Alterations in the glutathione metabolism could be implicated in the ischemia-induced small intestinal cell damage in horses

Marañón, Gonzalo; Manley, William; Cayado, Patricia; Garcı́a, Cruz; Muela, Mercedes Sánchez de la; Vara, Elena

doi:10.1186/1746-6148-5-10

Cited by 8 publications

(7 citation statements)

References 51 publications

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“…This leads to an increase in intracellular calcium levels, initiates anaerobic glycolysis, and activates xanthine oxidase and cell death [1]. In horses, it has been demonstrated that part of this damage after intestinal ischemia is due to alterations in glutathione and S-adenosyl methionine [2]. Recovery of sufficient perfusion may facilitate correction of these changes.…”

Section: Introductionmentioning

confidence: 99%

Assessment of effects of methylene blue on intestinal ischemia and reperfusion in a rabbit model: hemodynamic, histological and immunohistochemical study

et al. 2020

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Background: Intestinal ischemia-reperfusion (IR) is an important clinical occurrence seen in common diseases, such as gastric dilatation-volvulus in dogs or colic in horses. Limited data is available on the use of methylene blue in veterinary medicine for intestinal ischemia-reperfusion. The present study aimed to compare the hemodynamic, histopathological, and immunohistochemical effects of two doses of methylene blue in two rabbit model groups In one group, 5 mg/kg IV was administered, and in another, 20 mg/kg IV was administered following a constant rate infusion (CRI) of 2 mg/kg/h that lasted 6 h. All the groups, including a control group had intestinal ischemiareperfusion. Immunohistochemical analysis was performed using caspase-3. Results: During ischemia, hemodynamic depression with reduced perfusion and elevated lactate were observed. During reperfusion, methylene blue (MB) infusion generated an increase in cardiac output due to a positive chronotropic effect, an elevation of preload, and an intense positive inotropic effect. The changes in heart rate and blood pressure were significantly greater in the group in which methylene blue 5 mg/kg IV was administered (MB5) than in the group in which methylene blue 20 mg/kg IV dose was administered (MB20). In addition, lactate and stroke volume variations were significantly reduced, and vascular resistance was significantly elevated in the MB5 group compared with the control group and MB20 group. The MB5 group showed a significant decrease in the intensity of histopathological lesion scores in the intestines and a decrease in caspase-3 areas, in comparison with other groups. Conclusions: MB infusion produced improvements in hemodynamic parameters in rabbits subjected to intestinal IR, with increased cardiac output and blood pressure. An MB dosage of 5 mg/kg IV administered at a CRI of 2 mg/ kg/h exhibited the most protective effect against histopathological damage caused by intestinal ischemiareperfusion. Further studies with MB in clinical veterinary pathologies are recommended to fully evaluate these findings.

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Section: Introductionmentioning

confidence: 99%

Assessment of effects of methylene blue on intestinal ischemia and reperfusion in a rabbit model: hemodynamic, histological and immunohistochemical study

et al. 2020

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“…According to the findings of Marañón et al . [ 19 ], the ischemic intestine releases proinflammatory molecules into the portal and systemic circulation, including hydrogen peroxide, superoxide radicals, cytokines, and arachidonic acid metabolites, causing direct tissue damage and increasing oxidative stress biomarkers. Zuluaga et al demonstrated that 96 hr after inducing gastric mucosal injury in horses, the average plasma concentration of MDA increases [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

“…Most of the moralities due to colic are associated with circulatory collapse due to the absorption of bacterial lipopolysaccharide after a series of events such as ischemic gut injury and rapid degeneration of the intestinal barrier [ 13 ]. Intestinal ischemia as a consequence of colic may lead to the accumulation of harmful oxidative nitrogen and oxygen products [ 19 ]. Assessment of oxidative stress markers may be a useful therapeutic target and a prognostic indicator in several conditions in horses, such as orthopedic-related conditions, Cushing’s syndrome, endometritis, and gastrointestinal diseases [ 21 ].…”

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confidence: 99%

Association of the severity of colic in horses with oxidative stress biomarkers, acute-phase proteins, and certain trace elements

