Alterations of the basement membrane and connective tissue antigens in human metastatic lymph nodes

Burtin, P; Chavanel, G.; Foidart, Jean‐Michel; André, Janine A.

doi:10.1002/ijc.2910310608

Cited by 23 publications

(12 citation statements)

References 5 publications

(5 reference statements)

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“…Evidence from ultrastructural studies also indicates a marked EBM deficiency at this site, with associated evidence of increased invasive activity (Carr et al, 1986;Gabbert, 1985). In addition, we have found marked EBM deficiency at the parenchyma-adjacent periphery of lymph-node secondaries (noted by Burtin et al, 1983) and of liver secondaries. Very similar findings have been reported for cerebral metastases from a variety of carcinomas by McArdle et al (1984): they found marked EBM deficiency in regions of the tumour periphery that were adjacent to brain parenchyma, but not in regions adjacent to tumour stroma.…”

Section: Effects Of Local Tissue Environment On Turnourphenotypesupporting

confidence: 62%

The influence of local tissue environment on epithelial basement membrane continuity in colorectal carcinomas

Hewitt

Keeble

Powe

et al. 1994

Intl Journal of Cancer

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For colorectal carcinomas, there is evidence that marked discontinuity of the epithelial basement membrane (EBM) is associated with higher malignant potential. Since the metastatic process appears to be selective, more discontinuous EBMs might be expected in secondary rather than in primary tumours. To test this prediction, we examined a series of 60 cases of colorectal carcinoma for which samples of lymph-node or liver metastases were available. Sections were immunocytochemically stained for laminin, and the continuity of tumour EBM was then assessed by observational rating as well as by detailed morphometric analysis for a sample of cases. Contrary to the above prediction, we find that EBMs tend to be more continuous in secondary tumours than in corresponding primary tumours. These results could be explained by the possibility that local tissue environmental factors have a major influence on EBM continuity. Supporting evidence comes from our previous observation that EBM is very discontinuous at the advancing edge of primary colorectal carcinomas, where the tumour is adjacent to collagen-I-deficient stroma. Further evidence from the present study is that the EBM is extremely discontinuous at the interface between metastases and specialised parechymal tissue, but more continuous at the interface between metastases and stromal connective tissue. Since basement membranes affect the differentiation and behaviour of adjacent cells, these findings suggest that host tissue may influence invasive activity through their effects on EBM continuity.

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Section: Effects Of Local Tissue Environment On Turnourphenotypesupporting

confidence: 62%

The influence of local tissue environment on epithelial basement membrane continuity in colorectal carcinomas

Hewitt

Keeble

Powe

et al. 1994

Intl Journal of Cancer

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“…In addition, a generalized lack of matrix components similar to that observed in vitro is manifested in the absence of basement membranes normally detectable by immunohistochemical staining for laminin and type IV collagen (33)(34)(35). A strong correlation exists between the presence of residual basement membranes and the degree of morphological differentiation of a tumor, tumors with the lowest degree of differentiation having no detectable basement membranes.…”

Section: Malignant Cells Produce Fibronectin But Often Do Not Deposimentioning

confidence: 99%

Fibronectin in cell adhesion and invasion

1984

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Fibronectin plays a major role in the adhesion of many cell types. The extent of cell adhesion in vitro is related not only to the ability of the cells to interact with matrix-bound fibronectin, when it is present, but also to the synthesis or lack of synthesis of fibronectin by the cells, and to the lack of deposition of synthesized fibronectin into an insoluble matrix surrounding the cells. Many malignant cells, regardless of whether they synthesize subnormal or normal amounts of fibronectin, fail to deposit that fibronectin into a surrounding insoluble matrix. The lack of fibronectin around such cells appears to reflect a general absence of extracellular matrix since other matrix components, such as collagen, laminin, and heparan sulfate proteoglycan, are concomitantly missing. Cells that lack their own cell surface fibronectin due either to lack of deposition or to lack of synthesis can nevertheless adhere to insoluble fibronectin matrices elaborated by other cells. These cellular characteristics appear to be associated with cell migration in vivo during embryogenesis, and the same characteristics may enhance the invasive potential of malignant cells. The remarkable effects that fibronectin has on cellular adhesion and the association of lack of extracellular matrix components with poorly differentiated and highly metastatic tumors in vivo mandates that more be learned about the molecular and cellular details of the interactions of cells with their surrounding matrix. Important information concerning tumor invasion will parallel such an understanding and may eventually become the basis for therapeutic approaches.

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“…Coll-IV was completely lost around glandular cells in the nodes. However, the presence of BM constituents has been shown in other metastatic tumors [ 13,14,23]. Our objective was to determine whether the immunoperoxidase (IP) and immunoalkaline phosphatase (AP) techniques provided similar localization of type IV collagen in the BM of prostatic epithelium and stromal elements (smooth muscle, nerves, vessels) in normal prostate, benign prostatic hyperplasia (BPH), neoplastic human prostate, and regional pelvic lymph nodes and to relate their relationship with the degree of tumor differentiation (the Gleason grading system).…”

Section: Introductionmentioning

confidence: 99%

Localization of type IV collagen in the basement membranes of human prostate and lymph nodes by immunoperoxidase and immunoalkaline phosphatase

et al. 1991

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The object of these studies was to examine the localization of type IV collagen (Coll-IV) in the basement membranes (BM) of epithelial and stromal elements (smooth muscle, nerves, vessels) in normal, hyperplastic, and neoplastic (primary and metastatic) prostate. We also examined the relationship of Coll-IV distribution to the degree of tumor differentiation (Gleason grading system). We compared immunoperoxidase (IP) and immunoalkaline phosphatase (AP) techniques in these studies and in selected samples we also evaluated immunofluorescence (IF) localization of Coll-IV and the effects of tissue fixation and pepsin digestion. We found that IF localization of Coll-IV was intense in unfixed sections. IP and AP reactions were absent in fixed, paraffin-embedded sections but pepsin treatment yielded intense and uniform reaction products in these same preparations. Both the IP and AP techniques showed similar localization of Coll-IV in the BM of normal, hyperplastic, and well-differentiated tumor. In most of the higher-grade tumors Coll-IV localization was reduced and a similar pattern of distribution was observed after IP and AP techniques. However, in some high-grade tumors the IP technique showed good localization but AP did not, and vice versa. Such discrepancies were noted in the BM of the tumor cells, as well as in the BM of the stromal elements and in lymph nodes with metastatic tumor. Thus, our study shows decreased Coll-IV localization in higher-grade tumors and suggests that the use of a single technique (IP or AP) may exaggerate this apparent loss of Coll-IV BM components. The exact cause of these discrepancies is unknown but they must reflect variable losses in the ability of the tumor cells to form BM, degradation or decreased synthesis of BM components by high-grade tumors, or a combination of the above.

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Alterations of the basement membrane and connective tissue antigens in human metastatic lymph nodes

Cited by 23 publications

References 5 publications

The influence of local tissue environment on epithelial basement membrane continuity in colorectal carcinomas

The influence of local tissue environment on epithelial basement membrane continuity in colorectal carcinomas

Fibronectin in cell adhesion and invasion

Localization of type IV collagen in the basement membranes of human prostate and lymph nodes by immunoperoxidase and immunoalkaline phosphatase

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