Alterations of 86Rb+ fluxes in poliovirus-infected HeLa cells and their dependence on virus replication

Schaefer, András; Geck, Peter; Zibirre, Reinhard; Kühne, J; Koch, Gebhard

doi:10.1016/0042-6822(84)90184-3

Cited by 10 publications

(2 citation statements)

References 24 publications

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“…This passage of molecules occurs in both directions: from the cell interior to the culture medium and vice versa. The alterations that take place in cytoplasmic concentrations of monovalent cations provoke membrane depolarization, finally leading to cell lysis [10,14,15].…”

Section: Late Membrane Changes Induced By Picornavirusesmentioning

confidence: 99%

Mechanisms of membrane permeabilization by picornavirus 2B viroporin

et al. 2003

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Cell infection by picornaviruses leads to membrane permeabilization. Recent evidence suggests the involvement of the non-structural protein 2B in this process. We have recently reported the detection of 2B porin-like activity in isolated membrane^protein systems that lack other cell components. According to data derived from these model membranes, four self-aggregated 2B monomers (i.e. tetramers) would be su⁄cient to permeabilize a single lipid vesicle, allowing the free di¡usion of solutes under ca. 1000 Da. Our ¢ndings also support a role for lipids in protein oligomerization and subsequent pore opening. The lipid dependence of these processes points to negatively charged cytofacial surfaces as 2B cell membrane targets.

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Section: Late Membrane Changes Induced By Picornavirusesmentioning

confidence: 99%

Mechanisms of membrane permeabilization by picornavirus 2B viroporin

et al. 2003

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“…As for Sindbis virus, cytolytic or fusogenic viruses of several other groups, including picornaviruses, induce alterations in intracellular cation concentrations (Alonso & Carrasco, 1981 ;Schaefer et al, 1984;Castrillo et al, 1987;Garry, 1989a, b;Garry & Waite, 1979;Carrasco & Esteban, 1982;Carrasco et aI., 1989;Cloyd et al, 1989;Cloyd & Lynn, 1991). Incubation of EMC virusinfected HeLa cells in medium containing a reduced concentration of NaC1 results in a reversal of the selective termination of host protein synthesis and an inhibition of the synthesis of EMC virus-specified proteins (Alonso & Carrasco, 1981).…”

mentioning

confidence: 99%

Sindbis virus-induced inhibition of protein synthesis is partially reversed by medium containing an elevated potassium concentration

Garry

1994

Journal of General Virology

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Infection of cultured vertebrate cells by Sindbis virus, an alphavirus, results in a reduction in the overall rate of protein synthesis and in selective termination (shutoff) of host-specified protein synthesis. The shutoff of host protein synthesis by Sindbis virus has been temporally correlated with a decrease in intracellular K + concentration (and an increase in intraceUular Na ÷ concentration) which occurs as a consequence of virusmediated inhibition of the plasma membrane-associated Na+/K + ATPase. Incubation of Sindbis virus-infected cells in medium containing an elevated concentration of K + resulted in an increase in the intracellular concentration of K ÷, an increase in the overall rate of protein synthesis, and in partial reversal of the virusinduced termination of cell-specified protein synthesis. These results suggest that the virus-induced decrease in intracellular K ÷ concentration is required for efficient shutoff of host protein synthesis by Sindbis virus.

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The Na⁺,K⁺,2Cl⁻‐cotransport system in HeLa cells: Aspects of its physiological regulation

Kort

Koch

1990

Journal Cellular Physiology

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We have previously reported on the biochemical properties of a Na+,K+,2Cl-cotransport system in HeLa cells and here we deal with aspects of its physiological regulation. Na+,K+,2Cl-cotransport in HeLa cells was studied by 86Rb+ influx and 86Rb+/22Na+ efflux measurements. The effects of rat atrial natriuretic peptide (ANP), isoproterenol, and amino acids on 86Rb+ flux, mediated by the bumetanide-sensitive Na+,K+,2Cl-cotransport system and the ouabain-sensitive Na+/K(+)-pump, were investigated. ANP reduced bumetanide-sensitive 86Rb+ influx under isotonic as well as under hypertonic conditions. Similar decrease of bumetanide-sensitive 86Rb+ influx was observed in the presence of 8-bromo-cGMP, while neither isoproterenol as a beta-receptor agonist nor 8-bromo-cAMP-could alter bumetanide-sensitive 86Rb+ influx. Furthermore, efflux of 86Rb+ and 22Na+ was greatly reduced in the presence of bumetanide and ANP. Together with our recent findings, showing functionally active, high affinity receptors for ANP on HeLa cells (Kort and Koch, Biochim. Biophys. Res. Commun. 168: 148-154, 1990), this study indicates that ANP participates in the regulation of the Na+,K+,2Cl-cotransport system in HeLa cells. Further measurements revealed that amino acids as present in the growth medium (Joklik's minimal essential medium) and the amino acid derivative alpha-methyl-aminoisobutyric acid (metAIB, 1 and 5 mM, respectively) also reduced Na+,K+,2Cl-cotransport-mediated 86Rb+ uptake and diminished the stimulatory effect of hypertonicity on the contransporter. In addition, the Na+/K(+)-pump was markedly stimulated in the presence of amino acids, while neither ANP and 8-Br-GMP nor isoproterenol and 8-Br-cAMP had a significant effect on the activity of the Na+/K(+)-pump.

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Alterations of 86Rb+ fluxes in poliovirus-infected HeLa cells and their dependence on virus replication

Cited by 10 publications

References 24 publications

Mechanisms of membrane permeabilization by picornavirus 2B viroporin

Mechanisms of membrane permeabilization by picornavirus 2B viroporin

Sindbis virus-induced inhibition of protein synthesis is partially reversed by medium containing an elevated potassium concentration

The Na⁺,K⁺,2Cl⁻‐cotransport system in HeLa cells: Aspects of its physiological regulation

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Alterations of 86Rb+ fluxes in poliovirus-infected HeLa cells and their dependence on virus replication

Cited by 10 publications

References 24 publications

Mechanisms of membrane permeabilization by picornavirus 2B viroporin

Mechanisms of membrane permeabilization by picornavirus 2B viroporin

Sindbis virus-induced inhibition of protein synthesis is partially reversed by medium containing an elevated potassium concentration

The Na+,K+,2Cl−‐cotransport system in HeLa cells: Aspects of its physiological regulation

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The Na⁺,K⁺,2Cl⁻‐cotransport system in HeLa cells: Aspects of its physiological regulation