Alterations in lipid peroxidation and antioxidant status in different types of intracranial tumors within their relative peritumoral tissues

Zengin, Emel; Atukeren, Pınar; Kökoğlu, Emine; Gümüştaş, Koray; Zengin, Umit

doi:10.1016/j.clineuro.2008.11.008

Cited by 35 publications

(38 citation statements)

References 46 publications

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“…Elevated oxidative stress can modify a number of cellular targets and cause cell damage, and the subsequent lack of repair has been considered responsible for carcinogenesis [20]. Cancer cells can be subject to increased and persistent oxidative stress due to elevated levels of intracellular ROS generation.…”

Section: Discussionmentioning

confidence: 99%

Chemopreventive potential of β-Sitosterol in experimental colon cancer model - an In vitro and In vivo study

Arul

Ignacimuthu²,

Paulraj³

et al. 2010

BMC Complement Altern Med

201

105

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BackgroundAsclepias curassavica Linn. is a traditional medicinal plant used by tribal people in the western ghats, India, to treat piles, gonorrhoea, roundworm infestation and abdominal tumours. We have determined the protective effect of β-sitosterol isolated from A. curassavica in colon cancer, using in vitro and in vivo models.MethodsThe active molecule was isolated, based upon bioassay guided fractionation, and identified as β-sitosterol on spectral evidence. The ability to induce apoptosis was determined by its in vitro antiradical activity, cytotoxic studies using human colon adenocarcinoma and normal monkey kidney cell lines, and the expression of β-catenin and proliferating cell nuclear antigen (PCNA) in human colon cancer cell lines (COLO 320 DM). The chemopreventive potential of β-sitosterol in colon carcinogenesis was assessed by injecting 1,2-dimethylhydrazine (DMH, 20 mg/kg b.w.) into male Wistar rats and supplementing this with β-sitosterol throughout the experimental period of 16 weeks at 5, 10, and 20 mg/kg b.w.Resultsβ-sitosterol induced significant dose-dependent growth inhibition of COLO 320 DM cells (IC50 266.2 μM), induced apoptosis by scavenging reactive oxygen species, and suppressed the expression of β-catenin and PCNA antigens in human colon cancer cells. β-sitosterol supplementation reduced the number of aberrant crypt and crypt multiplicity in DMH-initiated rats in a dose-dependent manner with no toxic effects.ConclusionWe found doses of 10-20 mg/kg b.w. β-sitosterol to be effective for future in vivo studies. β-sitosterol had chemopreventive potential by virtue of its radical quenching ability in vitro, with minimal toxicity to normal cells. It also attenuated β-catenin and PCNA expression, making it a potential anticancer drug for colon carcinogenesis.

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Section: Discussionmentioning

confidence: 99%

Chemopreventive potential of β-Sitosterol in experimental colon cancer model - an In vitro and In vivo study

Arul

Ignacimuthu²,

Paulraj³

et al. 2010

BMC Complement Altern Med

201

105

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“…Our results corroborate these observations. Decrease in TBARS and disturbed activities of enzymatic and non-enzymatic antioxidants were reported in oral tumor tissues (Zengin et al, 2009). At lower concentrations, ROS can induce mitogenic response (Vurusaner et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Anti-tumor Initiating Potential of Andrographolide in 7,12-dimethylbenz[a]anthracene Induced Hamster Buccal Pouch Carcinogenesis

Manoharan¹,

Singh²,

Suresh³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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The aim of the study was to investigate the chemopreventive potential of andrographolide in 7,12-dimethylbenz(a) anthracene (DMBA)-induced hamster buccal pouch carcinogenesis. Oral tumors developed in the buccal pouch of golden Syrian hamsters at a 100% incidence on painting with 0.5% DMBA in liquid paraffin three times a week for 14 weeks. Marked abnormalities in the status of detoxification enzymes, lipid perxodiation and antioxidants were noticed in hamsters treated with DMBA alone. Oral administration of andrographolide at a dose of 50 mg/ kg bw to hamsters treated with DMBA not only completely prevented the tumor formation but also restored the status of the above mentioned biomarkers. The present study thus demonstrates the chemopreventive potential of andrographolide in DMBA-induced hamster buccal pouch carcinogenesis, which is probably due to its antioxidant potential as well as modulating effect on xenobiotic metabolising enzymes during DMBA-induced oral carcinogenesis.

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“…Reactive oxygen species (ROS) are detectable in lyophilized tissue samples and may cause nucleic acid fragmentation [32]. Brain tumor cells may be especially susceptible to lipid peroxidation that generates reactive oxygen species [33–37]. Antioxidants, dessicants, oxygen absorbers, and other cell stabilizer additives are potential avenues of study.…”

Section: Discussionmentioning

confidence: 99%

Lyophilized brain tumor specimens can be used for histologic, nucleic acid, and protein analyses after 1 year of room temperature storage

et al. 2013

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Frozen tissue, a gold standard biospecimen, can yield well preserved nucleic acids and proteins after over a decade but is vulnerable to thawing and has substantial fiscal, spatial, and environmental costs. A long-term room temperature biospecimen storage alternative that preserves broad analytical utility can potentially empower tissue-based research. As there is scant data on the analytical utility of lyophilized brain tumor biospecimens, we evaluated lyophilized (freeze-dried) samples stored for 1 year at room temperature. Lyophilized tumor tissue processed into paraffin sections produced good histology. Yields of extracted DNA, RNA, and protein approximated those of frozen tissue. After 1 year, lyophilized samples yielded high molecular weight DNA that permitted copy number variation analysis, IDH 1 mutation detection, and MGMT promoter methylation PCR. A 27 % decrease in RIN scores over the 1 year suggests that RNA degradation was inhibited though incompletely. Nevertheless, RT-PCR studies on lyophilized tissue performed similarly to frozen tissue. In contrast to FFPE tissues where protein bands were absent or shifted to a lower molecular weight, lyophilized samples showed similar protein bands as frozen tissue on SDS-PAGE analysis. Lyophilized tissue performed similarly to frozen tissue for Western blots and enzyme activity assays. Immunohistochemistry of lyophilized tissue that were processed into FFPE blocks often required longer incubation times for staining than standard FFPE samples but generally provided robust antigen detection. This preliminary study suggests that lyophilization has promise for long-term room temperature storage while permitting varied tests; however, further work is required to better stabilize nucleic acids particularly RNA.

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Alterations in lipid peroxidation and antioxidant status in different types of intracranial tumors within their relative peritumoral tissues

Cited by 35 publications

References 46 publications

Chemopreventive potential of β-Sitosterol in experimental colon cancer model - an In vitro and In vivo study

Chemopreventive potential of β-Sitosterol in experimental colon cancer model - an In vitro and In vivo study

Anti-tumor Initiating Potential of Andrographolide in 7,12-dimethylbenz[a]anthracene Induced Hamster Buccal Pouch Carcinogenesis

Lyophilized brain tumor specimens can be used for histologic, nucleic acid, and protein analyses after 1 year of room temperature storage

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