: Protocolo Experimentalunclassified

“…Nascimento et al 20 induziram a ingestão de dieta rica em gordura durante 30 semanas e compararam com um grupo controle de peso normal, ambos sem exercícios, e verificaram que o índice de adiposidade (%) variou de 4,28 ± 1,65% (grupo controle) a 5,96 ± 1,54% (grupo de ratos obesos). Na presente pesquisa não houve diferença significativa no índice de adiposidade entre os grupos experimentais, podendo-se apenas observar uma tendência de valores maiores para os grupos que receberam whey protein em relação aos grupos que receberam bacuri, mesmo essa dieta contendo maior teor lipídico.…”

Section: Sítios De Gorduraunclassified

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Suplementação Com Amêndoa De Bacuri Na Composição Corporal De Ratos Submetidos Ao Exercício

Rodrigues

Soares

Hiane

et al. 2017

Rev Bras Med Esporte

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RESUMOIntrodução: A proteína da amêndoa do bacuri (Attalea phalerata Mart. Ex Spreng) e da whey protein (WP) têm quantidades relevantes de aminoácidos essenciais e são de boa digestibilidade, características que têm sido buscadas por atletas para auxílio para melhorar a composição corporal, particularmente quando associadas a exercícios. Objetivo: Avaliar o efeito de dietas com teor proteico vegetal e animal sobre a composição corporal de ratos Wistar submetidos ao exercício resistido em escada. Métodos: Quarenta ratos machos recém-desmamados foram submetidos a um período de adaptação com dieta comercial (4 semanas) e treinamento resistido durante oito semanas (três vezes por semana), recebendo dietas com farinha de bacuri e whey protein. Foram distribuídos randomicamente em seis grupos: G1 (bacuri sedentário), G2 (bacuri exercitado), G3 (WP sedentário), G4 (WP exercitado), G5 (controle sedentário) e G6 (controle exercitado). Avaliou-se consumo da dieta, peso corporal, comprimento, circunferências torácica e abdominal para calcular o índice de Lee e o índice de massa corporal; os sítios com gordura foram retirados após eutanásia. A análise estatística foi realizada por ANOVA e Teste de Tukey, considerando 5% de significância. Resultados: O peso final do G3 (372 ± 8,86 g) foi superior ao do G2 (326,83 ± 15,03 g) e do G6 (316,75 ± 5,90 g); os grupos que receberam dieta com farinha de bacuri consumiram quantidade superior de dieta, porém, tiveram menor peso final; não diferiram no índice de Lee e no IMC, sítios com gordura e índice de adiposidade com relação aos grupos tratados com WP, apresentando valores inferiores das circunferências torácica e abdominal. Com relação às gorduras e índice de adiposidade, não houve diferença entre os grupos sedentários e exercitados. Conclusão: A proteína da amêndoa de bacuri mostrou-se de boa qualidade quando comparada com a whey protein, havendo possibilidade de recomendação de seu uso na elaboração de suplementos nutricionais, como fonte vegetal alternativa e de baixo custo.Descritores: proteínas; frutos; composição corporal; exercício. ABSTRACT Introduction: The bacuri almond protein (Attalea phalerata Mart. Ex Spreng) and whey protein (WP) have significant amounts of essential amino acids and are of good digestibility, characteristics that have been sought by athletes to aid in improving body composition, particularly when associated with exercises. Objective: To evaluate the effect of diets with vegetable and animal protein content on the body composition of Wistar rats submitted to resisted ladder exercise. Methods: Forty male newly weaned rats were exposed to a period of adaptation to a commercial diet (4 weeks) and resistance training for eight weeks (three times a week), receiving diets with bacuri flour and whey protein. They were randomly assigned to 6 groups: G1 (sedentary bacuri), G2 (exercised bacuri), G3 (sedentary WP), G4 (exercised WP), G5 (sedentary control) and G6 (exercised control

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The triterpenoid alpha, beta-amyrin prevents the impaired aortic vascular reactivity in high-fat diet-induced obese mice

Santos

Carvalho

Batista‐Lima

et al. 2017

Naunyn-Schmiedeberg's Arch Pharmacol

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To characterize the protective effects of the triterpenoid mixture alpha, beta-amyrin (AMY, 20 mg/kg, during 15 days) on the reactivity of isolated aorta of high-fat diet (HFD)-induced obese mice. Male Swiss mice were fed with HFD or normal diet (ND) for 15 weeks. Contractions of thoracic aorta in response to KCl or phenylephrine (PHE) and relaxation by acetylcholine (ACh) or sodium nitroprusside (SNP) were analyzed. HFD-fed mice developed hyperglycemia, hyperlipidemia, and significant body weight gain, parameters prevented by AMY treatment. Whereas aortic contractility did not differ in response to KCl, contractions induced by PHE (1 μM) as well as relaxation induced by ACh (1-30 μM) or SNP (1 nM-0.1 mM) on PHE-contracted aorta were decreased (p < 0.05) in tissues of HFD compared to ND mice, phenomenon significantly (p < 0.05) diminished in HFD mice treated with AMY. The relaxant actions of ACh and SNP were inhibited (p < 0.05) by tetraethylammonium (TEA, 5 mM), apamin (0.1 μM), and 4-aminopyridine (4-AP; 3 mM) in aortae from ND group, but not from HFD. Treatment of HFD mice with AMY rescued the inhibitory effect of TEA (p < 0.05) on vasorelaxant actions of ACh and SNP. 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) inhibited similarly the relaxant effects of SNP in all groups. 8-Br-cGMP relaxed with similar profile aortae of all groups. By preventing HFD-induced obesity in mice, AMY rescued the blunted contractile response to PHE, and the attenuated vasorelaxation and K channel activation (opening) induced by ACh and SNP in isolated aorta.

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Alterações vasculares em ratos obesos por dieta rica em gordura: papel da Via L-arginina/NO Endotelial

Abstract: Background: Mechanisms underlying vascular abnormalities in obesity remain to be completely clarified.

Cited by 25 publications

References 33 publications

Bifidobacterium pseudocatenulatum CECT 7765 supplementation restores altered vascular function in an experimental model of obese mice

Bifidobacterium pseudocatenulatum CECT 7765 supplementation restores altered vascular function in an experimental model of obese mice

Suplementação Com Amêndoa De Bacuri Na Composição Corporal De Ratos Submetidos Ao Exercício

The triterpenoid alpha, beta-amyrin prevents the impaired aortic vascular reactivity in high-fat diet-induced obese mice

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