2003
DOI: 10.1128/jb.185.24.7103-7110.2003
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Alterations of Cell Wall Structure and MetabolismAccompany Reduced Susceptibility to Vancomycin in an IsogenicSeries of Clinical Isolates of Staphylococcusaureus

Abstract: A series of isogenic methicillin-resistant Staphylococcus aureus isolates recovered from a bacteremic patient were shown to acquire gradually increasing levels of resistance to vancomycin during chemotherapy with the drug (K. Sieradzki, T. Leski, L. Borio, J. Dick, and A. Tomasz, J. Clin. Microbiol. 41:1687-1693, 2003). We compared properties of the earliest (parental) vancomycin-susceptible isolate, JH1 (MIC, 1 g/ml), to two late (progeny) isolates, JH9 and JH14 (vancomycin MIC, 8 g/ml). The resistant isolate… Show more

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Cited by 166 publications
(172 citation statements)
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References 23 publications
(42 reference statements)
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“…Increased resistance to vancomycin is associated with changes in bacterial morphology, physiology, and growth characteristics (8,19,27). In particular, increased resistance to vancomycin decreased the growth rate and cell size relative to isogenic strains of lower resistance (19).…”
Section: Resultsmentioning
confidence: 99%
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“…Increased resistance to vancomycin is associated with changes in bacterial morphology, physiology, and growth characteristics (8,19,27). In particular, increased resistance to vancomycin decreased the growth rate and cell size relative to isogenic strains of lower resistance (19).…”
Section: Resultsmentioning
confidence: 99%
“…Increased resistance to vancomycin is associated with changes in bacterial morphology, physiology, and growth characteristics, most notably, an increase in the thickness of the cell wall (8,11,21,27). The increased thickness of the cell wall correlates with an increase in genes involved in cell wall biosynthesis and regulation (18); hence, there is likely an increased demand for cell wall biosynthetic components N-acetyl-glucosamine and N-acetylmuramic acid.…”
Section: Discussionmentioning
confidence: 99%
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“…Such bacteria have been variously described as 'large multiseptate organisms' [151], 'pseudomulticellular bacteria' [60,152], and 'multicellular clusters' [153]. The term 'aggregates' is sometimes used also [154], but this is misleading because it implies the altered forms have arisen from cells coming together rather than cells failing to separate. Given their outward appearance as abnormally large or long cells, pseudomulticellular bacteria could potentially be mistaken for spheroplasts or other phenotypes unless transmission electron microscopy (TEM) is performed to detect the multiple septa.…”
Section: Pseudomulticellular Bacteriamentioning
confidence: 99%
“…However, it has an important role in antibiotic resistance, as it is essential for the expression of β-lactam resistance in community-acquired methicillin-resistant strains (25). Interestingly, inactivation of PBP4 has been reported in laboratory step mutants and clinical isolates with intermediate vancomycin resistance (27)(28)(29), which have decreased levels of PGN crosslinking. This suggests that modulation of the degree of crosslinking is important for resistance to different antibiotics.…”
mentioning
confidence: 99%