Altered expression of extracellular matrix and proteinases in noble rat prostate gland after long‐term treatment with sex steroids

Li, S.C.; Chen, G.F.; Chan, Peter S.; Choi, H.L.; Ho, Shuk‐Mei; Chan, F.H.Y.

doi:10.1002/pros.1118

Cited by 27 publications

(25 citation statements)

References 58 publications

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“…Sex steroid regulation of MMP expression/activity has been previously suggested because the levels of breast tumor MMP-9 and TIMP-1 vary with phases of the menstrual cycle (31). Conversely, rat prostate glands showed increased MMP activity after long-term exposure to androgen and estrogen (19).…”

Section: Discussionmentioning

confidence: 99%

Inhibition of tumor necrosis factor receptor-1-mediated pathways has beneficial effects in a murine model of postischemic remodeling

Ramani

Mathier

Wang

et al. 2004

American Journal of Physiology-Heart and Circulatory Physiology

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man. Inhibition of tumor necrosis factor receptor-1-mediated pathways has beneficial effects in a murine model of postischemic remodeling. Am J Physiol Heart Circ Physiol 287: H1369 -H1377, 2004; 10.1152/ajpheart.00641.2003.-The aim of the present study was to investigate the importance of tumor necrosis factor (TNF)-␣ receptor-1 (TNFR1)-mediated pathways in a murine model of myocardial infarction and remodeling. One hundred and ninety-four wild-type (WT) and TNFR1 gene-deleted (TNFR1KO) mice underwent left coronary artery ligation to induce myocardial infarction. On days 1, 3, 7, and 42, mice underwent transesophageal echocardiography. Hearts were weighed, and the left ventricle (LV) was assayed for matrix metalloproteinase (MMP)-2 and -9 activity and for tissue inhibitor of MMP (TIMP)-1 and -2 expression. Deletion of the TNFR1 gene substantially improved survival because no deaths were observed in TNFR1KO mice versus 56.4% and 18.2% in WT males and females, respectively (P Ͻ 0.002). At 42 days, LV remodeling, assessed by LV function (fractional area change of 31.9 Ϯ 7.9%, 32.2 Ϯ 7.7%, and 21.6 Ϯ 7.1% in TNFR1KO males, TNFR1KO females, and WT females, respectively, P Ͻ 0.04), and hypertrophy (heart weight-tobody weight ratios of 5.435 Ϯ 0.986, 5.485 Ϯ 0.677, and 6.726 Ϯ 0.704 mg/g, P Ͻ 0.04) were ameliorated in TNFR1KO mice. MMP-9 activity was highest at 3 days postinfarction and was highest in WT males (1.9 Ϯ 0.4 4, 3.6 Ϯ 0.24, 1.15 Ϯ 0.28, and 1.3 Ϯ 1.2 ng/100 g protein, respectively, in TNFR1KO males, WT males, TNFR1KO females, and WT females, respectively, P Ͻ 0.002), whereas at 3 days TIMP-1 mRNA fold upregulation compared with type-and sexmatched controls was lowest in WT males (138.32 Ϯ 13.05, 46.74 Ϯ 5.43, 186.09 Ϯ 28.07, and 101.76 Ϯ 22.48, respectively, P Ͻ 0.002). MMP-2 and TIMP-2 increased similarly in all infarcted groups. These findings suggest that the benefits of TNFR1 ablation might be attributable at least in part to the attenuation of cytokine-mediated imbalances in MMP-TIMP activity. extracellular matrix; left ventricular function; myocardial infarction ISCHEMIC HEART DISEASE is the major cause of congestive heart failure (CHF) in the United States. The adverse effects of left ventricular (LV) remodeling after myocardial infarction (MI) have been recognized as a primary factor in the development of ischemic cardiomyopathy and CHF (7,37,40). After the heart undergoes coronary occlusion, the infarct zone is characterized by cardiomyocyte cell death, degradation of the extracellular matrix (ECM), and neutrophil infiltration, followed by granulation tissue formation and maturation of granulation tissue into scar (6,25,30). However, remodeling also occurs in areas distant to the infarct zone and includes hypertrophy and dilatation. Recent improvements in mortality and morbidity after MI can be attributed to treatment modalities directed against late post infarction remodeling [i.e., dilation of the surviving myocardium (24)]. However, adverse outcomes, both early and late, are still common in t...

