Alterations in extracellular substances during the biofilm development of Pseudomonas aeruginosa on aluminium plates

Abstract

The chemical moieties during bio®lm formation of Pseudomonas aeruginosa on aluminium plates were examined for a period of 17 days. The effect of¯uid shearing upon bio®lm formation has also been investigated. The Fourier transform infrared (FTIR) spectrum of the bio®lm taken on the ®fth day showed signi®cant differences compared with the spectrum of the unattached bacterial cells, indicating that structural changes or modi®cations of the cell envelope had taken place during the development of the bio®lm. Major changes were also observed in the spectrum during the subsequent development of the bio®lm from day 5 to day 17. The increasing intensity of a band corresponding to the symmetric stretching mode of the carboxyl group indicated interactions between the carboxyl group and the aluminium surface. Increased bacterial colonization was also observed at the air±water interface of the aluminium plates when compared with the middle and the bottom parts. Changes in FTIR spectra of the bio®lm at the bottom, at the middle, and at the air±water interface suggest that the mechanisms of bacterial attachment differed by a ±COO ± interaction at the air± water interface, and by both ±COO ± and NH 3 groups beneath the water surface.

Physicochemical analysis of initial adhesion and biofilm formation of Methanosarcina barkeri on polymer support material

“…The initial attachment of microbial cells to an abiotic surface is known to induce phenotypic and physiological changes in the cell, which can promote adhesion by the release of EPS [41]. This phenotypic cell change has been reported to occur earlier on hydrophobic and low surface free energy materials than on hydrophilic high surface free energy materials, and is a role of hydrophobicity [33].…”

Soil Chemical Insights Provided through Vibrational Spectroscopy

“…A number of studies have used FTIR to characterize and study interactions of specific bacterial components including extracellular polymeric substances (EPS) (Badireddy et al, 2008;Beech et al, 1999;Eboigbodin and Biggs, 2008;Chorover, 2004, 2006;Wingender et al, 2001), lipopolysaccharides (LPS) (Brandenburg, 1993;Brandenburg et al, 2001Brandenburg et al, , 1997Brandenburg and Seydel, 1990;Kamnev et al, 1999;Chorover, 2007, 2008), phospholipids (Brandenburg et al, 1999;Brandenburg and Seydel, 1986;Cagnasso et al, 2010;Hübner and Blume, 1998), and DNA (Brewer et al, 2002;Falk et al, 1963;Mao et al, 1994;Pershina et al, 2009;Tsuboi, 1961;Zhou and Li, 2004). In addition, ATR-FTIR has emerged as a powerful tool for studying biofilm formation, composition, and structure (Beech et al, 2000;Cheung et al, 2000;Schmitt and Flemming, 1999;Spath et al, 1998).…”

A review of visualization techniques of biocolloid transport processes at the pore scale under saturated and unsaturated conditions

“…A biofilm may include cells as well as exopolymeric substances that serve as a substrate modifier for a number of reasons (e.g., [22,17,47]). Different bacterial strains may exhibit differential attachment (e.g., [6]).…”