Alteration of calcium signalling in cardiomyocyte induced by simulated microgravity and hypergravity

Liu, Caizhi; Zhong, Guangming; Zhou, Yuezhang; Yang, Yuchen; Tan, Yingjun; Gao, Xingcheng; Sun, Weijia; Li, Jianwei; Jin, Xiaoyan; Cao, Dengchao; Yuan, Xinxin; Liu, Zizhong; Liang, Shuai; Li, Youyou; Du, Ronghui; Zhao, Yang; Zhao, Di; Song, Jinping; Ling, Shukuan; Li, Yingxian

doi:10.1111/cpr.12783

Cited by 30 publications

(21 citation statements)

References 66 publications

(131 reference statements)

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“…Furthermore, they exhibited an increased phosphorylation of Ca 2+ /calmodulin-dependent protein kinase II δ (Thr287) and histone deacetylase 4 after 48 h of induced µg. After 48 h of induced µ g , there was also a significant increase in the expression of the foetal genes that encode ANP and brain natriuretic peptide, which indicates remodelling of the myocardia, a decrease in myosin heavy chain α, and thus altered cardiomyocyte structure [ 162 ].…”

Section: Cellular and Molecular Adaptation Of Cardiovascular Cells To Spaceflight (In Vitro)mentioning

confidence: 99%

The Cardiovascular System in Space: Focus on In Vivo and In Vitro Studies

et al. 2021

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On Earth, humans are subjected to a gravitational force that has been an important determinant in human evolution and function. During spaceflight, astronauts are subjected to several hazards including a prolonged state of microgravity that induces a myriad of physiological adaptations leading to orthostatic intolerance. This review summarises all known cardiovascular diseases related to human spaceflight and focusses on the cardiovascular changes related to human spaceflight (in vivo) as well as cellular and molecular changes (in vitro). Upon entering microgravity, cephalad fluid shift occurs and increases the stroke volume (35–46%) and cardiac output (18–41%). Despite this increase, astronauts enter a state of hypovolemia (10–15% decrease in blood volume). The absence of orthostatic pressure and a decrease in arterial pressures reduces the workload of the heart and is believed to be the underlying mechanism for the development of cardiac atrophy in space. Cellular and molecular changes include altered cell shape and endothelial dysfunction through suppressed cellular proliferation as well as increased cell apoptosis and oxidative stress. Human spaceflight is associated with several cardiovascular risk factors. Through the use of microgravity platforms, multiple physiological changes can be studied and stimulate the development of appropriate tools and countermeasures for future human spaceflight missions in low Earth orbit and beyond.

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Section: Cellular and Molecular Adaptation Of Cardiovascular Cells To Spaceflight (In Vitro)mentioning

confidence: 99%

The Cardiovascular System in Space: Focus on In Vivo and In Vitro Studies

et al. 2021

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“…That was equivalent to the MG of low earth orbit (about 0.01g). The 2D clinostat has been described previously 41 .…”

Section: Clinorotation To Simulate Mgmentioning

confidence: 99%

The combined effects of simulated microgravity and X-ray radiation on MC3T3-E1 cells and rat femurs

Dong

Wang

et al. 2021

npj Microgravity

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Microgravity is well-known to induce Osteopenia. However, the combined effects of microgravity and radiation that commonly exist in space have not been broadly elucidated. This research investigates the combined effects on MC3T3-E1 cells and rat femurs. In MC3T3-E1 cells, simulated microgravity and X-ray radiation, alone or combination, show decreased cell activity, increased apoptosis rates by flow cytometric analysis, and decreased Runx2 and increased Caspase-3 mRNA and protein expressions. In rat femurs, simulated microgravity and X-ray radiation, alone or combination, show increased bone loss by micro-CT test and Masson staining, decreased serum BALP levels and Runx2 mRNA expressions, and increased serum CTX-1 levels and Caspase-3 mRNA expressions. The strongest effect is observed in the combined group in MC3T3-E1 cells and rat femurs. These findings suggest that the combination of microgravity and radiation exacerbates the effects of either treatment alone on MC3T3-E1 cells and rat femurs.

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“…Sci. 2020, 10, 8289 2 of 16 of knowledge revealing the tight interplay among mechanical forces, cell metabolism, and cell behavior [1][2][3][4], as well as to put a spotlight on the importance of physical forces on cell physiology. To this concern, microgravity-related cell modifications have been studied generating an increasing body of evidence, which indicates how different aspects of cell physiology are tightly connected with the gravitational force [5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Microgravity-Induced Cell-to-Cell Junctional Contacts Are Counteracted by Antioxidant Compounds in TCam-2 Seminoma Cells

et al. 2020

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The direct impact of microgravity exposure on male germ cells, as well as on their malignant counterparts, has not been largely studied. In previous works, we reported our findings on a cell line derived from a human seminoma lesion (TCam-2 cell line) showing that acute exposure to simulated microgravity altered microtubule orientation, induced autophagy, and modified cell metabolism stimulating ROS production. Moreover, we demonstrated that the antioxidant administration prevented both TCam-2 microgravity-induced microtubule disorientation and autophagy induction. Herein, expanding previous investigations, we report that simulated microgravity exposure for 24 h induced the appearance, at an ultrastructural level, of cell-to-cell junctional contacts that were not detectable in cells grown at 1 g. In line with this result, pan-cadherin immunofluorescence analyzed by confocal microscopy, revealed the clustering of this marker at the plasma membrane level on microgravity exposed TCam-2 cells. The upregulation of cadherin was confirmed by Western blot analyses. Furthermore, we demonstrated that the microgravity-induced ROS increase was responsible for the distribution of cadherin nearby the plasma membrane, together with beta-catenin since the administration of antioxidants prevented this microgravity-dependent phenomenon. These results shed new light on the microgravity-induced modifications of the cell adhesive behavior and highlight the role of ROS as microgravity activated signal molecules.

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Alteration of calcium signalling in cardiomyocyte induced by simulated microgravity and hypergravity

Cited by 30 publications

References 66 publications

The Cardiovascular System in Space: Focus on In Vivo and In Vitro Studies

The Cardiovascular System in Space: Focus on In Vivo and In Vitro Studies

The combined effects of simulated microgravity and X-ray radiation on MC3T3-E1 cells and rat femurs

Microgravity-Induced Cell-to-Cell Junctional Contacts Are Counteracted by Antioxidant Compounds in TCam-2 Seminoma Cells

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