Alterations of the Gut Microbiota and Metabolomics Associated with the Different Growth Performances of Macrobrachium rosenbergii Families

Abstract

To investigate the key gut microbiota and metabolites associated with the growth performance of Macrobrachium rosenbergii families, 16S rRNA sequencing and LC–MS metabolomic methods were used. In this study, 90 M. rosenbergii families were bred to evaluate growth performance. After 92 days of culture, high (H), medium (M), and low (L) experimental groups representing three levels of growth performance, respectively, were collected according to the weight gain and specific growth rate of families. The composition of gut microbiota showed that the relative abundance of Firmicutes, Lachnospiraceae, Lactobacillus, and Blautia were much higher in Group H than those in M and L groups. Meanwhile, compared to the M and L groups, Group H had significantly higher levels of spermidine, adenosine, and creatinine, and lower levels of L-citrulline. Correlation analysis showed that the abundances of Lactobacillus and Blautia were positively correlated with the levels of alpha-ketoglutaric acid and L-arginine. The abundance of Blautia was also positively correlated with the levels of adenosine, taurine, and spermidine. Notably, lots of metabolites related to the metabolism and biosynthesis of arginine, taurine, hypotaurine, and fatty acid were upregulated in Group H. This study contributes to figuring out the landscape of the gut microbiota and metabolites associated with prawn growth performance and provides a basis for selective breeding.

Canaries’ Microbiota: The Gut Bacterial Communities along One Female Reproductive Cycle

“…Vermiphilaceae family has been mentioned so far in studies investigating the gut microbiota of lizards ( Sceloporus spp. ), giant river prawn Macrobrachium rosenbergii , and ascidian ( Halocynthia roretzi ), which was put in relation to age, growth rate and season respectively [ 70 , 71 , 72 , 73 ]. Nevertheless, little is known about its role in the gut bacterial microbiota dynamics and its ecology in living host communities.…”

Comparative Transcriptome Analysis of the Hepatopancreas from Macrobrachium rosenbergii Exposed to the Heavy Metal Copper

“…Macrobrachium rosenbergii is primarily distributed in inland freshwater areas. Because of its short culture cycle, high nutritional value, and other advantages, it has become an important economic species in China [12]. In addition, M. rosenbergii has always been considered an important model organism with relevance for many research fields such as ecology [13], physiology [14], and toxicology [15].…”

Comparison of the gut microbiota and untargeted gut tissue metabolome of Chinese mitten crabs (Eriocheir sinensis) with different shell colors

“…Therefore, many studies have focused on E. sinensis metabolome variations related to food, disease, and the environment ( Chen et al, 2021b ; Guo et al, 2022 ; Jiang et al, 2022 ). In aquatic animals, integrative analysis of the microbiota and metabolome has been utilized to investigate alterations in the physiological status of hosts related to differences in nutrition intake ( Chen et al, 2021b ), habitats ( Chen et al, 2021a ), growth performance ( Uengwetwanit et al, 2020 ; Lan et al, 2023 ), and aquaculture systems ( Ding et al, 2022 ; Yokoyama et al, 2022 ).To our knowledge, there has been no metabolomic study related to the different shell colors of E. sinensis thus far. In this study, we investigated the gut microbiota and metabolomes of the gut tissue of juvenile RH and GH crabs.…”