Alterations of CMP-NeuAc:Asialofetuin Sialyltransferase Activities in Human Colorectal Adenocarcinoma

Vázquez-Martín, Cristina; Gil‐Martín, Emilio; Fernández-Briera, Almudena

doi:10.1159/000066515

Cited by 4 publications

(4 citation statements)

References 19 publications

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“…The presence of both enzyme and product was similar to normal colon mucosa with the difference that for a complete overlap, deacetylation procedures must be applied. In line with this, a previous study has shown that normal and cancerous colon tissues have similar ST6GalNAc enzymatic activity (53). Interestingly enough, transitional tissues have been shown to display increased enzymatic activity when compared to normal mucosa and cancer (54).…”

Section: Discussionsupporting

confidence: 71%

ST6GalNAc-I controls expression of sialyl-Tn antigen in gastrointestinal tissues

Marcos

Bennett²,

Gomes³

et al. 2011

Front Biosci

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Section: Discussionsupporting

confidence: 71%

ST6GalNAc-I controls expression of sialyl-Tn antigen in gastrointestinal tissues

Marcos

Bennett²,

Gomes³

et al. 2011

Front Biosci

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“…Thus, the biosynthesis of these tumour sialylated antigens could be explained not only by variations in fucosyltransferase activities, such as a decrease in α1,2-fucosyltransferase or an increase in α1-3/4 fucosyltransferase, but also by increased activities in α2, 3-sialyltransferases. Some reports in the literature have described alterations in the glycosyltransferase expression and activity in several tumours, which presented increased sialylation in the form of SLe x and SLe a determinants, indicating that variations of specific glycosyltransferases may be helpful for tumour prognosis. An increased mRNA expression of several fucosyl and sialyltransferases has been reported in colorectal carcinomas [41], as well as differences in ST activity [45,46]. Gretschel et al [42] described that high levels of ST3Gal III and ST6Gal I could be correlated to secondary tumour recurrence in gastric cancers.…”

Section: Discussionmentioning

confidence: 99%

Role of sialyltransferases involved in the biosynthesis of Lewis antigens in human pancreatic tumour cells

et al. 2005

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The sialylated carbohydrate antigens, sialyl-Lewisx and sialyl-Lewisa, are expressed in pancreatic tumour cells and are related to their metastatic potential. While the action of the fucosyltransferases involved in the synthesis of these antigens has already been investigated, no studies have been carried out on the activity and expression of the alpha 2,3-sialyltransferases in pancreatic tumour cells. We describe the sialyltransferase (ST) activity, mRNA expression, and analysis of the cell carbohydrate structures in four human pancreatic adenocarcinoma cell lines of a wide range of neoplastic differentiation stages and in normal human pancreatic tissues. Total ST activity measured on asialofetuin, employing a CMP fluorescent sialic acid, varied among the pancreatic cell lines and could be correlated to the expression of their cell surface antigens. However, in some of the pancreatic cell lines, no relationship could be established with their ST3Gal III and IV mRNA expression. Human pancreatic tissues also showed ST expression and activity. However, it presented a much higher expression of neutral fucosylated structures than sialylated structures. In conclusion, ST activity levels in pancreatic cells could be correlated to their expression of sialylated epitopes, which indicates their involvement in the formation of the sialyl-Lewis antigens, in addition to fucosyltransferase activities.

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“…Expressions of STs are more often deregulated in cancer and its up-regulation might indicate activation of a specific signaling pathway [15,16]. Abnormally high levels of ST mRNA expression and total activities were found in tumor-bearing cells and sera of cancer patients [17][18][19]. ST6Gal.I is one of the most frequently up-regulated glycosyltransferases in human cancers [20].…”

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confidence: 99%

Tissue and serum α2-3- and α2-6-linkage specific sialylation changes in oral carcinogenesis

et al. 2007

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Increased sialylation of cell surface glycoconjugates is among the key molecular changes associated with malignant transformation and cancer progression. We investigated significance of linkage-specific sialylation changes in oral carcinogenesis. Tissue and serum levels of total sialic acid (TSA), linkage-specific sialyltransferases (ST) and sialoproteins were analyzed from patients with oral precancerous conditions (OPC) and oral cancer as well as the post-treatment follow-up blood samples of oral cancer patients. TSA levels were measured using a spectrophotometric method. The linkage-specific lectins, Sambusus nigra (SNA) and Maackia amurensis (MAM) detects alpha 2-6- and alpha 2-3-linked sialic acid, respectively, were used to analyze ST activity and sialoproteins. Malignant tissues showed significantly higher levels of TSA, reactivity of SNA and MAM, and alpha 2,3-ST activity compared to the adjacent normal tissues. alpha 2,6-ST was also higher in malignant tissues. Similarly, the marker levels were higher in precancerous tissues than their adjacent normal tissues. Serum levels of TSA, TSA/ total proteins, alpha 2-6-sialoproteins and alpha 2,6-ST were markedly increased in untreated oral cancer patients compared to the controls and OPC as well as responder (CR) patients. Serum levels of the markers were higher or comparable between untreated oral cancer patients and non-responders (NR). Serum levels of alpha 2-3-sialylation were elevated in non-responders compared with the responders. Further, the observed sialylation changes in tissue and serum were found to be associated with various clinicopathological features and disease progression. Thus, the data suggest potential utility of sialylation markers in early detection, prognostication and treatment monitoring of oral cancer.

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Alterations of CMP-NeuAc:Asialofetuin Sialyltransferase Activities in Human Colorectal Adenocarcinoma

Cited by 4 publications

References 19 publications

ST6GalNAc-I controls expression of sialyl-Tn antigen in gastrointestinal tissues

ST6GalNAc-I controls expression of sialyl-Tn antigen in gastrointestinal tissues

Role of sialyltransferases involved in the biosynthesis of Lewis antigens in human pancreatic tumour cells

Tissue and serum α2-3- and α2-6-linkage specific sialylation changes in oral carcinogenesis

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