Alteration of Seed Fatty Acid Composition by an Ethyl Methanesulfonate-Induced Mutation in Arabidopsis thaliana Affecting Diacylglycerol Acyltransferase Activity

Katavić, Vesna; Reed, Darwin W.; Taylor, David C.; Giblin, E. Michael; Barton, Dennis L.; Zou, Jitao; MacKenzie, S. L.; Covello, Patrick S.; Kunst, Ljerka

doi:10.1104/pp.108.1.399

Cited by 254 publications

(280 citation statements)

References 33 publications

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“…This is consistent with gene expression data, which show that SDP1 transcripts are greater than 10-fold more abundant in vegetative tissues than SDP1L (Kelly et al, 2011). TAG synthesis in Arabidopsis seeds relies jointly on the activities of DGAT1 (Katavic et al, 1995) and PDAT1 ). However, analysis of dgat1 and pdat1 mutants suggests that DGAT1 is quantitatively more important ).…”

Section: Tag Accumulation In Sdp1 Roots Is Greater Than In Several Otsupporting

confidence: 90%

“…Finally, analysis of sdp1 dgat1 and sdp1 pdat1 double mutants showed that TAG accumulation in sdp1 roots is largely dependent on DGAT1 function but that PDAT1 is also required. It has already been established that DGAT1 is necessary for normal TAG synthesis in seeds, seedlings, and leaves of Arabidopsis (Katavic et al, 1995;Lu et al, 2003;Slocombe et al, 2009), but pdat1 has not previously been shown to have a TAG phenotype. Recently, a third acyltransferase (DGAT3), with specificity toward polyunsaturated fatty acids, has also been shown to play a role in TAG synthesis in Arabidopsis seedlings (Hernández et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have shown that overexpression of DGAT1 and WRI1 individually leads to enhanced oil accumulation in vegetative tissues (BouvierNavé et al, 2000;Cernac and Benning, 2004) and also that a synergistic effect is achievable when they are expressed in combination (Vanhercke et al, 2013). WRI1 is a transcription factor that activates the expression of genes encoding multiple enzymes primarily of lower glycolysis and fatty acid synthesis (Cernac and Benning, 2004;Baud et al, 2007), and DGAT1 is the major enzyme that catalyzes the final committed step in TAG synthesis (Katavic et al, 1995;Zhang et al, 2009).…”

Section: Disruption Of Sdp1 In Lines Expressing Wri1 and Dgat1 Leads mentioning

confidence: 99%

“…The dgat1-1 and pdat1-1 mutants are described by Katavic et al (1995) and Zhang et al (2009), respectively. For experiments performed on medium, seeds were surface sterilized, plated on agar plates containing one-half-strength Murashige and Skoog salts (Sigma-Aldrich) with or without the addition of Suc, and imbibed in the dark for 4 d at 4°C.…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

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The SUGAR-DEPENDENT1 Lipase Limits Triacylglycerol Accumulation in Vegetative Tissues of Arabidopsis

et al. 2013

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There has been considerable interest recently in the prospect of engineering crops to produce triacylglycerol (TAG) in their vegetative tissues as a means to achieve a step change in oil yield. Here, we show that disruption of TAG hydrolysis in the Arabidopsis (Arabidopsis thaliana) lipase mutant sugar-dependent1 (sdp1) leads to a substantial accumulation of TAG in roots and stems but comparatively much lower TAG accumulation in leaves. TAG content in sdp1 roots increases with the age of the plant and can reach more than 1% of dry weight at maturity, a 50-fold increase over the wild type. TAG accumulation in sdp1 roots requires both ACYL-COENZYME A:DIACYLGLYCEROL ACYLTRANSFERASE1 (DGAT1) and PHOSPHATIDYLCHOLINE: DIACYLGLYCEROL ACYLTRANSFERASE1 and can also be strongly stimulated by the provision of exogenous sugar. In transgenic plants constitutively coexpressing WRINKLED1 and DGAT1, sdp1 also doubles the accumulation of TAG in roots, stems, and leaves, with levels ranging from 5% to 8% of dry weight. Finally, provision of 3% (w/v) exogenous Suc can further boost root TAG content in these transgenic plants to 17% of dry weight. This level of TAG is similar to seed tissues in many plant species and establishes the efficacy of an engineering strategy to produce oil in vegetative tissues that involves simultaneous manipulation of carbohydrate supply, fatty acid synthesis, TAG synthesis, and also TAG breakdown.

