Alteration of red cell membrane organization in sickle cell anaemia

Choe, Hyeryun; Schlegel, Robert; Rubin, Elizabeth; Williamson, Patrick; Westerman, Maxwell P.

doi:10.1111/j.1365-2141.1986.tb07560.x

Cited by 50 publications

(17 citation statements)

References 29 publications

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“…For instance, certain disease states result in the appearance of PS in the outer leaflet of erythrocytes, which causes them to adhere to the endothelium39–41. In addition, PSR, the receptor that recognizes PS in the outer leaflet of apoptotic bodies and allows their removal42, 43, has been identified in fibroblasts, epithelial, and HeLa cells42, all of which are used regularly for adherent cell culture.…”

Section: Resultsmentioning

confidence: 99%

Cell Attachment Behavior on Solid and Fluid Substrates Exhibiting Spatial Patterns of Physical Properties

Oliver¹,

Ngassam²,

Dang³

et al. 2009

Langmuir

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The ability to direct proliferation and growth of living cells using chemically and topologically textured surfaces is finding many niche applications, both in fundamental biophysical investigations of cell-surface attachment as well as in developing design principles for many tissue engineering applications. Here we address cellular adhesion behavior on solid patterns of differing wettability (a static substrate) and fluid patterns of membrane topology (a dynamic substrate). We find striking differences in the cellular adhesion characteristics of lipid mono- and bilayers, despite their essentially identical surface chemical and structural character. These differences point to the importance of subtle variations in the physical properties of the lipid mono- and bilayers (e.g., membrane tension and out-of-plane undulations). Furthermore, we find that introducing phosphatidylserine into the patterned lipidic substrates causes a loss of cell-patterning capability. Implications of this finding for the mechanism by which phosphatidylserine promotes cellular adhesion are discussed.

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Section: Resultsmentioning

confidence: 99%

Cell Attachment Behavior on Solid and Fluid Substrates Exhibiting Spatial Patterns of Physical Properties

Oliver¹,

Ngassam²,

Dang³

et al. 2009

Langmuir

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“…Abnormal PS exposure has been associated with sickle cell disease for many years. One source of this exposure is the release of small vesicles from sickle erythrocytes; 149,150 these vesicles become lipid symmetric, 151 perhaps because of ATP depletion arising from their high surface/volume ratios or perhaps during the fusion process by which they are released. In addition, it has become clear that PS exposure also occurs in a small fraction of circulating sickle cells 152,153 which may contribute to the anemia as well as the hypercoaguability 154 associated with the disease.…”

Section: Cell Death and Differentiationmentioning

confidence: 99%

Phosphatidylserine, a death knell

2001

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Virtually every cell in the body restricts phosphatidylserine (PS) to the inner leaflet of the plasma membrane by energydependent transport from the outer to the inner leaflet of the bilayer. Apoptotic cells of all types rapidly randomize the asymmetric distribution, bringing PS to the surface where it serves as a signal for phagocytosis. A myriad of phagocyte receptors have been implicated in the recognition of apoptotic cells, among them a PS receptor, yet few ligands other than PS have been identified on the apoptotic cell surface. Since apoptosis and the associated exposure of PS on the cell surface is probably over 600 million years old, it is not surprising that evolution has appropriated aspects of this process for specialized purposes such as blood coagulation, membrane fusion and erythrocyte differentiation. Failure to efficiently remove apoptotic cells may contribute to inflammatory responses and autoimmune diseases resulting from chronic, inappropriate exposure of PS. Cell Death and Differentiation (2001) 8, 551 ± 563.

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“…In contrast, there are limited data examining alterations in the lateral distribution of phospholipids in the plane of the membrane. Labeling of membrane lipids by merocyanine, a probe of lipid packing (20)(21)(22), has demonstrated that the merocyanine fluorescence is preferentially localized in vesicles which are released from the spicules of deoxygenated sickled red cells upon reoxygenation suggesting that membrane lipid asymmetry is disrupted in the spicules (23).…”

Section: Introductionmentioning

confidence: 99%