Alteration of nanomechanical properties of pancreatic cancer cells through anticancer drug treatment revealed by atomic force microscopy

Liang, Xiaoteng; Liu, Shuai; Wang, Xiuchao; Xia, Dan; Li, Qiang

doi:10.3762/bjnano.12.101

Cited by 5 publications

(5 citation statements)

References 31 publications

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“…Notably, the expression levels of these genes appear to correlate with cell elasticity. For example, the elasticity of pancreatic cancer cells was in the order of BxPC-3 > MIA-PaCa-2 > AsPC-1 ( 58 ). This order aligns with the expression patterns of genes, such as CNN2 (Calponin 2, binds to actin filaments and is involved in the regulation of smooth muscle contraction), TPM2 (Tropomyosin 2, an actin filament binding protein associated with the cytoskeleton and is important in controlling cell shape and mechanical properties of muscle cells) and LMNA (Lamin A/C, provides mechanical support to the nucleus and plays a role in gene regulation) ( Figure 4B ).…”

Section: Resultsmentioning

confidence: 99%

MechanoBase: a comprehensive database for the mechanics of tissues and cells

Xiong,

Li,

Zhang

et al. 2024

Database

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Mechanical aspects of tissues and cells critically influence a myriad of biological processes and can substantially alter the course of diverse diseases. The emergence of diverse methodologies adapted from physical science now permits the precise quantification of the cellular forces and the mechanical properties of tissues and cells. Despite the rising interest in tissue and cellular mechanics across fields like biology, bioengineering and medicine, there remains a noticeable absence of a comprehensive and readily accessible repository of this pertinent information. To fill this gap, we present MechanoBase, a comprehensive tissue and cellular mechanics database, curating 57 480 records from 5634 PubMed articles. The records archived in MechanoBase encompass a range of mechanical properties and forces, such as modulus and tractions, which have been measured utilizing various technical approaches. These measurements span hundreds of biosamples across more than 400 species studied under diverse conditions. Aiming for broad applicability, we design MechanoBase with user-friendly search, browsing and data download features, making it a versatile tool for exploring biomechanical attributes in various biological contexts. Moreover, we add complementary resources, including the principles of popular techniques, the concepts of mechanobiology terms and the cellular and tissue-level expression of related genes, offering scientists unprecedented access to a wealth of knowledge in this field of research. Database URL: https://zhanglab-web.tongji.edu.cn/mechanobase/ and https://compbio-zhanglab.org/mechanobase/

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Section: Resultsmentioning

confidence: 99%

MechanoBase: a comprehensive database for the mechanics of tissues and cells

Xiong,

Li,

Zhang

et al. 2024

Database

View full text Add to dashboard Cite

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“…Similarly, treatment of pancreatic cancer cells with progressively higher concentrations of the anticancer drug doxorubicin hydrochloride (DOX) resulted in a subsequent increase in Young's modulus of the cells, which was more inclined to the stiffness of normal pancreatic cells (Liang et al, 2021).…”

Section: Khan Et Al Used Superparamagnetic Iron Oxide Nanoparticles (...mentioning

confidence: 99%

“…By AFM measurements, they found that the use of SP‐CUR significantly increased the stiffness of cancer cells, which suggests a decrease in cancer cell invasiveness after drug administration (Khan et al, 2019). Similarly, treatment of pancreatic cancer cells with progressively higher concentrations of the anticancer drug doxorubicin hydrochloride (DOX) resulted in a subsequent increase in Young's modulus of the cells, which was more inclined to the stiffness of normal pancreatic cells (Liang et al, 2021).…”

Section: Cancermentioning

confidence: 99%

Atomic force microscopy in disease‐related studies: Exploring tissue and cell mechanics

Liu,

Han,

Kong

et al. 2023

Microscopy Res & Technique

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Despite significant progress in human medicine, certain diseases remain challenging to promptly diagnose and treat. Hence, the imperative lies in the development of more exhaustive criteria and tools. Tissue and cellular mechanics exhibit distinctive traits in both normal and pathological states, suggesting that “force” represents a promising and distinctive target for disease diagnosis and treatment. Atomic force microscopy (AFM) holds great promise as a prospective clinical medical device due to its capability to concurrently assess surface morphology and mechanical characteristics of biological specimens within a physiological setting. This review presents a comprehensive examination of the operational principles of AFM and diverse mechanical models, focusing on its applications in investigating tissue and cellular mechanics associated with prevalent diseases. The findings from these studies lay a solid groundwork for potential clinical implementations of AFM.Research HighlightsBy examining the surface morphology and assessing tissue and cellular mechanics of biological specimens in a physiological setting, AFM shows promise as a clinical device to diagnose and treat challenging diseases.

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“…However, the cellular mechanistic properties that allow cells to express various biological functions have not been well appreciated [16]. In recent years, alterations in the physical properties of cells have been considered as a marker of malignant transformation of cancer cells [17][18][19]. Based on atomic force microscopy (AFM) measurements, our group found that the progression of prostate cancer tissue is related to its biomechanical properties (i.e., the higher the pathological grade of prostate cancer tissue, the less elastic and viscous it is) indicating that the mechanical properties of the tissue can predict the pathological grade of prostate cancer.…”

Section: Introductionmentioning

confidence: 99%

Effects of substrate stiffness on the viscoelasticity and migration of prostate cancer cells examined by atomic force microscopy

Tang¹,

Zhang²,

Mao³

et al. 2022

Beilstein J. Nanotechnol.

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The stiffness of the extracellular matrix of tumour cells plays a key role in tumour cell metastasis. However, it is unclear how mechanical properties regulate the cellular response to the environmental matrix. In this study, atomic force microscopy (AFM) and laser confocal imaging were used to qualitatively evaluate the relationship between substrate stiffness and migration of prostate cancer (PCa) cells. Cells cultured on stiff substrates (35 kPa) undergone several interesting phenomena compared to those on soft substrates (3 kPa). Here, the stimulation generated by the stiff substrates triggered the F-actin skeleton to bundle its filaments, increasing the polarity index of the external contour of PCa cells. Analysis of AFM force–distance curves indicated that the elasticity of the cells cultured on 35 kPa substrates increased while the viscosity decreased. Wound-healing experiments showed that PCa cells cultured on 35 kPa substrates have higher migration potential. These phenomena suggested that the mechanical properties may be correlated with the migration of PCa cells. After actin depolymerisation, the elasticity of the PCa cells decreased while the viscosity increased, and the migration ability was correspondingly decreased. In conclusion, this study clearly demonstrated the relationship between substrate stiffness and the mechanical properties of cells in prostate tumour metastasis, providing a basis for understanding the changes in the biomechanical properties at a single-cell level.

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Alteration of nanomechanical properties of pancreatic cancer cells through anticancer drug treatment revealed by atomic force microscopy

Cited by 5 publications

References 31 publications

MechanoBase: a comprehensive database for the mechanics of tissues and cells

MechanoBase: a comprehensive database for the mechanics of tissues and cells

Atomic force microscopy in disease‐related studies: Exploring tissue and cell mechanics

Effects of substrate stiffness on the viscoelasticity and migration of prostate cancer cells examined by atomic force microscopy

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