Alterations and correlations of gut microbiota, fecal, and serum metabolome characteristics in a rat model of alcohol use disorder

Abstract

BackgroundGrowing evidence suggests the gut microbiota and metabolites in serum or fecal may play a key role in the process of alcohol use disorder (AUD). However, the correlations of gut microbiota and metabolites in both feces and serum in AUD subjects are not well understood.MethodsWe established a rat model of AUD by a chronic intermittent ethanol voluntary drinking procedure, then the AUD syndromes, the gut microbiota, metabolomic profiling in feces and serum of the rats were examined, and correlations between gut microbiota and metabolites were analyzed.ResultsEthanol intake preference increased and maintained at a high level in experimental rats. Anxiety-like behaviors was observed by open field test and elevated plus maze test after ethanol withdraw, indicating that the AUD rat model was successfully developed. The full length 16S rRNA gene sequencing showed AUD significantly changed the β-diversity of gut microbial communities, and significantly decreased the microbial diversity but did not distinctly impact the microbial richness. Microbiota composition significantly changed in AUD rats, such as the abundance of Romboutsia and Turicibacter were significantly increased, whereas uncultured_bacterium_o_Mollicutes_RF39 was decreased. In addition, the untargeted metabolome analysis revealed that many metabolites in both feces and serum were altered in the AUD rats, especially involved in sphingolipid metabolism and glycerophospholipid metabolism pathways. Finally, multiple correlations among AUD behavior, gut microbiota and co-changed metabolites were identified, and the metabolites were directly correlated with the gut microbiota and alcohol preference.ConclusionThe altered metabolites in feces and serum are important links between the gut microbiota dysbiosis and alcohol preference in AUD rats, and the altered gut microbiota and metabolites can be potentially new targets for treating AUD.

Sex-Specific Effect of Ethanol on Colon Content Lipidome in a Mice Model Using Nontargeted LC/MS

“…29 influenced by alcohol-induced gut dysbiosis. 30 In our results, the total PE levels were significantly increased in the EF group of both male and female mice (Figure 4), but the PG and HBMP levels were decreased specifically in the male EF group. A previous study in male mice fed ethanol for 5 weeks showed decreased PGs in the liver in the EF group compared with controls.…”

“…Moreover, GPs are the main integral constituents of biological membranes, which play a crucial role in cellular functions. Modulation of GPs is influenced by alcohol-induced gut dysbiosis . In our results, the total PE levels were significantly increased in the EF group of both male and female mice (Figure ), but the PG and HBMP levels were decreased specifically in the male EF group.…”

Physalis alkekengi L. Calyx Extract Alleviates Glycolipid Metabolic Disturbance and Inflammation by Modulating Gut Microbiota, Fecal Metabolites, and Glycolipid Metabolism Gene Expression in Obese Mice

“…The analysis mainly included thawing, grinding, extraction, vacuum drying of fecal samples, etc. The detailed steps are following the reference and our previous study [35,36] and in Supplementary Materials.…”

Angelica polysaccharides relieve blood glucose levels in diabetic KKAy mice possibly by modulating gut microbiota: an integrated gut microbiota and metabolism analysis

“…Screening criteria for differential metabolites were set as FC > 2, P < 0.05, and VIP > 1. These parameters align harmoniously with those utilized in analogous research endeavors [ 26 ]. The differential metabolites of significant KEGG pathway enrichment were calculated utilizing a hypergeometric distribution test.…”