Alterations and Abnormal Mitosis of Wheat Chromosomes Induced by Wheat-Rye Monosomic Addition Lines

Fu, Shulan; Yang, Meiyin; Fei, Yun-Yan; Tan, Feiquan; Ren, Zhenglong; Yan, Bin; Zhang, Huaiyu; Tang, Zongxiang

doi:10.1371/journal.pone.0070483

Cited by 41 publications

(37 citation statements)

References 34 publications

(40 reference statements)

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“…On this account, there were minor differences among 4844-12, 5113, and 5114, which belong to 6 P I . In addition, we found that elimination of repetitive sequences on some chromosomes of ABD genomes was present in 4844-12 (unpublished results), and this situation could also be found in wheat–rye monosomic addition lines [72]. Elimination of DNA sequences might be the common form of rearrangements in addition line.…”

Section: Discussionmentioning

confidence: 67%

Genetic Rearrangements of Six Wheat–Agropyron cristatum 6P Addition Lines Revealed by Molecular Markers

Han

Bai

et al. 2014

PLoS ONE

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Agropyron cristatum (L.) Gaertn. (2n = 4x = 28, PPPP) not only is cultivated as pasture fodder but also could provide many desirable genes for wheat improvement. It is critical to obtain common wheat–A. cristatum alien disomic addition lines to locate the desired genes on the P genome chromosomes. Comparative analysis of the homoeologous relationships between the P genome chromosome and wheat genome chromosomes is a key step in transferring different desirable genes into common wheat and producing the desired alien translocation line while compensating for the loss of wheat chromatin. In this study, six common wheat–A. cristatum disomic addition lines were produced and analyzed by phenotypic examination, genomic in situ hybridization (GISH), SSR markers from the ABD genomes and STS markers from the P genome. Comparative maps, six in total, were generated and demonstrated that all six addition lines belonged to homoeologous group 6. However, chromosome 6P had undergone obvious rearrangements in different addition lines compared with the wheat chromosome, indicating that to obtain a genetic compensating alien translocation line, one should recombine alien chromosomal regions with homoeologous wheat chromosomes. Indeed, these addition lines were classified into four types based on the comparative mapping: 6PI, 6PII, 6PIII, and 6PIV. The different types of chromosome 6P possessed different desirable genes. For example, the 6PI type, containing three addition lines, carried genes conferring high numbers of kernels per spike and resistance to powdery mildew, important traits for wheat improvement. These results may prove valuable for promoting the development of conventional chromosome engineering techniques toward molecular chromosome engineering.

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Section: Discussionmentioning

confidence: 67%

Genetic Rearrangements of Six Wheat–Agropyron cristatum 6P Addition Lines Revealed by Molecular Markers

Han

Bai

et al. 2014

PLoS ONE

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“…These changes may trigger complicated interactions between the repair of DNA damage and the regulation of cell cycles. Another aspect of genome shock maybe also include parentage-biased patterns of gene expression and the abnormal upand down-regulation of offspring genes and abnormality in the very nascent hybrid genomes: e.g., 2nF 1 and 2nF 2 (59,60). We hypothesize that the alteration of expression of these genes drives polyploidization.…”

Section: Discussionmentioning

confidence: 97%

Genomic incompatibilities in the diploid and tetraploid offspring of the goldfish × common carp cross

