Alteration of rat hippocampal neurogenesis and neuronal nitric oxide synthase expression upon prenatal exposure to tamoxifen

Nobakht, Maliheh; Gharavi, Mohammad Javad; Mousavizadeh, Kazem; Bakhshayesh, Masoomeh; Ghafourifar, Pedram

doi:10.1016/j.pathophys.2011.01.002

Cited by 4 publications

(3 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies have yielded mixed results regarding effects of TAM on neurons. When injected into pregnant rats, TAM inhibits neurogenesis in the CA1 region of the embryonic hippocampus, 17,18 a result that is compatible with our observations. Also, in isolated rat hippocampal brain slices, incubation with 1 mM TAM for 4 h reduces ATP levels.…”

Section: Discussionsupporting

confidence: 90%

“…16 Consistent with this idea, pre-or neonatal exposure to TAM inhibits neurogenesis in the hippocampus. 17,18 In contrast, neuroprotective effects of TAM have been observed in certain model systems including cerebral ischemia, 19,20 spinal cord injury, 21 and brain injury, 22 and TAM protects cultured rat cortical neurons against toxicity induced by manganese. 23 Thus, TAM can have harmful or protective effects on neurons, depending on the context, and the mechanisms by which TAM induces PCCI remain to be elucidated.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Assay of Calcium Transients and Synapses in Rat Hippocampal Neurons by Kinetic Image Cytometry and High-Content Analysis: An In Vitro Model System for Postchemotherapy Cognitive Impairment

McDonough

Prigozhina

Basa

et al. 2017

ASSAY and Drug Development Technologies

View full text Add to dashboard Cite

Postchemotherapy cognitive impairment (PCCI) is commonly exhibited by cancer patients treated with a variety of chemotherapeutic agents, including the endocrine disruptor tamoxifen (TAM). The etiology of PCCI is poorly understood. Our goal was to develop high-throughput assay methods to test the effects of chemicals on neuronal function applicable to PCCI. Rat hippocampal neurons (RHNs) were plated in 96- or 384-well dishes and exposed to test compounds (forskolin [FSK], 17β-estradiol [ES]), TAM or fulvestrant [FUL], aka ICI 182,780) for 6-14 days. Kinetic Image Cytometry™ (KIC™) methods were developed to quantify spontaneously occurring intracellular calcium transients representing the activity of the neurons, and high-content analysis (HCA) methods were developed to quantify the expression, colocalization, and puncta formed by synaptic proteins (postsynaptic density protein-95 [PSD-95] and presynaptic protein Synapsin-1 [Syn-1]). As quantified by KIC, FSK increased the occurrence and synchronization of the calcium transients indicating stimulatory effects on RHN activity, whereas TAM had inhibitory effects. As quantified by HCA, FSK also increased PSD-95 puncta and PSD-95:Syn-1 colocalization, whereas ES increased the puncta of both PSD-95 and Syn-1 with little effect on colocalization. The estrogen receptor antagonist FUL also increased PSD-95 puncta. In contrast, TAM reduced Syn-1 and PSD-95:Syn-1 colocalization, consistent with its inhibitory effects on the calcium transients. Thus TAM reduced activity and synapse formation by the RHNs, which may relate to the ability of this agent to cause PCCI. The results illustrate that KIC and HCA can be used to quantify neurotoxic and neuroprotective effects of chemicals in RHNs to investigate mechanisms and potential therapeutics for PCCI.

show abstract

Section: Discussionsupporting

confidence: 90%

mentioning

confidence: 99%

Assay of Calcium Transients and Synapses in Rat Hippocampal Neurons by Kinetic Image Cytometry and High-Content Analysis: An In Vitro Model System for Postchemotherapy Cognitive Impairment

McDonough

Prigozhina

Basa

et al. 2017

ASSAY and Drug Development Technologies

View full text Add to dashboard Cite

show abstract

“…[169][170][171] To determine whether exposure of a young brain to a volatile anesthetic impacts neuronal nitric oxide synthase expression, Feng et al 172 exposed young (postnatal day 7) rats to either air or sevoflurane 2.3% in air for 6 hours. [169][170][171] To determine whether exposure of a young brain to a volatile anesthetic impacts neuronal nitric oxide synthase expression, Feng et al 172 exposed young (postnatal day 7) rats to either air or sevoflurane 2.3% in air for 6 hours.…”

Section: Neurotoxicity and Neuroprotection Anesthetic Neurotoxicity Imentioning

confidence: 99%

Neuroanesthesiology Update

Pasternak¹,

Lanier²

2013

Journal of Neurosurgical Anesthesiology

View full text Add to dashboard Cite

We provide a review of both clinical and basic science literature from 2012 relevant to care of the patient with neurological disease. Our review addresses the following major areas: general neurosurgical procedures, stroke, traumatic brain injury, spine surgery, anesthetic neurotoxicity, neuroprotective strategies, electrophysiological monitoring, history, and graduate medical education. We have focused on research describing new and innovative concepts and recurring themes. This review is intended to be of interest to those working in the clinical arena and also to neuroscientists.

show abstract

Nitric oxide pathway as a plausible therapeutic target in autism spectrum disorders

Mehta

Kuhad

Bhandari

2022

Expert Opinion on Therapeutic Targets

View full text Add to dashboard Cite

Alteration of rat hippocampal neurogenesis and neuronal nitric oxide synthase expression upon prenatal exposure to tamoxifen

Cited by 4 publications

References 44 publications

Assay of Calcium Transients and Synapses in Rat Hippocampal Neurons by Kinetic Image Cytometry and High-Content Analysis: An In Vitro Model System for Postchemotherapy Cognitive Impairment

Assay of Calcium Transients and Synapses in Rat Hippocampal Neurons by Kinetic Image Cytometry and High-Content Analysis: An In Vitro Model System for Postchemotherapy Cognitive Impairment

Neuroanesthesiology Update

Nitric oxide pathway as a plausible therapeutic target in autism spectrum disorders

Contact Info

Product

Resources

About