Alteration of enzymes in ageing human fibroblasts in culture. V. Mechanisms of glutathione peroxidase modification

Pigeolet, Etienne; Remacle, José

doi:10.1016/0047-6374(91)90123-h

Cited by 8 publications

(4 citation statements)

References 40 publications

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“…We detected slightly lower GPx mRNA abundance in senescent cells, which might diminish the cellular response to senescence-associated increases in H 2 O 2 . However, it must be noted that dramatic discrepancies between GPx activity, mRNA abundance, and transcription have been reported to exist under some conditions (Mbemba et al, 1985;Pigeolet and Remacle, 1991). Therefore, in at least some types of cells, GPx activity is largely independent of message level, i.e., translational or posttranslational regulation.…”

Section: Discussionmentioning

confidence: 94%

Differences in electron transport potential, antioxidant defenses, and oxidant generation in young and senescent fetal lung fibroblasts (WI-38)

et al. 1999

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The activities and mRNA abundances of enzymes that regulate the rate of electron flow through the electron transport chain (ETC), including NADH dehydrogenase, succinate dehydrogenase, and cytochrome c oxidase, were examined in young and senescent fetal lung fibroblasts (WI-38). We also determined the activities and mRNA abundances of antioxidant defenses including superoxide dismutase, catalase, and glutathione peroxidase. We confirmed our previous report of a senescence-related increase in the abundance of ND4, a mitochondrially encoded subunit of NADH dehydrogenase. The activities of cytochrome c oxidase and NADH dehydrogenase were also elevated in senescent cultures. No differences were observed in the mRNA abundances of COX-1, a mitochondrially encoded subunit of cytochrome c oxidase or of nuclearly encoded subunits of various electron transport components (SD, COX-4, and ND 51). Lucigenin-detected chemiluminescence and H2O2 generation were both elevated in senescent cells. Catalase activity was also elevated in senescent fibroblasts. However, no differences in catalase mRNA abundance were observed. A small decrease in GSH peroxidase (GPx) mRNA abundance was observed in senescent cells. No other changes in the activities or mRNA abundances of any of the antioxidant defenses were observed in early and late passage cultures. The relationships between oxidant generation, mitochondrial enzyme activities, and antioxidant defense observed during proliferative senescence are dissimilar to those detected between fetal and postnatal fibroblasts as well as those found between fibroblast lines obtained from young and old individuals. The relevance of the differences between these models is discussed.

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Section: Discussionmentioning

confidence: 94%

Differences in electron transport potential, antioxidant defenses, and oxidant generation in young and senescent fetal lung fibroblasts (WI-38)

et al. 1999

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“…No alterations were observed in the basal activity of superoxide dismutase (SOD) [6], and experimental studies in rats found no reductions in glutathione peroxidase (GSH-Px) activity during the ageing process [7,81. Furthermore, in a model of aging cells in culture, no decreases in GSH-Px were obtained if sufficient selenomethionine was added to the medium [9]. The fact that there is no correlation between catalase and GSH-Px activities and the maximum life span of various species and that, at least in some ageing models, the activity of these enzymes is not decreased, strongly indicates that ageing is not associated with a shortage of antioxidant enzyme protection in these models.…”

