Alteration of Adipocyte Metabolism in ω3 Fatty Acid-depleted Rats

Oguzhan, Berrin; Sancho, Verónica; Acitores, Alicia; Villanueva-Peñacarrillo, María Luisa; Portois, Laurence; Chardigny, Jean‐Michel; Sener, Abdullah; Carpentier, Yvon; Malaisse, Willy

doi:10.1055/s-2006-956180

Cited by 27 publications

(37 citation statements)

References 10 publications

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“…These rats display, in addition to several features of the metabolic syndrome (15)(16)(17)(18) decreased basal cardiac function and impaired recovery following ischemia (5).…”

Section: Discussionmentioning

confidence: 99%

Alteration of lipid fatty acid profile and cationic fluxes in ventricular cardiomyocytes from ω3-depleted rats

Peltier

Louchami²,

Zhang³

et al. 2009

Int J Mol Med

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Abstract. The bolus intravenous injection of a medium-chain triglyceride:fish oil emulsion was recently found to increase within 60 min the cell phospholipid content in long-chain polyunsaturated ˆ3 fatty acids and, hence, proposed as a potential tool to prevent cardiac arrhythmia in subjects with a decreased dietary intake of such fatty acids. In the present study, ventricular cardiomyocytes from second generation rats depleted in ˆ3 fatty acids were found to display the same changes in the phospholipid fatty acid pattern as that previously documented in the cardiac muscle and endothelium of such rats, altered 86 Rb and 45 Ca fluxes with emphasis on a decrease in both K + inflow and K + content and an increase in both Ca 2+ inflow and content. The alteration of K + inflow could not be attributed to a decrease in ouabain-sensitive Na + ,K + -ATPase activity as measured in cell homogenates. The cationic alterations were corrected, in part at least, by the prior intravenous injection of the medium-chain triglyceride:fish oil emulsion 60 min before sacrifice of the ˆ3-depleted rats.

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“…These rats display, in addition to several features of the metabolic syndrome (15)(16)(17)(18) decreased basal cardiac function and impaired recovery following ischemia (5).…”

Section: Discussionmentioning

confidence: 99%

Alteration of lipid fatty acid profile and cationic fluxes in ventricular cardiomyocytes from ω3-depleted rats

Peltier

Louchami²,

Zhang³

et al. 2009

Int J Mol Med

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“…14 C]glycerol. In the hepatocytes from control animals, no significant correlation was found between the individual values for the total apparent distribution space of [U- 14 C]glycerol and the length of incubation (r= -0.2046; n=78) (Fig. 2).…”

Section: Control Rats N3-pufa-depleted Ratsmentioning

confidence: 94%

“…Glycerol uptake (29) was performed in a 1.5 ml microfuge tube using ~60x10 3 isolated hepatocytes that were incubated at 37˚C for 1-20 min in 0.12 ml Krebs-Ringer bicarbonate buffer containing 0.2 mM [U- 14 C]glycerol 0.7 µCi/ml. At the end of incubation, the hepatocytes were separated by centrifugation at 5000 x g for 3 min following the addition of dibutylphthalate/ di-isononylphthalate (10:3) oils (Sigma-Aldrich, Steinheim, Germany).…”

Section: Real-time Rt-pcrmentioning

confidence: 99%

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Perturbation of glycerol metabolism in hepatocytes from n3-PUFA-depleted rats

et al. 2012

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Abstract. Second generation n3-PUFA-depleted rats represent a good animal model of metabolic syndrome as they display several features of the disease such as liver steatosis, visceral obesity and insulin resistance. The goal of our study was to investigate the influence of n3-PUFA deficiency on hepatic glycerol metabolism. Aquaglyceroporin 9 (AQP9) allows hepatic glycerol transport and consequently contributes to neoglucogenesis. AQP9 knockout mice display hypertriacylglycerolemia, one of the hallmarks of the metabolic syndrome. Our data show reduced AQP9 expression at the protein level in n3-PUFA-depleted rats, without any changes at the mRNA levels. [U-14 C]glycerol uptake was increased in hepatocytes from n3-PUFA-depleted animal cells. The apparent discrepancy between decreased AQP9 protein expression, and increased [U-14 C]glycerol uptake could be explained by an observed increase in glycerol kinase activity.

