Alterations in seedling vigour and antioxidant enzyme activities in Catharanthus roseus under seed priming with native diazotrophs

Abstract

An experiment was conducted on Catharanthus roseus to study the effect of seed treatments with native diazotrophs on its seedling growth and antioxidant enzyme activities. The treatments had significant influence on various seedling parameters. There is no significant influence on dry matter production with the diazotrophs, Azospirillum and Azotobacter. However, the vital seedling parameters such as germination percentage and vigour index were improved. Azotobacter treatment influenced maximum of 50% germination, whereas Azospirillum and Azotobacter were on par with C. roseus with respect to their vigour index. There was significant difference in the population of total diazotrophs. Azospirillum and Azotobacter between rhizosphere and non-rhizosphere soils of C. roseus had the same trend and were observed at various locations of the study. The activities of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POX) and catalase (CAT) were increased to a significant extent due to the treatment with diazotrophs.

Comparative proteomic analysis provides new insights into mulberry dwarf responses in mulberry (Morus alba L.)

“…Antioxidant enzyme extracts were prepared by grinding 0.5 g leaf material in 3 mL ice-cold 50 mM Tris buffer (pH 7.8), containing 1 mM EDTA-Na 2 and 7.5% w/v polyvinylpolypyrrolidone. The slurry was centrifuged at 10 000 Â g (41C) for 20 min, and the total soluble enzyme activities were determined as described [24].…”

Antioxidant potential and indole alkaloid profile variations with water deficits along different parts of two varieties of Catharanthus roseus

“…GSH is an ideal biochemical to protect plants against stress including oxidative stress, heavy metals and certain exogenous and endogenous organic chemicals. Glutathione takes part in the control of H 2 O 2 levels [31,32]. Glutathione-an antioxidant helped to withstand Dioscorea rotundata plants under paclobutrazol application [33].…”

Co-aggregation in Azospirillum brasilensense MTCC-125 with other PGPR strains: Effect of physical and chemical factors and stress endurance ability

“…Plant growth promoting rhizobacteria (PGPR) may promote growth directly either by fixation of atmospheric nitrogen or by solubilization of minerals such as phosphorus (Karthikeyan et al, 2007(Karthikeyan et al, , 2008a. This can promote growth indirectly by the production of plant growth regulators Klopper and Schroth, 1978).…”