Alteration of hepatic polyribosome structure and function in mice during hypothermia

Gamulin, Stjepan; Narancsik, P.

doi:10.1016/0014-4800(78)90011-4

Cited by 3 publications

(3 citation statements)

References 14 publications

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“…The method used for the analysis of the functional distribution of ribosomes was exactly that described previously [Gamulin and Narancsik, 1978]. One group of 4 mice was injected with [l4C]-and one group of 4 with [3H]-orotic acid.…”

Section: Animalsmentioning

confidence: 99%

“…In experi ments designed to measure the proportion of ribosomes in various functional states, dou ble labelling of RNA was used. The relative incorporation of radioactively labelled leucin into soluble and nascent polypeptides in the livers of cadmium-treated and control mice was measured by using [3H]-and [14C]-leucine [Gamulin and Narancsik, 1978]. …”

Section: Introductionmentioning

confidence: 99%

“…1977: Corel al" 1979, Monomeric ribosome fraction is heteroge nous and consists of unprogrammed (single or run-off) ribosomes and monoribosomes (one ribosome attached to mRNA). Hence a detailed analysis of the distribution of ribo somes between their various functional states (native subunits, unprogrammed ribosomes, monoribosomes and polyribosomes) should include the analysis of the composition of monomeric ribosome fraction [Norman el al" 1973], By analyzing the functional distribution of ribosomes and their protein synthetic ability, it is possible to get some insight into the mechanism interfering with the polyribo some function [Noll, 1969;Vassart et al, 1971;Norman et al, 1973;Gamulin and Narancsik, 1978].…”

Section: Introductionmentioning

confidence: 99%

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Effects of Cadmium on Mouse Liver Polyribosome Function

Gamulin¹,

Narancsik²

1982

Pathobiology

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The distribution of ribosomes between their various functional states (native subunits, unprogrammed ribosomes, monoribosomes and polyribosomes) was analyzed in livers of cadmium-treated (CdCl₂ 20 µmol/kg, 1 h) and control mice. The ribosomes with double-labelled RNA were separated by sucrose density-gradient centrifugation in zonal rotor, and monomeric ribosome fraction was subsequently isolated and analyzed by selective dissociation of unprogrammed ribosomes. The analysis shows that the increase in monomeric ribosomes, occurring during polyribosome disaggregation in livers of cadmium-treated mice, is entirely due to the increase of the unprogrammed ribosome fraction at the expense of polyribosomes. Protein synthetic activity of polyribosomes in vivo in livers of cadmium-treated and control mice were compared by double labelling of nascent and soluble polypeptides with [¹⁴C]-and [³H]-leucine. Incorporation of radioactivity was relatively higher in nascent polypeptides and lower in soluble polypeptides in cadmium-treated as compared with control mice. The results indicate that cadmium inhibits protein synthesis in livers of mice, affecting both the rate of initiation and the rate of elongation, but decreasing the former more than the latter.

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Section: Animalsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of Cadmium on Mouse Liver Polyribosome Function

Gamulin¹,

Narancsik²

1982

Pathobiology

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Die Regulation der Proteinsynthese am normalen Herzen und unter pathologischen Bedingungen

Zähringer¹

1984

Herzinsuffizienz

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Influence of starvation and total protein deprivation on cardiac mRNA levels

et al. 1985

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The effect of starvation and of protein-deprivation on the extractable amount of cardiac mRNA was investigated in male rats. Cardiac mRNA was determined by either (a) isolation of cardiac mRNA by SDS-Phenol/oligo-dT-cellulose, or by (b) hybridization of cardiac mRNA to 3H-Poly(U). During starvation (1-6 days) the extractable amount of cardiac microsomal RNA decreased from 870 micrograms/g heart (controls) to 606 micrograms/g (3 days) and to 547 micrograms/g (6 days), the extractable amount of mRNA fell from 28.6 micrograms/g heart (controls) to 18.7 micrograms/g (3 days) and to 14.5 micrograms/g (6 days). When a normocaloric but protein-deficient diet was fed, the decreases in cardiac microsomal RNA and mRNA were qualitatively similar, but slightly less severe. An analysis of the intracellular distribution of cardiac microsomal RNA and mRNA in the hearts of normal animals and of animals starved or fed a protein-deficient diet indicates that during starvation cardiac mRNA does not accumulate in the cell sap, but gets rapidly degraded. In the refeeding period, mRNA is transported from the nucleus to the cytoplasm and engages in polyribosome formation. The specific mRNA species coding for the major myofibrillar cardiac proteins are affected to a similar extent by these changes during starvation/protein-deprivation and refeeding.

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Alteration of hepatic polyribosome structure and function in mice during hypothermia

Cited by 3 publications

References 14 publications

Effects of Cadmium on Mouse Liver Polyribosome Function

Effects of Cadmium on Mouse Liver Polyribosome Function

Die Regulation der Proteinsynthese am normalen Herzen und unter pathologischen Bedingungen

Influence of starvation and total protein deprivation on cardiac mRNA levels

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