Alterations in expression of basement membrane proteins during tumour progression in oral mucosa

Kannan, S.; Balaram, Prabha; Chandran, G. Jagadeesh; Pillai, M. Radhakrishna; Mathew, Babu; Nalinakumari, K.R.; Nair, Muralidharan

doi:10.1111/j.1365-2559.1994.tb00571.x

Cited by 41 publications

(33 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…21,22 In the present study, RCs, and DCs generally exhibited a linear and continuous expression of collagen IV, in agreement with the staining pattern described for normal tissues. On the other hand, KOTs and ameloblastomas presented a highly discontinuous thin linear staining pattern, with Յ50% extension of the BM in 80% and 100% of cases, respectively.…”

Section: Discussionsupporting

confidence: 91%

Comparative analysis of the immunohistochemical expression of collagen IV, MMP-9, and TIMP-2 in odontogenic cysts and tumors

Henriques

Vasconcelos

Galvão

et al. 2011

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, an

View full text Add to dashboard Cite

Objective. The aim of this study was to evaluate the immunohistochemical expression of collagen IV, matrix metalloproteinase (MMP) 9 and tissue inhibitor of MMP (TIMP) 2 in dentigerous cysts (DCs), radicular cysts (RCs), keratocystic odontogenic tumors (KOTs), and ameloblastomas. Study design. Twenty cases of DCs, 20 RCs, 20 KOTs, and 20 ameloblastomas were selected and analyzed by immunohistochemistry. Results. Most DCs and RCs showed continuous and Ͼ50% staining for collagen IV in the basement membrane of the epithelium, whereas predominantly discontinuous thin and Յ50% staining was observed in KOTs and ameloblastomas, with a significant difference in staining percentage (P Ͻ .001). MMP-9 was diffusely distributed and localized in both epithelial and mesenchymal cells of all of the lesions analyzed. The staining percentage was higher in the epithelium (P ϭ .058) and mesenchyme (P ϭ .005) of KOTs and ameloblastomas. Moreover, the distribution pattern, location, and percentage of expression of TIMP-2 were similar in the lesions studied, except for ameloblastoma, with a significant difference in staining percentage (P Ͻ .001).Conclusion. These results demonstrate that the interaction between collagen IV, MMP-9, and TIMP-2 is an important factor for the establishment of differences in the biologic behavior of the odontogenic cysts and tumors studied. Factors related to the epithelial and mesenchymal components participate in the regulation of the growth of odontogenic cystic lesions and tumors. 1 The altered expression of specific proteins of the extracellular matrix (ECM), associated with the exuberant presence of matrix metalloproteinases (MMPs) and the absence of expression of metalloproteinase inhibitors (TIMPs), may influence the behavior of these lesions. In the case of tumors, this situation contributes to the growth and higher aggressiveness of the tumor. 2 The odontogenic keratocyst, recently reclassified as keratocystic odontogenic tumor (KOT), 3 is known for its aggressive nature and high rate of recurrence, especially compared with other odontogenic cysts. 4 Ameloblastoma is a locally aggressive benign epithelial odontogenic tumor with a marked invasion potential that results in multiple recurrences after enucleation and curettage. 5 In contrast, dentigerous (DC) and radicular (RC) cysts show an indolent behavior and rarely recur after surgical removal. KOT presents a cystic structure similar to that of DC and RC, but its invasive and destructive growth is similar to that of ameloblastoma. 6 KOT, ameloblastoma, DC, and RC show distinct evolutions and biologic behaviors. In view of this fact, a growing number of studies have tried to identify

show abstract

Section: Discussionsupporting

confidence: 91%

Comparative analysis of the immunohistochemical expression of collagen IV, MMP-9, and TIMP-2 in odontogenic cysts and tumors

Henriques

Vasconcelos

Galvão

et al. 2011

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, an

View full text Add to dashboard Cite

show abstract

“…Previous studies confirmed that the structural integrity of the ECM-BM was closely associated with carcinoma formation, development, invasion and prognosis of the patient (40,41). In various carcinomas, the BM usually becomes thin and easily degrades, releasing collagen into the surrounding environment.…”

Section: Discussionmentioning

confidence: 75%

Non-invasive detection of nasopharyngeal carcinoma using saliva surface-enhanced Raman spectroscopy

Qiu

Huang

et al. 2015

Oncology Letters

View full text Add to dashboard Cite

Abstract. The present study evaluated the use of saliva surface-enhanced Raman spectroscopy (SERS) for the detection of non-invasive nasopharyngeal carcinoma (NPC). SERS measurements were taken from 62 saliva samples, of which 32 were from NPC patients and 30 from healthy volunteers. Notable biochemical Raman bands in the SERS spectra were tentatively assigned to various saliva components. The saliva SERS spectra obtained from the NPC patients and the healthy volunteers were also analyzed by multivariate statistical techniques based on principal component analysis and linear discriminant analysis (PCA-LDA). Significant differences were observed between the saliva SERS spectral intensities for NPC patients and healthy volunteers, particularly at 447, 496, 635, 729, 1134, 1270 and 1448 cm -1 , which primarily contained signals associated with proteins, nucleic acids, fatty acids, glycogen and collagen. The classification results based on the PCA-LDA method provided a relatively high diagnostic sensitivity of 86.7%, specificity of 81.3% and diagnostic accuracy of 83.9% for NPC identification. The results from the present study demonstrate that saliva SERS analysis used in conjunction with PCA-LDA diagnostic algorithms possesses a promising clinical application for the non-invasive detection of NPC.

show abstract

“…During neoplastic invasion, glycoprotein synthesis in the basal membrane is slower and disorganized, leading to permanent loss of continuity. 5 In recognition of this feature, our analysis of oral mucosal squamous cell carcinomas for assessing laminin expression was not done in areas with intense inflammation, to avoid distorting the results.…”

Section: Discussionmentioning

confidence: 99%

Expressão da laminina na membrana basal em carcinoma escamocelular oral

Souza

Silva

et al. 2007

Rev. Bras. Otorrinolaringol.

View full text Add to dashboard Cite

The basement membrane is a dynamic structure that undergoes quantitative and qualitative changes during the progression of squamous cell carcinoma, which is essencially important in tumoral invasion and metastasis. Aim: This study is aimed at investigating the behavior of the basement membrane in oral squamous cell carcinomas with different malignancy scores, which were obtained through the immunohistochemical expression of the laminin, a glycoprotein present in the basement membrane. Study design: History cross-sectional cohort. Material and method: Thirty-one cases of oral squamous cell carcinoma were subjected to histological grading of malignant tumors. The immunohistochemical expression of the laminin in lesions bearing different scores of malignancy was evaluated according to intensity and integrity, using the StreptavidinBiotin complex method. Results: We noticed significant differences in the media between intensity and continuity laminin expression in relation to different grades of malignancy. Conclusion: Different expressions of laminin, a glycoprotein present in basement membranes were evident in oral cell carcinomas within different grades of histological malignancy.

show abstract

Alterations in expression of basement membrane proteins during tumour progression in oral mucosa

Cited by 41 publications

References 24 publications

Comparative analysis of the immunohistochemical expression of collagen IV, MMP-9, and TIMP-2 in odontogenic cysts and tumors

Comparative analysis of the immunohistochemical expression of collagen IV, MMP-9, and TIMP-2 in odontogenic cysts and tumors

Non-invasive detection of nasopharyngeal carcinoma using saliva surface-enhanced Raman spectroscopy

Expressão da laminina na membrana basal em carcinoma escamocelular oral

Contact Info

Product

Resources

About