Alteration of ATPase Activity and Duplex DNA in Corneal Cells Grown in High Glucose Media

Whikehart, David R.; Montgomery, Brodrick; Angelos, Phillip; Sorna, Doris H.

doi:10.1097/00003226-199307000-00004

Cited by 18 publications

(14 citation statements)

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“…10 This enzyme is a major component of the endothelial fluid pump. Decreased Na C ,K C -ATPase activity influences the endothelial pump action and induces the dysfunction of the corneal endothelial cell layer.…”

Section: Discussionmentioning

confidence: 99%

“…10 A diabetic endothelium is morphologically abnormal and may be at risk for the change of the corneal endothelial cell morphology during cataract surgical procedures. 11,12 Those with diabetic retinopathy have an increased risk for corneal decompensation after cataract surgery, and the preoperative severity of retinopathy is the best-known predictor of postoperative ocular complications.…”

mentioning

confidence: 99%

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Corneal endothelial cell change after phacoemulsification relative to the severity of diabetic retinopathy

Lee

Choi

et al. 2005

Journal of Cataract and Refractive Surgery

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Corneal endothelial cell change after phacoemulsification relative to the severity of diabetic retinopathy

Lee

Choi

et al. 2005

Journal of Cataract and Refractive Surgery

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“…Repetition of the previous study was considered too expensive and raised ethical concerns in the University Animal Ethics Committee. It should be noted that the long-term decrease in corneal endothelial Na,K ATPase activity found in the earlier diabetic rabbit study (Herse 1990b) has been replicated by other workers using cultured bovine corneal endothelia (Whikehart et al 1993).…”

Section: ) Endothelial Atpase Activitiesmentioning

confidence: 54%

Effect of hyperglycemia duration on rabbit corneal thickness and endothelial ATPase activity

Herse

Adams

1995

Acta Ophthalmologica Scandinavica

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This study compares corneal thickness and corneal endothelial Na,K ATPase activity in normal and age-matched diabetic rabbits with up to 10 weeks of hyperglycemia. Corneal endothelial Na,K ATPase activity in normal rabbits increased between 10 and 20 weeks of age. Corneal thickness increased after 5 weeks of hyperglycemia. Decreased corneal endothelial homogenate Na,K ATPase activity was first seen after 10 weeks of hyperglycemia, though an earlier onset was possible due to sampling restrictions. Corneal endothelial homogenate Mg++ ATPase activity showed a transient increase after 2 to 5 weeks of hyperglycemia. This study shows that hyperglycemia in rabbit is associated with complex dynamic interactions between corneal thickness and corneal endothelial Mg++ ATPase and Na,K ATPase activities.

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“…While the basis of this inhibition is uncertain, it has been suggested that chronic hyperglycemia may result in DNA damage and the formation of dysfunctional or insufficient ATPase (Whikehart et al 1993). It should be noted that short term incubation in hyperglycemic media had no effect on ATPase activities (Whikehart et al 1993).…”

Section: Discussion I) Oxygen Uptakementioning

confidence: 99%

“…Consistent with the methods of Riley et al (1994) and Whikehart et al (1993), the ATPase activities were normalized to homogenate protein levels. It is possible that hyperglycemia may influence protein levels in the corneal epithelium and thus influence measured ATPase activity.…”

Section: Discussion I) Oxygen Uptakementioning

confidence: 99%

Oxygen consumption and ATPase activity in the corneal epithelium of rabbits with alloxan induced hyperglycemia

Herse¹,

Petchell²

1998

Acta Ophthalmologica Scandinavica

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ABSTRACT.Purpose: To more fully investigate the effect of hyperglycemia on the aerobic metabolism of the corneal epithelium. Methods: Corneal epithelial oxygen uptake rates and adenosine triphosphatase (ATPase) activities were measured in alloxan-induced diabetic and control rabbits over a 10 week period. Results: A transient reduction in epithelial oxygen uptake rate was seen at week 1. A chronic 14% reduction in oxygen consumption occurred after 6 weeks of hyperglycemia. Epithelial ouabain-sensitive ATPase activity was unaffected by 10 weeks of hyperglycemia. Epithelial ouabain-insensitive ATPase activity decreased 14% after 10 weeks of hyperglycemia. Conclusions: Ten weeks of hyperglycemia in the alloxan induced diabetic rabbit was associated with a 14% decrease in corneal oxygen uptake, a 14% decrease in corneal epithelial ouabain-insensitive ATPase activity and no change in corneal epithelial ouabain-sensitive ATPase activity. The Crabtree effect may help explain some of the clinical signs seen in the diabetic cornea as well as explaining why diabetic patients can wear contact lenses with minimal clinical problems.

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Alteration of ATPase Activity and Duplex DNA in Corneal Cells Grown in High Glucose Media

Cited by 18 publications

References 0 publications

Corneal endothelial cell change after phacoemulsification relative to the severity of diabetic retinopathy

Corneal endothelial cell change after phacoemulsification relative to the severity of diabetic retinopathy

Effect of hyperglycemia duration on rabbit corneal thickness and endothelial ATPase activity

Oxygen consumption and ATPase activity in the corneal epithelium of rabbits with alloxan induced hyperglycemia

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