Liu

Luo

Chai

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

126

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Polyploidy is much rarer in animals than in plants but it is not known why. The outcome of combining two genomes in vertebrates remains unpredictable, especially because polyploidization seldom shows positive effects and more often results in lethal consequences because viable gametes fail to form during meiosis. Fortunately, the goldfish (maternal) × common carp (paternal) hybrids have reproduced successfully up to generation 22, and this hybrid lineage permits an investigation into the genomics of hybridization and tetraploidization. The first two generations of these hybrids are diploids, and subsequent generations are tetraploids. Liver transcriptomes from four generations and their progenitors reveal chimeric genes (>9%) and mutations of orthologous genes. Characterizations of 18 randomly chosen genes from genomic DNA and cDNA confirm the chimera. Some of the chimeric and differentially expressed genes relate to mutagenesis, repair, and cancer-related pathways in 2nF 1 . Erroneous DNA excision between homologous parental genes may drive the high percentage of chimeric genes, or even more potential mechanisms may result in this phenomenon. Meanwhile, diploid offspring show paternal-biased expression, yet tetraploids show maternal-biased expression. These discoveries reveal that fast and unstable changes are mainly deleterious at the level of transcriptomes although some offspring still survive their genomic abnormalities. In addition, the synthetic effect of genome shock might have resulted in greatly reduced viability of 2nF 2 hybrid offspring. The goldfish × common carp hybrids constitute an ideal system for unveiling the consequences of intergenomic interactions in hybrid vertebrate genomes and their fertility.allopolyploidization | chimeric genes | transcriptomes | sequence validation | vertebrate P olyploidization is much rarer in vertebrates than in plants, and the reasons for this difference remain a mystery (1-3). Traditional explanations include barriers to sex determination, physiological and developmental constraints (especially nuclearcytoplasmic interactions and related factors) (2, 3), and genome shock or dramatic genomic restructuring (2-4). One type of polyploidization, allopolyploidization, involves the genomes of two species. Hybridization, accompanied by polyploidization, triggers vast genetic and genomic imbalances, including abnormal quadrivalent chromosomal groups, dosage imbalances, a high rate of DNA mutations and combinations, and other non-Mendelian phenomena (5-7). The effects of these imbalances are usually deleterious and are rarely advantageous. Imbalances in many plant crops determine the fate of the allopolyploid offspring. Genomic changes immediately follow allopolyploidization. Various and SignificanceWhy is polyploidization rarer in animals than in plants? This question remains unanswered due to the absence of a suitable system in animals for studying instantaneous polyploidization and the crucial changes that immediately follow hybridization. RNA-seq analyses discover exte...

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“…pHvG38, pSc119.2, and pDb12H were labeled with Taxas-red-5-dUTP (Invitrogen) by nick translation. Probe labeling and hybridization were as described by Fu et al [2013]. Microphotographs of C-banded and FISH chromosomes were taken with an Olympus BX-51 microscope equipped with a DP-70 CCD camera.…”

Section: C-banding and Fishmentioning

confidence: 99%

A Novel Wheat-<b><i>Dasypyrum breviaristatum </i></b>Substitution Line with Stripe Rust Resistance

Guang-rong¹,

Zhao²,

Li³

et al. 2014

Cytogenet Genome Res

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The introduction of genetic variation from wild and cultivated Triticeae species has been a long-standing approach for wheat improvement. Dasypyrumbreviaristatum species harbor novel and agronomically important genes for resistance against multi-fungal diseases. The development of new wheat-D. breviaristatum introgression lines offers chances for the identification of stripe rust resistance gene(s). A wheat line, D11-5, was selected from a cross between wheat line MY11 and wheat-D. breviaristatum partial amphiploid TDH-2. It was characterized by FISH and PCR-based molecular markers. Chromosome counting revealed that the D11-5 line shows a hexaploid set of 2n = 6x = 42 chromosomes. FISH analysis using the Dasypyrum repetitive sequence pDb12H as a probe demonstrated that D11-5 contained a pair of D. breviaristatum chromosomes, while FISH with wheat D-genomic repetitive sequences revealed that the chromosome 2D was absent in D11-5. The functional molecular markers confirmed that the introduced D. breviaristatum chromosomes belong to the homoeologous group 2, indicating that D11-5 was a 2V^b (2D) disomic substitution line. Field resistance showed that the introduced D. breviaristatum chromosomes 2V^b were responsible for the stripe rust resistance at the adult plant stage. FISH, C-banding, and PCR-based molecular marker analysis indicated that the chromosome 2V^b of D. breviaristatum was completely different from the chromosome 2V of D. villosum. The identified wheat-D. breviaristatum chromosome substitution line D11-5 may be applied to produce agronomically desirable stripe rust resistance germplasm.

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Alterations and Abnormal Mitosis of Wheat Chromosomes Induced by Wheat-Rye Monosomic Addition Lines

Cited by 41 publications

References 34 publications

Genetic Rearrangements of Six Wheat–Agropyron cristatum 6P Addition Lines Revealed by Molecular Markers

Genetic Rearrangements of Six Wheat–Agropyron cristatum 6P Addition Lines Revealed by Molecular Markers

Genomic incompatibilities in the diploid and tetraploid offspring of the goldfish × common carp cross

A Novel Wheat-<b><i>Dasypyrum breviaristatum </i></b>Substitution Line with Stripe Rust Resistance

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