Alterations in antioxidant metabolism in coffee leaves infected by Cercospora coffeicola

Abstract

Brown eye spot (BE) caused by Cercospora coffeicola is the main disease of coffee crop. A variation in symptoms of BE has been reported in the field, raising suspicion of occurrence of new species. However, information about coffee- C. coffeicola interaction is still limited. This research aimed to determine the difference between antioxidant metabolism of coffee plants cultivar Mundo Novo inoculated with a strain isolated from a common BE lesion (CML 2984) and a strain isolated from a black BE lesion (CML 2985). The enzyme activity of peroxidase (POX), catalase (CAT), superoxide dismutase (SOD), ascorbate peroxidase (APX) and phenylalanine ammonia lyase (PAL) were determined. Activities of POX, APX, and PAL increased in plants inoculated with both strains compared to non-inoculated plants at 12 and 24 hours post inoculation (hpi). CAT activity increased in inoculated plants with black BE strain at 24 hpi and both strains at 48 hpi. The SOD activity only increased in inoculated plants with both strains at 48 hpi. These results show that an elevated antioxidant response was observed when the plants were challenged with both strains of C. coffeicola. Both strains produced lesions of the common type, suggesting that other factors lead to the development of black BE lesion type under field conditions and further investigation is needed.

Identification of mung bean germplasm for resistance against Cercospora canescens and to study the association of biochemical parameters with defense mechanisms

“…This suggests a relevant role of these enzymes in the defense metabolism of the host plant. Andrade et al (2016) also have reported a similar finding in coffee (Coffea arabica L.) crops infected with C. caffeicola, where the activity of PAL, POX, and SOD was subsequently increased at 24 and 48 hai. This depicts an elevated antioxidant activity because of invasion and subsequent infection by the hemibiotrophic pathogen.…”

Comprehensive Review of Fungi on Coffee

“…Brown Eye Spot or Cercospora Blotch caused by Cercospora coffeicola, has been reported on coffee. Besides, Andrade et al [50] showed that isolates producing brown eye spot and black spot can also cause prompt alterations in the antioxidant metabolism of coffee leaves. Armillaria root rot caused by Armillaria sp., has been reported on coffee, and this disease leads to coffee plant rot and the eventual death of the plant.…”

Efficiency of Fungicide Application an Using an Unmanned Aerial Vehicle and Pneumatic Sprayer for Control of Hemileia vastatrix and Cercospora coffeicola in Mountain Coffee Crops

“…The methods commonly used for plant health protection in coffee crops include the use of backpack sprayers, atomizers, and hydro-pneumatic and pneumatic sprayers. The limitations of these options are scarcity of specialized workers, limited operational capacity, low application efficiency, and excessive application rates; these combined factors result in losses of product to the soil, and environmental and human contaminations [12,13].…”