Altered Eosinophil Proteome in a Patient with Hypereosinophilia from Acute Fascioliasis

Straub, C.; Burnham, Jason P.; White, A. Clinton; Pazdrak, Konrad; Sánchez, Cesar; Watanabe, Luis Carlos; Kurosky, Alexander; Montes, Martín

doi:10.1128/cvi.05373-11

Cited by 8 publications

(9 citation statements)

References 13 publications

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“…In dengue virus, P4HB was linked to a reduction of β1 and β3 integrins allowing for the entry of the virus (68) and in MA104 cells, thiol blockers, and P4HB inhibitors decreased the entry of rotavirus (66). The other recurring human protein is GANAB, which is related to the alteration of eosinophil proteome (69). Strikingly, eosinophils are important mediators of allergies, asthma, and adverse drug reactions, and are also related to the host defense mechanisms against helminth infections, which are characterized by eosinophilia (70–72).…”

Section: Discussionmentioning

confidence: 99%

Analysis of Predicted Host–Parasite Interactomes Reveals Commonalities and Specificities Related to Parasitic Lifestyle and Tissues Tropism

et al. 2019

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The study of molecular host–parasite interactions is essential to understand parasitic infection and adaptation within the host system. As well, prevention and treatment of infectious diseases require a clear understanding of the molecular crosstalk between parasites and their hosts. Yet, large-scale experimental identification of host–parasite molecular interactions remains challenging, and the use of computational predictions becomes then necessary. Here, we propose a computational integrative approach to predict host—parasite protein—protein interaction (PPI) networks resulting from the human infection by 15 different eukaryotic parasites. We used an orthology-based approach to transfer high-confidence intraspecies interactions obtained from the STRING database to the corresponding interspecies homolog protein pairs in the host–parasite system. Our approach uses either the parasites predicted secretome and membrane proteins, or only the secretome, depending on whether they are uni- or multi-cellular, respectively, to reduce the number of false predictions. Moreover, the host proteome is filtered for proteins expressed in selected cellular localizations and tissues supporting the parasite growth. We evaluated the inferred interactions by analyzing the enriched biological processes and pathways in the predicted networks and their association with known parasitic invasion and evasion mechanisms. The resulting PPI networks were compared across parasites to identify common mechanisms that may define a global pathogenic hallmark. We also provided a study case focusing on a closer examination of the human– S. mansoni predicted interactome, detecting central proteins that have relevant roles in the human– S. mansoni network, and identifying tissue-specific interactions with key roles in the life cycle of the parasite. The predicted PPI networks can be visualized and downloaded at http://orthohpi.jensenlab.org .

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Section: Discussionmentioning

confidence: 99%

Analysis of Predicted Host–Parasite Interactomes Reveals Commonalities and Specificities Related to Parasitic Lifestyle and Tissues Tropism

et al. 2019

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“…These proteins are capable of modulating kainate receptors and their effect depend greatly upon the receptor composition [120,121]. Both proteins increase current amplitude, and glutamate affinity for all homomeric receptors, and significantly reduce inward rectification, but their modulation on desensitization kinetics is different [86,120,121]. kinetics.…”

Section: The Kainate Receptor Auxiliary Subunitsmentioning

confidence: 98%

“…However, a study carried out in NETO1 Knockout mice demonstrated that NMDA mediated currents were not affected by this mutation [86]. On the other hand, Amyloid Precursor Protein (APP) infusion increases NMDAR evoked currents [87] and together with its related proteins, amyloid precursor-like proteins 1 and 2 (APLP1 & APLP2), enhance surface NMDA receptors expression in vitro [88].…”

Section: The Nmda Receptor Auxiliary Subunitsmentioning

confidence: 98%

Advances in the pharmacology of lGICs auxiliary subunits

Galaz

Barra

Figueroa

et al. 2015

Pharmacological Research

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“…Prior proteomic studies have described alterations in amounts of eosinophil proteins in subjects with atopic dermatitis and eosinophilia ( 46 ), mildly elevated eosinophil counts associated with seasonal birch pollen allergy ( 47 ), and eosinophilia associated with Fasciola hepatica infection ( 48 ). In these studies, eosinophil proteins from affected individuals or healthy controls were separated by two-dimensional electrophoresis to produce high-resolution maps of protein-stained spots.…”

Section: Prospects For the Futurementioning

confidence: 99%

Proteomics of Eosinophil Activation

et al. 2017

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We recently identified and quantified >7,000 proteins in non-activated human peripheral blood eosinophils using liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS) and described phosphoproteomic changes that accompany acute activation of eosinophils by interleukin-5 (IL5) (1). These data comprise a treasure trove of information about eosinophils. We illustrate the power of label-free LC–MS/MS quantification by considering four examples: complexity of eosinophil STATs, contribution of immunoproteasome subunits to eosinophil proteasomes, complement of integrin subunits, and contribution of platelet proteins originating from platelet–eosinophil complexes to the overall proteome. We describe how isobaric labeling enables robust sample-to-sample comparisons and relate the 220 phosphosites that changed significantly upon treatment with IL5 to previous studies of eosinophil activation. Finally, we review previous attempts to leverage the power of mass spectrometry to discern differences between eosinophils of healthy subjects and those with eosinophil-associated conditions and point out features of label-free quantification and isobaric labeling that are important in planning future mass spectrometric studies.

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Altered Eosinophil Proteome in a Patient with Hypereosinophilia from Acute Fascioliasis

Abstract: We used comparative proteomics to analyze eosinophils from a patient with hypereosinophilia due to fascioliasis. Using 2-dimensional electrophoresis and mass spectrometry, we demonstrated that the eosinophil proteome was significantly altered compared to those of healthy controls.

Cited by 8 publications

References 13 publications

Analysis of Predicted Host–Parasite Interactomes Reveals Commonalities and Specificities Related to Parasitic Lifestyle and Tissues Tropism

Analysis of Predicted Host–Parasite Interactomes Reveals Commonalities and Specificities Related to Parasitic Lifestyle and Tissues Tropism

Advances in the pharmacology of lGICs auxiliary subunits

Proteomics of Eosinophil Activation

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