Alterations of the cytoskeleton in human cells in space proved by life-cell imaging

Corydon, Thomas J.; Kopp, Sascha; Wehland, Markus; Braun, Markus; Schütte, Andreas; Mayer, T.; Hülsing, Thomas; Oltmann, Hergen; Schmitz, Burkhard; Hemmersbach, Ruth; Grimm, Daniela

doi:10.1038/srep20043

Cited by 109 publications

(135 citation statements)

References 64 publications

Supporting

Mentioning

123

Contrasting

Unclassified

Order By: Relevance

“…The corresponding protein constitutes an insulin receptor substrate [37], and it has been shown that it is involved in the regulation of lamellipodia formation via activation of the WAVE2 protein [38]. Of note, it has recently been observed, that the formation of filopodia and lamellipodia in thyroid cancer cells were affected by the conditions of a TEXUS sounding rocket flight, which also includes vibration during the take-off phase [39]. Some of the identified genes listed in Table 2, if already characterized, were involved in unrelated general metabolic processes in the cell such as regulation of transcription, oxidation and reduction processes, generation of precursor metabolites and energy, mRNA splicing or DNA-templated transcription.…”

Section: Discussionmentioning

confidence: 99%

Pathway Analysis Hints Towards Beneficial Effects of Long-Term Vibration on Human Chondrocytes

Lützenberg

Solano

Buken

et al. 2018

Cell Physiol Biochem

Self Cite

View full text Add to dashboard Cite

Background/Aims: Spaceflight negatively influences the function of cartilage tissue in vivo. In vitro human chondrocytes exhibit an altered gene expression of inflammation markers after a two-hour exposure to vibration. Little is known about the impact of long-term vibration on chondrocytes. Methods: Human cartilage cells were exposed for up to 24 h (VIB) on a specialised vibration platform (Vibraplex) simulating the vibration profile which occurs during parabolic flights and compared to static control conditions (CON). Afterwards, they were investigated by phase-contrast microscopy, rhodamine phalloidin staining, microarray analysis, qPCR and western blot analysis. Results: Morphological investigations revealed no changes between CON and VIB chondrocytes. F-Actin staining showed no alterations of the cytoskeleton in VIB compared with CON cells. DAPI and TUNEL staining did not identify apoptotic cells. ICAM-1 was elevated and vimentin, beta-tubulin and osteopontin proteins were significantly reduced in VIB compared to CON cells. qPCR of cytoskeletal genes, ITGB1, SOX3, SOX5, SOX9 did not reveal differential regulations. Microarray analysis detected 13 differentially expressed genes, mostly indicating unspecific stimulations. Pathway analyses demonstrated interactions of PSMD4 and CNOT7 with ICAM. Conclusions: Long-term vibration did not damage human chondrocytes in vitro. The reduction of osteopontin protein and the down-regulation of PSMD4 and TBX15 gene expression suggest that in vitro long-term vibration might even positively influence cultured chondrocytes.

show abstract

Section: Discussionmentioning

confidence: 99%

Pathway Analysis Hints Towards Beneficial Effects of Long-Term Vibration on Human Chondrocytes

Lützenberg

Solano

Buken

et al. 2018

Cell Physiol Biochem

Self Cite

View full text Add to dashboard Cite

show abstract

“…Gravitational alterations influence endothelial cell (EC) proliferation, differentiation, signaling, gene expression, surface adhesion molecules, extracellular matrix proteins expression, and cause significant changes in cytoskeletal polymers2345678910.…”

mentioning

confidence: 99%

Impact of Simulated Microgravity on Cytoskeleton and Viscoelastic Properties of Endothelial Cell

Janmaleki

Pachenari

Seyedpour

et al. 2016

Sci Rep

View full text Add to dashboard Cite

This study focused on the effects of simulated microgravity (s-μg) on mechanical properties, major cytoskeleton biopolymers, and morphology of endothelial cells (ECs). The structural and functional integrity of ECs are vital to regulate vascular homeostasis and prevent atherosclerosis. Furthermore, these highly gravity sensitive cells play a key role in pathogenesis of many diseases. In this research, impacts of s-μg on mechanical behavior of human umbilical vein endothelial cells were investigated by utilizing a three-dimensional random positioning machine (3D-RPM). Results revealed a considerable drop in cell stiffness and viscosity after 24 hrs of being subjected to weightlessness. Cortical rigidity experienced relatively immediate and significant decline comparing to the stiffness of whole cell body. The cells became rounded in morphology while western blot analysis showed reduction of the main cytoskeletal components. Moreover, fluorescence staining confirmed disorganization of both actin filaments and microtubules (MTs). The results were compared statistically among test and control groups and it was concluded that s-μg led to a significant alteration in mechanical behavior of ECs due to remodeling of cell cytoskeleton.

show abstract

“…Corydon et al 31 proved by life-cell-imaging that significant alterations of the cytoskeleton occurred during microgravity exposure.…”

Section: Discussionmentioning

confidence: 99%

Effects of altered gravity induced by clinorotation on the cholinesterase activity of the non-sentient model Paramecium primaurelia (Protozoa)

et al. 2018

View full text Add to dashboard Cite

Compounds known as chemical mediators, including acetylcholine, have been found not only in animals and humans, but also in living organisms, like protozoa, which lack nervous system. In Paramecium primaurelia has been described a cholinergic system, which is proven to play an important role in cell-cell interactions during its developmental cycle. In our work we investigated the effects of exposure to simulated microgravity (3D Random Positioning Machine, 56 rpm, 10 -6 g) on the cholinesterase activity of the eukaryote unicellular-organism alternative-model P. primaurelia. Our results show that the exposure of P. primaurelia to microgravity for 6 h, 24 h, 48 h affects the localization and the amount of cholinesterase activity compared to cells grown under Earth gravity conditions (1 g). However, these effects are transient since P. primaurelia restores its normal cholinesterase activity after 72 h under microgravity conditions, as well as cells exposed up to 72 h to microgravity and then placed under terrestrial gravity for 48 h.

show abstract

Alterations of the cytoskeleton in human cells in space proved by life-cell imaging

Cited by 109 publications

References 64 publications

Pathway Analysis Hints Towards Beneficial Effects of Long-Term Vibration on Human Chondrocytes

Pathway Analysis Hints Towards Beneficial Effects of Long-Term Vibration on Human Chondrocytes

Impact of Simulated Microgravity on Cytoskeleton and Viscoelastic Properties of Endothelial Cell

Effects of altered gravity induced by clinorotation on the cholinesterase activity of the non-sentient model Paramecium primaurelia (Protozoa)

Contact Info

Product

Resources

About