Alteration of a recombinant protein N-glycan structure in silkworms by partial suppression of N-acetylglucosaminidase gene expression

Kato, Tatsuya; Kikuta, Kotaro; Kanematsu, Ayumi; Kondo, Sachiko; Yagi, Hirokazu; Kato, Koichi; Park, Enoch Y.

doi:10.1007/s10529-017-2361-y

Cited by 2 publications

(1 citation statement)

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“…In general, the inducible promoter, similar to the transcriptional activator, exists in an inactive form and can be directly or indirectly activated by the corresponding signal. Currently, several technical methods supported by inducible promoters (e.g., Cre-loxp, Tet-On/Tet-Off, and ecdysone and pathogen inducible systems) are widely used in the fields of animal and plant genetic engineering, including gene function identification and a variety of improvements [ 2 – 5 ]. Insects are the largest group of organisms on earth.…”

Section: Introductionmentioning

confidence: 99%

Construction and characterization of a synthetic Baculovirus-inducible 39K promoter

Dong

et al. 2018

J Biol Eng

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BackgroundSilkworm genetic engineering is widely used in gene function, silk engineering and disease-resistant engineering in most of Asia. Some of the earliest promoter elements are used to control the development of silkworm transgenic expression and gene therapy. However, the low expression and specificity of natural promoters limit the applications of genetic engineering. To construct a highly efficient synthetic inducible promoter in the Bombyx mori (Lepidoptera), we analyzed the regulatory elements and functional regions of the B. mori nucleopolyhedrovirus 39 K promoter.ResultsTruncated mutation analysis of the 39 K promoter showed that the transcriptional regulatory region spanning positions − 573 to − 274 and + 1 to + 62 are essential for virus-inducible promoter activity. Further investigations using the electrophoretic mobility shift assay revealed that the baculovirus IE-1 protein binds to the 39 K promoter at the − 310 to − 355 region, and transcription activates the expression of 39 K promoter assay. Finally, we successfully constructed a synthetic inducible promoter that increased the virus-inducing activity of other promoters using the baculovirus-inducible transcriptional activation region that binds to specific core elements of 39 K (i.e., spanning the region − 310 to − 355).ConclusionsIn summary, we constructed a novel, synthetic, and highly efficient biological tool, namely, a virus-inducible 39 K promoter, which provides endless possibilities for future research on gene function, gene therapy, and pest control in genetic engineering.Electronic supplementary materialThe online version of this article (10.1186/s13036-018-0121-8) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Construction and characterization of a synthetic Baculovirus-inducible 39K promoter

Dong

et al. 2018

J Biol Eng

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Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2017–2018

Harvey

2021

Mass Spectrometry Reviews

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This review is the tenth update of the original article published in 1999 on the application of matrix‐assisted laser desorption/ionization mass spectrometry (MALDI) mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2018. Also included are papers that describe methods appropriate to glycan and glycoprotein analysis by MALDI, such as sample preparation techniques, even though the ionization method is not MALDI. Topics covered in the first part of the review include general aspects such as theory of the MALDI process, new methods, matrices, derivatization, MALDI imaging, fragmentation and the use of arrays. The second part of the review is devoted to applications to various structural types such as oligo‐ and poly‐saccharides, glycoproteins, glycolipids, glycosides, and biopharmaceuticals. Most of the applications are presented in tabular form. The third part of the review covers medical and industrial applications of the technique, studies of enzyme reactions, and applications to chemical synthesis. The reported work shows increasing use of combined new techniques such as ion mobility and highlights the impact that MALDI imaging is having across a range of diciplines. MALDI is still an ideal technique for carbohydrate analysis and advancements in the technique and the range of applications continue steady progress.

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Alteration of a recombinant protein N-glycan structure in silkworms by partial suppression of N-acetylglucosaminidase gene expression

Abstract: Suppression of BmFDL gene expression by shRNA is not sufficient to synthesize complex N-glycans in silkworm larvae but can modify the N-glycan synthetic pathway.

Cited by 2 publications

References 42 publications

Construction and characterization of a synthetic Baculovirus-inducible 39K promoter

Construction and characterization of a synthetic Baculovirus-inducible 39K promoter

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2017–2018

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