HAJIMOHAMMADI,

GHANE,

GHARI TEHRANI

et al. 2023

JES

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Sixty-one horses were enrolled in this study and divided into 3 different groups according to their severity of colic (heart rate, oral mucous membrane color, and abdominal distention): a strangulating colic (SC) group (n=21), non-strangulating colic (NC) group (n=20), and control group (n=20) consisting of randomly selected normal horses without signs of colic. The serum concentrations of haptoglobin, tumor necrosis factor-α (TNFα), nitric oxide (NO), malondialdehyde (MDA), zinc, iron, and copper were evaluated in all horses. The average concentration of TNFα in the SC group was higher than that in the control group (P<0.001). The TNFα concentration was higher in the NC group compared with the control group (P<0.001). Furthermore, the average concentration of TNFα tended to be higher in the SC group compared with the NC group (P=0.052). The average concentration of haptoglobin in the SC group was higher than that in the control group (P<0.001). The average concentration of NO was higher in the SC group compared with the NC group. (P=0.016) The average concentration of MDA was higher in the SC group compared with the control group (P=0.042). Furthermore, the concentration of MDA was higher in the SC group compared with the NC group (P=0.048). TNFα in horses with signs of colic may be a reliable indicator of prognosis and the severity of clinical signs. The haptoglobin concentration may be a useful marker in cases where animals are referred to clinicians a few days after the onset of colic. The concentrations of MDA and NO should be interpreted with caution.

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“…Thus, this suggests that the limiting level of RSNO, DNIC or B-DNIC in horse serum is 200–300 nM. It was shown that with colic of the gastrointestinal tract the level of the main cellular antioxidant glutathione decreases, which may lead to an increase in the role of hemoglobin in NO metabolism [ 39 ]. Thus, our data on the reduction of nitrite in serum may indicate a decrease in the both transport and regulatory forms of NO: RSNO, DNIC or B-DNIC in the serum of horses.…”

Section: Discussionmentioning

confidence: 99%

Estimation of Nitrite—Nitric Oxide Derivative—In Horses with Intestinal Colic by ESR Spectroscopy

Borunova

Tkachev

Иолчиев

et al. 2020

Veterinary Sciences

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Diseases of the gastrointestinal tract of horses are caused by many factors and have a complex pathogenesis. Developing effective methods of differential diagnostics is of high fundamental and applied importance. The pathogenesis of diseases of the digestive tract of horses accompanied by the development of inflammation and oxidative stress, can be associated with a lack of the nitrogen monoxide which controls many signaling pathways in the body. The level of the nitric oxide (NO) is involved in the regulation of the immune and nervous systems, the tone of all the blood vessels, and the courses of many pathological processes. The nitric oxide activates guanylate cyclase (sGC) and leads to vascular relaxation. The aim of this investigation was to study the metabolites of nitric oxide in horses suffered from intestinal diseases. The levels of nitric oxide in the blood serum of horses depending on their age and health state was studied. The concentration of nitrites in the blood serum of horses aged 6–25 years was 3.4 ± 4.2 μM, and in the young horses (1–5 years) the level of this indicator was 8.2 ± 5.4 μM. A sharp decrease in nitrite was observed in all the horses with intestinal diseases of 2 ± 0.9 μM, especially with tympanitic caecun of 0.6 ± 0.4 μM and with spasmodic colic of 1.8 ± 0.5 μM. The level of nitrosylhemoglobin HbNO in the blood of the diseased animals was higher than that in clinically healthy horses, regardless of age.

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Alterations in the glutathione metabolism could be implicated in the ischemia-induced small intestinal cell damage in horses

Cited by 8 publications

References 51 publications

Assessment of effects of methylene blue on intestinal ischemia and reperfusion in a rabbit model: hemodynamic, histological and immunohistochemical study

Assessment of effects of methylene blue on intestinal ischemia and reperfusion in a rabbit model: hemodynamic, histological and immunohistochemical study

Association of the severity of colic in horses with oxidative stress biomarkers, acute-phase proteins, and certain trace elements

Estimation of Nitrite—Nitric Oxide Derivative—In Horses with Intestinal Colic by ESR Spectroscopy

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