Altered Contractile Responsiveness in Ileal Longitudinal Muscle After Total Gastrectomy in a Rat Model

Abstract

These data indicate that the contractile response to various agents is altered after total gastrectomy and that receptor-mediated mechanisms may cause these changes.

Protective Effects of L‐Arginine on Rat Terminal Ileum Subjected to Ischemia/Reperfusion

“…That the p D 2 values in all of the groups were found to be statistically unchanged, however, suggests that I/R do not alter acetylcholine‐receptor interaction. Therefore, the reduced E max value in I/R may be dependent partly on the change in the regulation of postreceptor processes (ie, excitation‐contraction coupling) (18,19). Administration of L‐arginine has improved the reduced contractile responses and returned them to sham‐control levels, whereas treatment with L‐NAME has no ameliorating effect on ileal contractility.…”

“…Isometric force was monitored by external force displacement transducer (FDA-10A, Commat I ˙letisim Co, Ankara) using MP 30 software (MP30 Biopac Systems Inc, Santa Barbara, CA). To evaluate the effects of I/R and the drugs on response to acetylcholine, the maximal contractile response (E max , as gram contraction) and pD 2 values (ie, the negative logarithm of the concentration for the half-maximal response, ED 50 ) (18,19) were calculated by Graphpad Prism Software version 3.0 (San Diego, CA). Samples of full-thickness segments that were to be analyzed for levels of GSH and MDA were frozen immediately after the reperfusion period and stored at À408C.…”