Alteration of HSF3 and HSP70 mRNA expression in the tissues of two chicken breeds during acute heat stress

Abstract

ABSTRACT. This study aimed to estimate changes in HSF3 and HSP70 mRNA expression in stress-sensitive tissues of 2 chicken breeds during acute heat stress. Lingshan chickens (LSC) and White Recessive Rock (WRR) (24 chickens of each breed) were randomly divided into 4 groups (0, 2, 3, and 6 h of heat treatment). With increasing heat treatment time, both HSF3 and HSP70 expression first declined and then showed a significant increase in both breeds. However, HSP70 expression decreased in the heart following 6 h of heat treatment, whereas HSF3 expression continued to increase. After 2 h of heat treatment, HSF3 expression was significantly higher in the brain and leg muscle of LSC compared to WRR (P < 0.05, P < 0.01). In comparison, HSP70 expression was significantly higher in the liver and leg muscle of WRR compared to LSC (P < 0.01, P < 0.05). After 3 h of heat treatment, HSF3 expression was significantly higher in the brain and leg muscle of LSC compared to WRR (P < 0.01). In comparison, HSP70 expression was significantly higher in the liver and heart of LSC compared to WRR (P < 0.01). These results indicate that the expression of HSF3 and HSP70 mRNA in LSC and WRR exhibit species-specific and tissue-specific differences during heat treatment.

Differences in Susceptibility to Heat Stress along the Chicken Intestine and the Protective Effects of Galacto-Oligosaccharides

“…In contrast to the results in jejunum and ileum, no heat stress-related alterations in HSP70 and HSP90 mRNA expression were observed in duodenum and colon, indicating again differences in the susceptibility of the individual parts of the intestines. These findings are in line with a study Zhang et al, [ 43 ] in which differences in the mRNA expression of HSF3 and HSP70 between two chicken breeds and tissue-specific differences during heat treatment are described.…”

Upregulation of TLR4 mRNA Expression Levels in Broiler Chickens Under Acute Heat Stress

“…Many studies have demonstrated the damage or injury of the heart, liver, and brain tissues, as well as and angiocarpy in chickens and rats under HS (Zhang et al, 2014;Quinn et al, 2015;Ito et al, 2015). Moreover, AHS triggers the expression of heat stress-related genes, such as HSP70, HSP70s, TLR2 and TLR4 (Ju et al, 2014;Zhang et al, 2014;Richter et al, 2015;Zhang et al, 2015).…”

“…It well known that poultry species, particularly broiler chickens, are more sensitive to high environmental temperatures and humidity levels than other domestic animals because of their feather cover, lack of sudoriferous glands, and fast growth rate (Piestun et al, 2013;Di et al, 2015). When the environmental temperature exceeds the optimal temperature range, broilers show signs of heat stress, including rise in body temperature, reduced feed intake, poor reproductive and growth performance, immunosuppression, and increased mortality (Kamboh et al, 2013;Zhao et al, 2013;Zhang et al, 2014;Lasagna et al, 2015;Li et al, 2015;Sun et al, 2015). Many studies have demonstrated the damage or injury of the heart, liver, and brain tissues, as well as and angiocarpy in chickens and rats under HS (Zhang et al, 2014;Quinn et al, 2015;Ito et al, 2015).…”

“…However, many animal species typically have a protective metabolism that is adapted to the optimal temperature range of the environment in which they have evolved (Zhang et al, 2014;Sun et al, 2015). It well known that poultry species, particularly broiler chickens, are more sensitive to high environmental temperatures and humidity levels than other domestic animals because of their feather cover, lack of sudoriferous glands, and fast growth rate (Piestun et al, 2013;Di et al, 2015).…”

“…When the environmental temperature exceeds the optimal temperature range, broilers show signs of heat stress, including rise in body temperature, reduced feed intake, poor reproductive and growth performance, immunosuppression, and increased mortality (Kamboh et al, 2013;Zhao et al, 2013;Zhang et al, 2014;Lasagna et al, 2015;Li et al, 2015;Sun et al, 2015). Many studies have demonstrated the damage or injury of the heart, liver, and brain tissues, as well as and angiocarpy in chickens and rats under HS (Zhang et al, 2014;Quinn et al, 2015;Ito et al, 2015). Moreover, AHS triggers the expression of heat stress-related genes, such as HSP70, HSP70s, TLR2 and TLR4 (Ju et al, 2014;Zhang et al, 2014;Richter et al, 2015;Zhang et al, 2015).…”