Alterations in lipidome and metabolome profiles of Nannochloropsis salina in response to reduced culture temperature during sinusoidal temperature and light

Willette, Stephanie; Gill, Saba Shahid; Dungan, Barry; Schaub, Tanner; Jarvis, Jacqueline M.; Hilaire, Rolston St.; Holguín, F. Omar

doi:10.1016/j.algal.2018.03.001

Cited by 42 publications

(24 citation statements)

References 70 publications

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“…A signal-to-noise threshold of 10 was selected for peak-picking. Data were processed using in-house developed macros that align component signals across multiple samples and perform library matching based upon a user generated lipid library derived from LIPID MAPS [ 63 , 64 ]. Lipid assignment was performed based on mass and heteroatom class ( Table S4 ).…”

Section: Methodsmentioning

confidence: 99%

Suboptimal Temperature Acclimation Affects Kennedy Pathway Gene Expression, Lipidome and Metabolite Profile of Nannochloropsis salina during PUFA Enriched TAG Synthesis

et al. 2018

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In humans, dietary polyunsaturated fatty acids (PUFAs) are involved in therapeutic processes such as prevention and treatment of cardiovascular diseases, neuropsychiatric disorders, and dementia. We examined the physiology, PUFA accumulation and glycerol lipid biosynthesis in the marine microalga Nannochloropsis salina in response to constant suboptimal temperature (<20 °C). As expected, N. salina exhibited significantly reduced growth rate and photosynthetic activity compared to optimal cultivation temperature. Total fatty acid contents were not significantly elevated at reduced temperatures. Cultures grown at 5 °C had the highest quantity of eicosapentanoic acid (EPA) (C20:5n3) and the lowest growth rate. Additionally, we monitored broadband lipid composition to model the occurrence of metabolic alteration and remodeling for various lipid pools. We focused on triacylglycerol (TAG) with elevated PUFA content. TAGs with EPA at all three acyl positions were higher at a cultivation temperature of 15 °C. Furthermore, monogalactosyldiacylglycerol and digalactosyldiacylglycerol, which are polar lipids associated with chloroplast membranes, decreased with reduced cultivation temperatures. Moreover, gene expression analysis of key genes involved in Kennedy pathway for de novo TAG biosynthesis revealed bimodal variations in transcript level amongst the temperature treatments. Collectively, these results show that Nannochloropsis salina is a promising source of PUFA containing lipids.

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Section: Methodsmentioning

confidence: 99%

Suboptimal Temperature Acclimation Affects Kennedy Pathway Gene Expression, Lipidome and Metabolite Profile of Nannochloropsis salina during PUFA Enriched TAG Synthesis

et al. 2018

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“…Amino acids role and response to abiotic stress varies across algal strains. Elevated amino acid concentrations in response to stress have been observed in plants 55 and algae 12 , 15 , 42 , 56 and was observed in NREL 46B-D3 in response to both heat and particularly the cold stress. General amino acid accumulation is a mechanism to relieve oxidative stress, which can be caused by hypothermia and the resulting the generation of H 2 O 2 in plants as well as Chlamydomonas 57 – 59 .…”

Section: Discussionmentioning

confidence: 99%

“…During mass cultivation in open ponds, fluctuations in temperature can stress or temporarily distort metabolic network processes affecting growth, overall productivity, and biomass composition [6][7][8] . As reported for several taxa 9,10 , cold stress, either individually 11,12 or in some combination with another environmental stress 13 , reduces growth/productivity and can result in increased lipid that exceeds the effects of nitrogen starvation 14 including unsaturated fatty acids 11,12,15 that maintain membrane permeability and fluidity 15,16 . However, the underlying mechanisms that control and regulate these and other physiological responses and adaptation to temperature stress are largely unknown.…”

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confidence: 92%

A multi-omic characterization of temperature stress in a halotolerant Scenedesmus strain for algal biotechnology

et al. 2021

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Microalgae efficiently convert sunlight into lipids and carbohydrates, offering bio-based alternatives for energy and chemical production. Improving algal productivity and robustness against abiotic stress requires a systems level characterization enabled by functional genomics. Here, we characterize a halotolerant microalga Scenedesmus sp. NREL 46B-D3 demonstrating peak growth near 25 °C that reaches 30 g/m2/day and the highest biomass accumulation capacity post cell division reported to date for a halotolerant strain. Functional genomics analysis revealed that genes involved in lipid production, ion channels and antiporters are expanded and expressed. Exposure to temperature stress shifts fatty acid metabolism and increases amino acids synthesis. Co-expression analysis shows that many fatty acid biosynthesis genes are overexpressed with specific transcription factors under cold stress. These and other genes involved in the metabolic and regulatory response to temperature stress can be further explored for strain improvement.

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“…The species, strain and environmental conditions determine whether the given microalga accumulates lipids, carbohydrates or both, and whether the accumulation of these storage compounds happens simultaneously or at different time scales [94,236]. While N limitation is the most important method boosting microalgae lipid production, other environmental factors, like supply of minerals [238,239], temperature [95,240], light intensity [95,241], production under photosynthetic or heterotrophic growth conditions [242,243] and the growth phase of microalgae [244] can also affect the quantity and quality of microalgae lipids. The detailed description of complex effects of nutrient supply status and other environmental factors on microalgae composition are beyond the scope of this review, and the interested reader is referred to recent reviews [92,94,245] and references therein.…”

Section: Lipidsmentioning

confidence: 99%

Nutritional value of microalgae for ruminants and implications from microalgae production

Lamminen

2021

CABI Reviews

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Microalgae are a diverse group of microorganisms that are an interesting alternative feed resource for ruminant production. Microalgae species with high protein concentration and adequate amino acid (AA) composition can be used to substitute conventional protein feeds, whereas species with high carbohydrate or lipid concentration can be used to supply energy. Microalgal polyunsaturated acids and short-chain fatty acids have potential to improve the nutritive value of ruminant milk and meat for human consumption and mitigate enteric methane emissions. Microalgae composition is very plastid in comparison to conventional ruminant feeds and it can be influenced relatively easily by environmental conditions, such as nutrient supply. Microalgae also contain many compounds, especially carbohydrates and cell coverings, which are not usually found in ruminant feeds. Standard feed evaluation methods involving the use of crucibles or nylon bags (detergent fibre method, in vitro digestibility and in vivo rumen incubation) suit poorly to the analysis of microalgae with microscopic particle size. This paper attempts to give a general overview of the nutritive value (protein, lipids and carbohydrates) of microalgae for ruminant feeding applications and the possibilities to tailor microalgae composition for a certain ruminant feeding objectives. In addition, the key knowledge gaps related to the nutritive value of microalgae for ruminant nutrition are identified.

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Alterations in lipidome and metabolome profiles of Nannochloropsis salina in response to reduced culture temperature during sinusoidal temperature and light

Cited by 42 publications

References 70 publications

Suboptimal Temperature Acclimation Affects Kennedy Pathway Gene Expression, Lipidome and Metabolite Profile of Nannochloropsis salina during PUFA Enriched TAG Synthesis

Suboptimal Temperature Acclimation Affects Kennedy Pathway Gene Expression, Lipidome and Metabolite Profile of Nannochloropsis salina during PUFA Enriched TAG Synthesis

A multi-omic characterization of temperature stress in a halotolerant Scenedesmus strain for algal biotechnology

Nutritional value of microalgae for ruminants and implications from microalgae production

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