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“…Rats deprived of a dietary supply of long-chain polyunsaturated ω3 fatty acids also develop a metabolic syndrome with liver steatosis, visceral obesity, insulin resistance, hypertension and resulting cardiac hypertrophy (3)(4)(5)(6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

The metabolic syndrome of fructose-fed rats: Effects of long-chain polyunsaturated ω3 and ω6 fatty acids. I. Intraperitoneal glucose tolerance test

et al. 2011

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Abstract. The present series of experiments aim mainly at investigating the possible influence of changes in the com position of dietary lipids (sunflower oil, salmon oil, safflower oil) upon the metabolic syndrome found in rats exposed to a fructose-rich diet. For purpose of comparison, a control group of rats received the sunflower oil diet with substitution of fructose by starch. An intraperitoneal glucose tolerance test, performed after overnight starvation fifty days after the start of the experiments at the 6th week after birth, indicated, as expected, impaired tolerance to glucose and deterioration of insulin sensitivity (HOMA index), without changes in the insulinogenic index, when comparing the fructose-fed rats to the starch-fed rats both exposed to the sunflower oil diet. In the fructose-fed rats, enrichment of the diet by long-chain polyunsaturated ω3 fatty acids supplied by salmon oil, a modest improvement of insulin sensitivity was opposed, in term of glucose homeostasis, by a decreased secretory response to glucose of insulin-producing cells. Last, in the fructose-fed rats, the partial substitution of sunflower oil by safflower oil rich in long-chain polyunsaturated ω6 fatty acids further deteriorated glucose homeostasis, with a higher mean HOMA index and a severe decrease of the insulinogenic index. These findings justify further investigations on such items as the time course for changes in metabolic and hormonal variables and both the metabolic and secretory responses of isolated pancreatic islets to selected nutrient secretagogues. IntroductionA metabolic syndrome with insulin resistance, hyperinsulinemia and hypertension is known to prevail in rats exposed to a high dietary supply of D-fructose (1). Attention was recently drawn to both the comparison between rats exposed to either a fructose-enriched diet or fructose-enriched drinking water, as well as the reversibility of the fructose-induced metabolic perturbations (2).Rats deprived of a dietary supply of long-chain polyunsaturated ω3 fatty acids also develop a metabolic syndrome with liver steatosis, visceral obesity, insulin resistance, hypertension and resulting cardiac hypertrophy (3-8).The present experiments aim mainly at exploring the effects of long-chain polyunsaturated ω3 and ω6 fatty acids in rats exposed from the 8th week after birth and for the ensuing 8 weeks to a fructose-enriched diet. More precisely, a first comparison deals with rats fed a sunflower oil diet and given access to either a control diet or a fructose-enriched diet obtained by substitution of starch by D-fructose. In this first comparison, the two diets contained 5.0% (w/w) sunflower oil, containing <0.1% of its total fatty acid content as long-chain polyunsaturated ω3 fatty acids. In the second set of com parisons, the just-mentioned rats exposed to the fructose-rich and sunflower oil-containing diet served as the control fructose-fed rats. In another group of rats, part of the sunflower oil (1.6%, w/w) in the fructose-rich diet was replaced by an equal amount...

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Alteration of Adipocyte Metabolism in ω3 Fatty Acid-depleted Rats

Cited by 27 publications

References 10 publications

Alteration of lipid fatty acid profile and cationic fluxes in ventricular cardiomyocytes from ω3-depleted rats

Alteration of lipid fatty acid profile and cationic fluxes in ventricular cardiomyocytes from ω3-depleted rats

Perturbation of glycerol metabolism in hepatocytes from n3-PUFA-depleted rats

The metabolic syndrome of fructose-fed rats: Effects of long-chain polyunsaturated ω3 and ω6 fatty acids. I. Intraperitoneal glucose tolerance test

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