Alterations of steroid receptor coactivator-1 (SRC-1) immunoreactivities in specific brain regions of young and middle-aged female Sprague–Dawley rats

Zhang, Dongmei; Guo, Qiang; Bian, Chen; Zhang, Jiqiang; Lin, Sen; Su, Bingyin

doi:10.1016/j.brainres.2011.01.024

Cited by 33 publications

(20 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As our previous reports, SRC‐1 immunopositive materials were predominantly localized in the nuclei of the pyramidal and granular cells (Bian et al,2011; Zhang et al,2011a, b). In the females, both IHC and Western blot showed that no significant changes of SRC‐1 one week after ovariectomy when compared with the sham control ( P > 0.05), significant decrease was detected at 2 weeks postsurgery ( P < 0.01).…”

Section: Resultssupporting

confidence: 85%

“…Several studies have reported the expression of SRC‐1 mRNA and/or protein in the hippocampus of mice (Bian et al,2011; Nishihara et al,2003) or rats (Meijer et al,2000; Ogawa et al,2001; Zhang et al,2011a, b). Higher SRC‐1 expression in the male hippocampus was reported under physiological conditions in the rats (Bousios et al,2001) and mice (Bian et al,2011).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Gonadectomy differentially regulates steroid receptor coactivator‐1 and synaptic proteins in the hippocampus of adult female and male C57BL/6 mice

Bian

Zhu

Yang

et al. 2012

Synapse

Self Cite

View full text Add to dashboard Cite

Hippocampus is one of the most important structures that mediates learning and memory, cognition, and mental behaviors and profoundly regulated by sex hormones in a sex-specific manner, but the mechanism of underlying sex differences regulation is still unclear. We have previously reported that in the male and female mice, steroid receptor coactivator-1 (SRC-1) and some key synaptic proteins share similar developmental profile in the hippocampus, but how circulating sex hormones affect hippocampal SRC-1 as well as these synaptic proteins remain unclear. In this study, we examined how gonad sex hormones regulate hippocampal SRC-1, synaptophysin, PSD-95, and AMPA receptor subtype GluR1 by using immunohistochemistry and Western blot. The results showed that in the female mice, ovariectomy affected hippocampal SRC-1 and GluR1 were only detected at 2 weeks post operation, then it recovered to sham level; synaptophysin was unaffected at any timepoint examined; significant decrease of PSD-95 was only detected at 4 weeks post operation. However, in the male hippocampus, SRC-1 and PSD-95 were decreased from one week and lasted to 4 weeks after orchidectomy, GluR1 decreased from 2 weeks after orchidectomy, but synaptophysin remained unchanged as in the females. Correlation analysis showed the profiles of SRC-1 were positively correlated with GluR1 of the females, PSD-95 and GluR1 of the males, respectively. The above results suggested a distinct regulatory mode between female and male gonad hormones in the regulation of hippocampal SRC-1 and synaptic proteins, which may be one of the mechanisms contributing to the dimorphism of hippocampus during development and ageing.

show abstract

Section: Resultssupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Gonadectomy differentially regulates steroid receptor coactivator‐1 and synaptic proteins in the hippocampus of adult female and male C57BL/6 mice

Bian

Zhu

Yang

et al. 2012

Synapse

Self Cite

View full text Add to dashboard Cite

show abstract

“…A c c e p t e d M a n u s c r i p t 4 regulation areas [27]. We also found that SRC-1 is regulated by development in both sexes and by orchidectomy [28][29] but not ovariectomy in the hippocampus of rats [29].…”

Section: Page 4 Of 23mentioning

confidence: 65%

Steroid receptor coactivator-1 mediates letrozole induced downregulation of postsynaptic protein PSD-95 in the hippocampus of adult female rats

Liu

Huangfu

Zhao

et al. 2015

The Journal of Steroid Biochemistry and Molecular Biology

Self Cite

View full text Add to dashboard Cite

“…SRC-1 is the coactivator for nuclear receptors [45]. It has been reported that SRC-1 has multiple potentials in the hippocampus such as neural development and neural stem cell differentiation [4,22,46,47]. SRC-1 knockout induced significant behavior deficiencies in a memory test [19], and it mediated LET regulation on hippocampal synaptic protein PSD-95 [15], indicating a role for this coactivator in the regulation of hippocampus function.…”

Section: Discussionmentioning

confidence: 99%

Letrozole regulates actin cytoskeleton polymerization dynamics in a SRC-1 dependent manner in the hippocampus of mice

Zhao

Zhang

et al. 2017

The Journal of Steroid Biochemistry and Molecular Biology

Self Cite

View full text Add to dashboard Cite

In the hippocampus, local estrogens (E) derived from testosterone that is catalyzed by aromatase play important roles in the regulation of hippocampal neural plasticity, but the underlying mechanisms remain unclear. The actin cytoskeleton contributes greatly to hippocampal synaptic plasticity; however, whether it is regulated by local E and the related mechanisms remain to be elucidated. In this study, we first examined the postnatal developmental profiles of hippocampal aromatase and specific proteins responsible for actin cytoskeleton dynamics. Then we used aromatase inhibitor letrozole (LET) to block local E synthesis and examined the changes of these proteins and steroid receptor coactivator-1 (SRC-1), the predominant coactivator for steroid nuclear receptors. Finally, SRC-1 specific RNA interference was used to examine the effects of SRC-1 on the expression of these actin remodeling proteins. The results showed a V-type profile for aromatase and increased profiles for actin cytoskeleton proteins in both male and female hippocampus without obvious sex differences. LET treatment dramatically decreased the F-actin/G-actin ratio, the expression of Rictor, phospho-AKT (ser473), Profilin-1, phospho-Cofilin (Ser3), and SRC-1 in a dose-dependent manner. In vitro studies demonstrated that LET induced downregulation of these proteins could be reversed by E, and E induced increase of these proteins were significantly suppressed by SRC-1 shRNA interference. These results for the first time clearly demonstrated that local E inhibition could induce aberrant actin polymerization; they also showed an important role of SRC-1 in the mediation of local E action on hippocampal synaptic plasticity by regulation of actin cytoskeleton dynamics.

show abstract

Alterations of steroid receptor coactivator-1 (SRC-1) immunoreactivities in specific brain regions of young and middle-aged female Sprague–Dawley rats

Cited by 33 publications

References 53 publications

Gonadectomy differentially regulates steroid receptor coactivator‐1 and synaptic proteins in the hippocampus of adult female and male C57BL/6 mice

Gonadectomy differentially regulates steroid receptor coactivator‐1 and synaptic proteins in the hippocampus of adult female and male C57BL/6 mice

Steroid receptor coactivator-1 mediates letrozole induced downregulation of postsynaptic protein PSD-95 in the hippocampus of adult female rats

Letrozole regulates actin cytoskeleton polymerization dynamics in a SRC-1 dependent manner in the hippocampus of mice

Contact Info

Product

Resources

About