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Section: Tag Accumulation In Sdp1 Roots Is Greater Than In Several Otsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Disruption Of Sdp1 In Lines Expressing Wri1 and Dgat1 Leads mentioning

confidence: 99%

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

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The SUGAR-DEPENDENT1 Lipase Limits Triacylglycerol Accumulation in Vegetative Tissues of Arabidopsis

et al. 2013

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“…Much of the characterization of this gene to date has been focused on determining its effect on seed oil accumulation and the fatty acid composition of TAG in seed oil. To this end, seed-specific mutants have been analyzed (Katavic et al, 1995;Zou et al, 1999;Jako et al, 2001), and among the findings from these studies is the fact that dysfunctional DGAT1 protein results in a decrease in stored TAG and altered TAG fatty acid composition (Katavic et al, 1995). Overexpression of DGAT1 in Arabidopsis seeds engenders an increase in seed size and oil content, suggesting that DGAT catalyzes the ratelimiting step in TAG biosynthesis (Jako et al, 2001).…”

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confidence: 99%

A Role for Diacylglycerol Acyltransferase during Leaf Senescence

2002

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Lipid analysis of rosette leaves from Arabidopsis has revealed an accumulation of triacylglycerol (TAG) with advancing leaf senescence coincident with an increase in the abundance and size of plastoglobuli. The terminal step in the biosynthesis of TAG in Arabidopsis is catalyzed by diacylglycerol acyltransferase 1 (DGAT1; EC 2.3.1.20). When gel blots of RNA isolated from rosette leaves at various stages of development were probed with the Arabidopsis expressed sequence tag clone, E6B2T7, which has been annotated as DGAT1, a steep increase in DGAT1 transcript levels was evident in the senescing leaves coincident with the accumulation of TAG. The increase in DGAT1 transcript correlated temporally with enhanced levels of DGAT1 protein detected immunologically. Two lines of evidence indicated that the TAG of senescing leaves is synthesized in chloroplasts and sequesters fatty acids released from the catabolism of thylakoid galactolipids. First, TAG isolated from senescing leaves proved to be enriched in hexadecatrienoic acid (16:3) and linolenic acid (18:3), which are normally present in thylakoid galactolipids. Second, DGAT1 protein in senescing leaves was found to be associated with chloroplast membranes. These findings collectively indicate that diacylglycerol acyltransferase plays a role in senescence by sequestering fatty acids de-esterified from galactolipids into TAG. This would appear to be an intermediate step in the conversion of thylakoid fatty acids to phloem-mobile sucrose during leaf senescence.Diacylglycerol (DAG) acyltransferase (DGAT; EC 2.3.1.20) mediates the final acylation step in the synthesis of triacylglycerol (TAG). It is present in most plant organs, including leaves, petals, fruits, anthers, and developing seeds (Hobbs et al., 1999). In seeds, TAG is thought to be synthesized within the membranes of the endoplasmic reticulum and subsequently released into the cytosol in the form of oil bodies, which bleb from the cytoplasmic surface of the endoplasmic reticulum (Huang, 1992). The stored TAG is localized in the interior of the oil body, and the surfaces of oil bodies are coated with a monolayer of phospholipid associated with oleosin, the major protein of oil bodies. The acyl chains of the phospholipid monolayer are embedded in the TAG interior of the oil body. Oleosin is a structural protein that is thought to prevent coalescence of oil bodies during seed dehydration (Huang, 1996). That oil bodies originate from the endoplasmic reticulum is consistent with the finding that enzymes of TAG synthesis, including DGAT, are present in microsomal membrane fractions, which are known to contain vesicles of endoplasmic reticulum (Kwanyuen and Wilson, 1986). In addition, TAG can be synthesized in vitro in the presence of microsomes isolated from developing seeds (Lacey et al., 1999).Although TAG formation in seeds is believed to occur in the ER, there have been several reports indicating that purified chloroplast envelope membranes from leaves are also capable of synthesizing this storage lipid (Siebe...

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Production of Polyhydroxyalkanoates (PHAs) in Transgenic Plants

Poirier

Gruys

2002

Biopolymers Online

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Introduction Historical Perspective Synthesis of PHA in Plants Synthesis of Poly(3HB) in Arabidopsis thaliana Synthesis of Poly(3HB) in Other Plants Synthesis of Poly(3HB‐ co ‐3HV) Copolymer in Plants Synthesis of MCL‐PHA in Plants Novel Uses for PHA in Plants PHA to Modify Fiber Properties PHA as a Feed Supplement PHA as a Tool to Study Plant Metabolic Pathways Patents on PHA in plants Perspective on PHA Production in Agricultural Crops

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Alteration of Seed Fatty Acid Composition by an Ethyl Methanesulfonate-Induced Mutation in Arabidopsis thaliana Affecting Diacylglycerol Acyltransferase Activity

Cited by 254 publications

References 33 publications

The SUGAR-DEPENDENT1 Lipase Limits Triacylglycerol Accumulation in Vegetative Tissues of Arabidopsis

The SUGAR-DEPENDENT1 Lipase Limits Triacylglycerol Accumulation in Vegetative Tissues of Arabidopsis

A Role for Diacylglycerol Acyltransferase during Leaf Senescence

Production of Polyhydroxyalkanoates (PHAs) in Transgenic Plants

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