Alterations in intracellular calcium homeostasis and platelet aggregation induced by ethanol

Rosado, Juan A.; Núñez, Ana M.; Pariente, José A.; Salido, Ginés M.

doi:10.1016/j.bbrc.2006.01.056

Cited by 11 publications

(1 citation statement)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moderate EtOH consumption protects against the development of cardio- and cerebrovascular disease by increasing high-density lipoprotein levels or by decreasing platelet activation and clotting activity [29–31]. On the other hand, EtOH induces oxidative stress, generates H 2 O 2 , and oxidizes sulphydryl groups in human platelets [32]. In the present study rat and human platelets were treated with 50 mM (2.4 g/l) EtOH, which is a well-established experiment concentration [33] that reflects plasma EtOH levels of approximately 2‰.…”

Section: Discussionmentioning

confidence: 99%

Effects of ethanol on aggregation, serotonin release, and amyloid precursor protein processing in rat and human platelets

Ehrlich

Humpel

2013

Platelets

View full text Add to dashboard Cite

It is known that oxidative stress leads to amyloid precursor protein (APP) dysregulation in platelets. Ethanol (EtOH) is a vascular risk factor and induces oxidative stress. The aim of the present study was thus to investigate whether EtOH affects APP processing in rat and human platelets. Platelets were exposed to 50 mM EtOH with and without 2 mM calcium-chloride (CaCl₂) for 20 or 180 minutes at 37°C. Platelet aggregation, serotonin release and APP isoforms 130 and 106/110 kDa were analyzed. As a control, 100 mM H₂O₂ was tested in rat platelets. Our data show that EtOH alone did not affect any of the analyzed parameters, whereas CaCl₂ significantly increased aggregation of rat and human platelets. In addition, CaCl₂ alone enhanced serotonin release in rat platelets. EtOH counteracted CaCl₂-induced aggregation and serotonin release. In the presence of CaCl₂, EtOH reduced the 130 kDa APP isoform in rat and human platelets. In conclusion, this study shows that in the presence of CaCl₂, EtOH affects the platelet function and APP processing in rat and human platelets.

show abstract