Alteration of rat adipose tissue lipolytic response to norepinephrine by dietary fatty acid manipulation

Awad, Atif B.; Zepp, E.Andrew

doi:10.1016/0006-291x(79)90392-9

Cited by 28 publications

(3 citation statements)

References 12 publications

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“…Matsuo and Suzuki68 demonstrated altered affinities of β-adrenergic receptors in brown adipose tissue, the heart, and soleus muscle as a consequence of a high-fat diet rich in saturated fatty acids. Awad and Zepp69 demonstrated that rats fed with a saturated fatty acid-rich diet featured lower lipolysis rates than animals fed with a polyunsaturated fatty acid-rich diet, due to a lower activity of hormone sensitive lipase. Others have also indicated that saturated fatty acid intake enhances body fat storage accumulation by a reduction of sympathetic activity in brown adipose tissue, the heart, and skeletal muscle 68,70…”

Section: Obesity-inducing Factorsmentioning

confidence: 99%

Obesity: considerations about etiology, metabolism, and the use of experimental models

Pereira-Lancha¹,

Campos-Ferraz²,

Lancha³

2012

DMSO

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Studies have been conducted in order to identify the main factors that contribute to the development of obesity. The role of genetics has also been extensively studied. However, the substantial augmentation of obesity prevalence in the last 20 years cannot be justified only by genetic alterations that, theoretically, would have occurred in such a short time. Thus, the difference in obesity prevalence in various population groups is also related to environmental factors, especially diet and the reduction of physical activity. These aspects, interacting or not with genetic factors, could explain the excess of body fat in large proportions worldwide. This article will focus on positive energy balance, high-fat diet, alteration in appetite control hormones, insulin resistance, amino acids metabolism, and the limitation of the experimental models to address this complex issue.

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Section: Obesity-inducing Factorsmentioning

confidence: 99%

Obesity: considerations about etiology, metabolism, and the use of experimental models

Pereira-Lancha¹,

Campos-Ferraz²,

Lancha³

2012

DMSO

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“…Other studies suggest that it is the nature of the fatty acids rather than the amount of fat in the diet which is important, as diets rich in polyunsaturated fats result in a much greater lipolytic response to catecholamines than diets rich in saturated fat (Carreau et al 1972;Awad & Zepp, 1979;Awad & Chattopadhyay, 1986;Parrish et al 1991). Decreased adenylate cyclase, cyclic-AMP phosphodiesterase and hormone-sensitive lipase (Awad & Chattopadhyay, 1986) may all contribute to the decreased responsiveness of fat cells from rats fed on diets rich in saturated fats.…”

Section: Effects Of Dietmentioning

confidence: 99%

Effects of diet on lipolysis and its regulation

Vernon

1992

Proc. Nutr. Soc.

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The amount of lipid in a fat cell (adipocyte) is determined by the rate of lipolysis as well as the rate of lipid synthesis. Both processes occur simultaneously and continuously, their relative rates determining if there is net lipid loss or accretion. REGULATIONS OF LIPOLYSISAcute control. Hydrolysis of triacylglycerols is catalysed by hormone-sensitive lipase. The activity of this enzyme is enhanced by phosphorylation by cyclic-AMP-dependent kinase (A-kinase). Hormone-sensitive lipase, and hence lipolysis, is thus acutely stimulated by a variety of hormones (e.g. adrenaline, glucagon, adrenocorticotrophic hormone (ACTH)) and the neurohumoral transmitter, noradrenaline, which increase the adenylate cyclase-A-kinase signal transduction system. Each peptide hormone interacts with its own receptor in the plasma membrane while adrenaline and noradrenaline interact with the P-adrenergic receptor. These hormone-receptor interactions lead to dissociation and activation of the GTP-binding protein, G,, which in turn leads to activation of adenylate cyclase (also located in the plasma membrane) and the synthesis of cyclic-AMP (Fig. 1). Cyclic-AMP activates A-kinase which in turn phosphorylates and activates hormone-sensitive lipase. Signal transduction through this system is modulated by noradrenaline and adrenaline acting via a second receptor, the 1x2-adrenergic receptor, and by prostaglandins (El and E2) and adenosine, both produced within adipose tissue, and acting via their own receptors. These receptors are coupled to a second GTP-binding protein, Gi. Receptor activation leads to dissociation of Gi which inhibits adenylate cyclase (Fig. 1). Catecholamines can, thus, both stimulate and inhibit lipolysis, the net effect depending on the relative numbers of p-and cx2-adrenergic

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“…The lipid compositions of liver plasma membranes from animals fed either a high-glucose or a high-lard diet are also different (Sun et al, 1979). Recently, the response to noradrenaline in fat-pads was shown to be abolished by feeding a diet high in saturated fatty acids (Awad & Zepp, 1979).…”

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confidence: 99%

Dietary effects on the formation of dolichyl monophosphate mannose by microsomal preparations of rat adipose tissue

Lucas

Tepperman

1980

Biochemical Journal

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Microsomal preparations from rat adipose tissue catalyse the transfer of [(14)C]mannose from GDP-[(14)C]mannose to an endogenous acceptor forming a [(14)C]mannosyl lipid. The mannosyl lipid co-chromatographs with hen oviduct dolichyl monophosphate beta-mannose on three solvent systems. It is stable to mild alkaline hydrolysis, but strong alkaline treatment yields a compound that co-migrates with mannose 1-phosphate. The mannosyl lipid is labile to mild acid hydrolysis, yielding [(14)C]mannose. Formation of the compound is reversible by GDP, but not GMP, and is stimulated by exogenous dolichyl phosphate. The kinetics of transfer of [(14)C]mannose from GDP-[(14)C]mannose to form dolichyl monophosphate mannose were studied by using preparations derived from rats fed on one of four diets: G (high glucose), L (high lard), F (fructose) or GC (high glucose, 0.9% cholesterol). The K(m) and V(max.) values for transfer from GDP-mannose were virtually indistinguishable in the four preparations.In the absence of exogenous dolichyl phosphate, the largest amount of transfer of [(14)C]mannose into the mannosyl lipid was observed with preparations from fructose-fed animals. Preparations from glucose-fed animals showed about 60% as much transfer, whereas membranes from rats fed the other diets showed intermediate values between the fructose- and glucose-fed animals. The inclusion of cholesterol in the glucose diet elicited an increase in transfer of mannose. Under conditions of saturating exogenous dolichyl phosphate, preparations from lard-fed animals have 1.5 times as much enzyme activity as do preparations from animals fed the other three diets.

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Alteration of rat adipose tissue lipolytic response to norepinephrine by dietary fatty acid manipulation

Cited by 28 publications

References 12 publications

Obesity: considerations about etiology, metabolism, and the use of experimental models

Obesity: considerations about etiology, metabolism, and the use of experimental models

Effects of diet on lipolysis and its regulation

Dietary effects on the formation of dolichyl monophosphate mannose by microsomal preparations of rat adipose tissue

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