Alterations to the protein profile of bladder carcinoma cell lines induced by plant extract MINA‐05 in vitro

Abstract

Bladder cancer (BLCa) is a severe urological cancer of both men and women that commonly recurs and once invasive, is difficult to treat. MINA-05 (CK Life Sciences Int'l, Hong Kong) is a derivative of complex botanical extracts, shown to reduce cellular proliferation of bladder and prostate carcinomas. We tested the effects of MINA-05 against human BLCa cell sublines, B8, B8-RSP-GCK, B8-RSP-LN and C3, from a transitional cell carcinoma, grade IV, to determine the molecular targets of treatment by observing the cellular protein profile. Cells were acclimatised for 48 h then treated for 72 h with concentrations of MINA-05 reflecting 1/2 IC(50), IC(50) and 2 x IC(50) (n = 3) or with vehicle, (0.5% DMSO). Dose-dependant changes in protein abundance were detected and characterised using 2-dimensional electrophoresis and MS. We identified 10 proteins that underwent changes in abundance, pI and/or molecular mass in response to treatment. MINA-05 was shown to influence proteins across numerous functional classes including cytoskeletal proteins, energy metabolism proteins, protein degradation proteins and tumour suppressors, suggesting a global impact on these cell lines. This study implies that the ability of MINA-05 to retard cellular proliferation is attributed to its ability to alter cell cycling, metabolism, protein degradation and the cancer cell environment.

Evaluation of Cytotoxicity and Anticarcinogenic Potential of Mentha Leaf Extracts

“…41 Studies in cancerous cells have shown that bioactive extracts of plant inhibit cell proliferation through alterations in cell cycling, energy metabolism proteins, and ultimately the cancer cell environment. 42,43 Concomitantly, in the present study, with the increase in the duration of exposure, there was a reduction in cell growth with apparent morphological changes in extracts treated HT-29, Jurkat, T24, MIAPaCa-2, MCF-7, and HeLa cells. Their growth was noticeable in a more disperse manner with progressive accumulation of b-gal stain after attaining senescence at 48 hours (Figure 5).…”

Proteomic analysis reveals cellular pathways regulating carbohydrate metabolism that are modulated in primary human skeletal muscle culture due to treatment with bioactives from Artemisia dracunculus L.

“…Differential global proteomic technologies provide a broad signature of changes in protein levels which allow identification of key pathways and mechanisms responsible for complex biological effects [8, 9]. Despite advancements in mass spectrometry based proteomic techniques to understand biological processes at the molecular level, only a limited number of studies have used proteomics to study mechanisms by which botanicals induce biological effects [10–13]. …”

Sensitive Time-Gated Immunoluminescence Detection of Prostate Cancer Cells Using a TEGylated Europium Ligand

“…The five different immunoconjugates of primary antibody (MIL38-BHHBTEGSB x , x = 7, 16,27,34,40) were tested in immuno-staining of cancer cell lines, using DU145 cells as positive control (GPC1+) and C3 bladder cancer cell line that does not express MIL38 antigen (GPC1-), 38,39 as negative control. The stained cells were inspected under TGL and conventional epifluorescence mode using a DAPI filter set.…”