2011
DOI: 10.1002/pro.611
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Altered architecture of substrate binding region defines the unique specificity of UDP‐GalNAc 4‐epimerases

Abstract: UDP-hexose 4-epimerases play a pivotal role in lipopolysaccharide (LPS) biosynthesis and Leloir pathway. These epimerases are classified into three groups based on whether they recognize nonacetylated UDP-hexoses (Group 1), both N-acetylated and nonacetylated UDPhexoses (Group 2) or only N-acetylated UDP-hexoses (Group 3). Although the catalysis has been investigated extensively, yet a definitive model rationalizing the substrate specificity of all the three groups on a common platform is largely lacking. In t… Show more

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Cited by 15 publications
(23 citation statements)
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“…57 The availability of a second group 3 epimerase, the WbgU from Pleisomonas shigelloides, and its structure provided more information on the substrate specificity of these enzymes. 16 Despite the relatively low sequence identity among all three groups, the similarity of the enzymes' tertiary structures is striking with an overall rmsd of the multiple structure alignment being 1.08 Å and variation being most pronounced at the C-terminal end. The model of the '297e308 belt' was proposed to determine substrate specificity in group 3 members.…”
Section: Non-acetylated Versus N-acetylated Udp-hexosesmentioning
confidence: 97%
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“…57 The availability of a second group 3 epimerase, the WbgU from Pleisomonas shigelloides, and its structure provided more information on the substrate specificity of these enzymes. 16 Despite the relatively low sequence identity among all three groups, the similarity of the enzymes' tertiary structures is striking with an overall rmsd of the multiple structure alignment being 1.08 Å and variation being most pronounced at the C-terminal end. The model of the '297e308 belt' was proposed to determine substrate specificity in group 3 members.…”
Section: Non-acetylated Versus N-acetylated Udp-hexosesmentioning
confidence: 97%
“…As a result, the S306Y mutation, which allows a switch from group 2 to group 1, forms steric clashes between the group 3 epimerases and their substrates, which results in the observed loss of activity. 16 The importance and flexibility of the hydrogen bond network was evidenced by multiple single mutants as well as their combined double, triple and quadruple mutants. 58 …”
Section: Non-acetylated Versus N-acetylated Udp-hexosesmentioning
confidence: 99%
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