Altered Energy Metabolism, Mitochondrial Dysfunction, and Redox Imbalance Influencing Reproductive Performance in Granulosa Cells and Oocyte During Aging

Kobayashi, Hiroshi; Yoshimoto, Chiharu; Matsubara, Sho; Shigetomi, Hiroshi; Imanaka, Shogo

doi:10.1007/s43032-023-01394-7

Cited by 11 publications

(1 citation statement)

References 86 publications

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“…A recent study indicates that reduced mitophagy activators could lead to the accumulation of dysfunctional mitochondria in mouse oocytes, halting meiosis progression [ 32 ]. In that regard, dysfunctional mitochondria and energy metabolism have been associated with an imbalance of reactive oxygen species and their scavenging system in aged oocytes and granulosa cells, thereby resulting in age-related decline in fertility [ 33 ]. Perhaps central mitochondrial clustering is associated with altered oocyte metabolism due to maternal aging, resulting in lower ATP generation.…”

Section: Discussionmentioning

confidence: 99%

Effect of maternal age on ATP content and distribution of mitochondria in bovine oocytes

Dadarwal,

Pfeifer,

Cervantes

et al. 2024

PLoS ONE

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Our objective was to understand how maternal age influences the mitochondrial population and ATP content of in vivo matured bovine oocytes. We hypothesized that in vivo matured oocytes from older cows would have altered mitochondrial number and distribution patterns and lower cytoplasmic ATP content compared to the oocytes obtained from younger cows. Follicles ≥5mm were ablated in old cows (13 to 22 yrs, Old Group, n = 7) and their younger daughters (4 to 10 years old, Young Group; n = 7) to induce the emergence of a new follicular wave. Cows were treated twice daily with eight doses of FSH starting 24 hr after ablation (Day 0, day of wave emergence). Prostaglandin F2alpha (PGF) was given on Days 3 and 3.5, LH on Day 4.5, and cumulus-oocyte-complexes were collected 18–20 hours post-LH by ultrasound-guided follicular aspiration. Oocytes were either processed for staining with MitoTracker Deep Red FM or for ATP assay. Stained oocytes were imaged with a Zeiss LSM 710 confocal microscope, and mitochondria were segmented in the oocyte volume sets using Imaris Pro 7.4. In vivo matured oocytes obtained from old cows were similar in morphological grades to those from young cows. However, the oocytes of COC from older cows had 23% less intracellular ATP (27.4±1.9 vs 35.7±2.2 pmol per oocyte, P = 0.01) than those of young cows. Furthermore, the average volume of individual mitochondria, indicated by the number of image voxels, was greater (P<0.05) in oocytes from older cows than in those from younger cows. Oocytes from older cows also tended to have a greater number of mitochondrial clusters (P = 0.06) and an increased number of clusters in the central region of the oocytes (P = 0.04) compared to those from younger cows. In conclusion, our study demonstrated that maternal age was associated with a decrease in the cytoplasmic ATP content of in vivo mature oocytes and an altered distribution of mitochondrial structures. These findings suggest that maternal age may negatively influence the developmental competence of oocytes from older cows.

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Section: Discussionmentioning

confidence: 99%