Section: Introductionmentioning

confidence: 84%

Effect of Oxidative Stress on Lymphocytes from Elderly Subjects

Garcı́a-Arumı́¹,

Andreu²,

López-Hellı́n³

et al. 1998

Clinical Science

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1. Oxidative damage has been associated with ageing, but there is no agreement as to whether or not it is produced by a decrease in antioxidant defences with the ageing process. In purified lymphocytes from 47 healthy elderly (75.27 +/- 0.91 years) and 47 healthy young (29.87 +/- 0.53 years) volunteers, we studied the levels of antioxidant enzyme activity (superoxide dismutase, catalase and glutathione peroxidase), protein oxidative damage (as protein carbonyl content) and lysosomal proteolytic activity (cathepsins B, H and L), with and without exposure to oxidative stress produced by 25 mumol/l H2O2. 2. There were no differences in antioxidant enzyme activities in the stressed and non-stressed samples between the young and elderly subjects, indicating that there was no relationship between age and antioxidant enzyme activity even in oxidative stress. However, a dissimilar response to oxidative stress was observed in protein oxidative damage and cathepsin B and L activities, depending on the age of the donor. 3. With these results we conclude that oxidative stress produces greater protein oxidative damage and increased protein degradation in elderly subjects than in young ones; this effect cannot be attributed to dissimilar antioxidant enzyme responses to oxidative stress, since these did not differ between the two age groups.

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“…However, as seen from table 2 data, senescent passage 25, 30 and 35 cells cultured in MEM1 and MEM2 generated about four times more hydrogen peroxide compared to passage 5 cells, and thus senescent cells might require higher concentrations of Se to stimulate their antioxidant enzyme activities. To this end, many studies have reported that sodium selenite supplementation markedly induced GPx activity in cultured fibroblasts [25,45,46,48], cultured rabbit hepatocytes [49], cultured adrenocortical cells [50], and in endothelial cells [51] where both GPx and thioredoxin reductase expression and activities were stimulated.…”

Section: Discussionmentioning

confidence: 99%

“…Many scientists have studied variations in cGPx and CAT activities and hydrogen peroxide generation rates during aging in vivo [22,23,24], and in senescent human fibroblasts cultures near the end of their proliferative lifespan or derived from donors of different ages [25,26,27,28,29,30,31,32]. The results obtained from such studies are highly controversial and prompt further investigation.…”

Section: Introductionmentioning

confidence: 99%

Effect of Selenium Supplementation on Glutathione Peroxidase and Catalase Activities in Senescent Cultured Human Fibroblasts

Ghneim

Al‐Sheikh²

2011

Ann Nutr Metab

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Aims: To investigate the effect of senescence and selenium supplementation on glutathione peroxidase (cGPx) and catalase (CAT) activities, and concurrent hydrogen peroxide (H₂O₂) generation in subcultured human fibroblasts. Methods: cGPx and CAT activities and H₂O₂ levels were assayed in presenescent passage 5 and 10 cells, and in senescent passage 20, 25, 30 and 35 cells cultured in routine medium (MEM1) and supplemented media MEM2 and MEM3 containing normal and triple human plasma levels of Se, respectively. Senescent cells were identified by studying their growth and replication states, and by monitoring their activity of key glucose and glycogen degradative enzymes. Results: cGPx activity showed moderate increases in senescent cells at passages 20–35 subcultured in MEM1 or MEM2. This activity underwent highly significant progressive increases in the same senescent cells subcultured in MEM3. In contrast, CAT activity showed progressive, highly significant increases in senescent cells at passages 20–35 regardless of the culture medium type. Concurrent H₂O₂ generation was significantly increased in passage 15–25 cells and peaked to higher levels in passage 30 and 35 cells cultured in MEM1 or MEM2. These rates, however, were significantly reduced in senescent passage 20–35 cells cultured in MEM3. Conclusions: The highest cGPx activity and coupled lower H₂O₂ generation were achieved in senescent cells cultured in MEM3.

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Alteration of enzymes in ageing human fibroblasts in culture. V. Mechanisms of glutathione peroxidase modification

Cited by 8 publications

References 40 publications

Differences in electron transport potential, antioxidant defenses, and oxidant generation in young and senescent fetal lung fibroblasts (WI-38)

Differences in electron transport potential, antioxidant defenses, and oxidant generation in young and senescent fetal lung fibroblasts (WI-38)

Effect of Oxidative Stress on Lymphocytes from Elderly Subjects

Effect of Selenium Supplementation on Glutathione Peroxidase and Catalase Activities in Senescent Cultured Human Fibroblasts

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