Alterations in molecular status of plasma fibronectin associated with aging of normal human individuals

Lemańska-Perek, Anna; Pupek, Małgorzata; Polańska, Bożena; Leszek, Jerzy; Kątnik-Prastowska, Iwona

doi:10.1016/j.clinbiochem.2013.03.008

Cited by 23 publications

(33 citation statements)

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“…11,12 In a previous study, we have observed the high molecular FN forms, following SDS-PAGE under reducing conditions, in the plasma of patients with neurodegenerative diseases, 13 and plasma and pleural effusions of some patients with lung cancer and lung inflammation, 14 and also of elderly individuals. 5 These FN forms were identified as the subunits of soluble supra-molecular plasma FN-fibrin complexes, which were easily revealed by SDS-agarose immunoblotting. 15 The present study was undertaken to determine whether the soluble supra-molecular FN-fibrin complexes occurred in the plasma of children suffering from RRI.…”

Section: Introductionmentioning

confidence: 99%

“…It is involved in a wide variety of cellular functions, including cell adhesion, migration, proliferation and differentiation. 4 FN, secreted by hepatocytes, circulates in blood at varying concentrations in children, 5 and is involved in blood clotting, thrombosis, and wound healing processes. 5,6 In the presence of endothelial injury, circulating FN enters the extravascular space and becomes incorporated into the ECM.…”

Section: Introductionmentioning

confidence: 99%

“…4 FN, secreted by hepatocytes, circulates in blood at varying concentrations in children, 5 and is involved in blood clotting, thrombosis, and wound healing processes. 5,6 In the presence of endothelial injury, circulating FN enters the extravascular space and becomes incorporated into the ECM. 4 The binding of plasma FN to cellular integrin receptors leads to stretching of FN, leading to the formation of a fiber multimeric network.…”

Section: Introductionmentioning

confidence: 99%

“…4,8 FN derived from different cell types displays high structural and functional heterogeneity, which may result primarily from alternative splicing of its premRNA and different degrees of post-translational Nand O-glycosylations. 9 Moreover, FN has an ability to form cross-linked complexes with, for example, FN fragments, 10 various extracellular matrix components, 8 and some blood plasma components such as fibrin and fibrinogen, 5 creating high-molecular FN forms. The FN forms with increased molecular masses were shown to be produced by carcinoma cell lines in vitro.…”

Section: Introductionmentioning

confidence: 99%

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Occurrence of soluble supra-molecular FN–fibrin complexes in the plasma of children with recurrent respiratory infection

et al. 2014

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Objectives: Fibronectin (FN) is able to bind fibrin and FN-fibrin complexes and is found in the plasma of some patients suffering from inflammatory disease. The present study was undertaken to determine whether soluble supra-molecular FN-fibrin complexes were present in the plasma of children with recurrent respiratory infections (RRI). Design and methods: The frequency of occurrence and relative amounts of the supra-molecular FN-fibrin forms, concentrations of immunoglobulins and numbers of natural killer cells (NK) were determined in the plasma of children with recurrent respiratory infections. The frequencies of these parameters were compared with their frequencies in the plasma of children with acute respiratory infections and plasma from healthy children. Results: SDS-agarose immunoblotting of patients' plasma revealed the presence of several additional FN-fibrin bands, with decreasing electrophoretic mobilities and increasing molecular masses of 750 kDa, 1000 kDa, 1300 kDa, 1600 kDa and 1900 kDa. Such FN-fibrin complexes occurred with higher frequency and in larger amounts in the plasma of children with RRI and acute infection than they did in plasma from normal children. Moreover, bands above 1000 kDa were absent in most young healthy individuals. The occurrence of FN-fibrin complexes did not correlate with either immunoglobulin concentrations, or with the number of NK cells. Conclusions: The occurrence of plasma supra-molecular FN-fibrin complexes is associated with acute and recurrent respiratory infections of children.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Occurrence of soluble supra-molecular FN–fibrin complexes in the plasma of children with recurrent respiratory infection

et al. 2014

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“…Na circulação sanguínea a concentração de FN é cerca de 300-400 mg / L (LEMAŃSKA-PEREK et al, 2013). Existem duas formas principais de fibronectina: a plasmática e a celular.…”

Section: Discussionunclassified

Caracterização do fator de elongação Tu (EF-Tu) de Leptospira: aspectos relacionados à colonização e evasão ao sistema complemento do hospedeiro

Wolff¹

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Evaluation of fibronectin 1 in one dried blood spot and in urine after rhGH treatment

Ferro

Ventura

Pérez‐Mañá

et al. 2016

Drug Testing and Analysis

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Since the appearance of recombinant human growth hormone (rhGH) in the 1980s, its expansion and acquisition through the black market has increased, so the detection of its abuse continues to be a challenge. New biomarkers that are more reliable and sensitive, allowing a larger detection window, are still needed. In this line, Fibronectin 1 (FN1) has been proposed as a potential genetic and protein biomarker of rhGH abuse in peripheral blood lymphocytes, serum, and plasma. However, logistic problems associated with current blood collection in sports drug testing point towards potential new alternative matrices that could be good candidates to be evaluated. Results obtained in this study showed high ELISA FN1 levels in one dried blood spot and in urine samples in ten healthy male volunteers treated with rhGH. Results showed that especially dried blood spots appear as a potential good matrix to detect rhGH abuse by means of FN1 biomarker. Copyright © 2016 John Wiley & Sons, Ltd.

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Alterations in molecular status of plasma fibronectin associated with aging of normal human individuals

Cited by 23 publications

References 47 publications

Occurrence of soluble supra-molecular FN–fibrin complexes in the plasma of children with recurrent respiratory infection

Occurrence of soluble supra-molecular FN–fibrin complexes in the plasma of children with recurrent respiratory infection

Caracterização do fator de elongação Tu (EF-Tu) de Leptospira: aspectos relacionados à colonização e evasão ao sistema complemento do hospedeiro

Evaluation of fibronectin 1 in one dried blood spot and in urine after rhGH treatment

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