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Section: Discussionmentioning

confidence: 99%

Inhibition of tumor necrosis factor receptor-1-mediated pathways has beneficial effects in a murine model of postischemic remodeling

Ramani

Mathier

Wang

et al. 2004

American Journal of Physiology-Heart and Circulatory Physiology

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“…Notably, many of these physiologic processes become active during puberty or are augmented during the pubertal growth spurt. Moreover, pubertal hormones, specifically growth hormone and IGFs (36)(37)(38)(39), androgens (40) and estrogen (41,42), have all been shown to regulate MMP expression and/or secretion.…”

Section: Discussionmentioning

confidence: 99%

Physiological matrix metalloproteinase concentrations in serum during childhood and adolescence, using Luminex® Multiplex technology

Thrailkill¹,

Moreau²,

Cockrell³

et al. 2005

Clinical Chemistry and Laboratory Medicine (CCLM)

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Matrix metalloproteinases are a family of zinc-dependent proteinases which are involved in the breakdown and remodeling of extracellular matrix. As children grow and adolescents reach pubescence, their bodies undergo changes that require age-related morphogenesis of the extracellular matrix, possibly requiring unique patterns of matrix metalloproteinase (MMP) expression during periods of rapid tissue growth (i.e., childhood) or accelerated tissue remodeling and expansion (i.e., adolescence). Therefore, we have characterized age-specific and gender-specific differences in circulating concentrations of MMPs (specifically MMP-1, -2, -3, -8 and -9) in 189 serum samples obtained from healthy subjects, aged 2−18 years. MMP concentrations were measured using Fluorokine® MultiAnalyte Profiling kits and a Luminex® Bioanalyzer, as well as by commercial ELISA. Serum levels of MMP-1, -2, -3, -8, and -9 in healthy pediatric subjects represent log-normal distributions. MMP-2 was significantly negatively correlated with age (r=−0.29; p<0.001), while MMP-3 was significantly positively correlated with age (r=0.38; p<0.001). Although plasma, not serum, is considered the appropriate blood sample for measurement of MMP-8 and -9, serum levels of MMP-8 and -9 were also found to be highly positively correlated with each other (r=0.76; p<0.01). MMP results obtained by Fluorokine® MultiAnalyte Profiling methods correlated well with conventional ELISA methods and use of this technology provided several advantages over ELISA.

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“…It is well-known that cell proliferation in the normal prostate and in prostate cancer is mainly controlled by testosterone following intracellular conversion to DHT (Ross & Henderson 1994, ImperatoMcGinley et al 1992. However, it has also been demonstrated that the remodeling of prostate stromal tissue via testosterone may play a role in the early stage of prostate carcinogenesis, which is generally considered to be favorable for tumor development (Li et al 2001). Therefore, 17b-HSD5 may be involved in increasing the local concentration of testosterone in prostate cancer tissues, resulting in the progression, invasion, and further development of prostate cancer.…”

Section: Correlation Between Immunoreactivities and Clinicopathologicmentioning

confidence: 99%

In situ androgen producing enzymes in human prostate cancer

Nakamura

Suzuki

Nakabayashi

et al. 2005

Endocr Relat Cancer

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Androgens have been proposed to be actively produced in situ in human prostate cancer. These locally produced androgens have also been considered to play important roles in the pathogenesis and development of prostate cancer. Therefore, it is important to examine the status of this in situ androgen metabolism and/or synthesis in detail in order to improve the clinical response to hormonal therapy in patients diagnosed with prostate cancer. Several studies have previously demonstrated the expression of androgen-producing enzymes such as 5a-reductase types 1 and 2, and 17b-hydroxysteroid dehydrogenase type 5 (17b-HSD5), in human prostate carcinoma cells. However, their biological significance has remained largely unknown. In this study, we evaluated the immunoreactivities of these steroidogenic enzymes in human prostate cancer obtained from surgery (n=70), and correlated the findings with clinicopathological features of the patients. 17b-HSD5 immunoreactivity was detected in 54 cases (77%), 5a-reductase type 1 in 51 cases (73%) and 5a-reductase type 2 in 39 cases (56%). 5a-reductase type 2 immunoreactivity was significantly correlated with that of androgen receptor (AR), and 17b-HSD5 positive cases were significantly associated with clinical stage (TNM stage pT3 vs pT2). These data all suggest that androgenproducing enzymes, such as 5a-reductase type 1 and type 2, and 17b-HSD5 are expressed in a majority of prostate cancers, and are involved in the local production and actions of androgens in prostate cancers.

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Altered expression of extracellular matrix and proteinases in noble rat prostate gland after long‐term treatment with sex steroids

Cited by 27 publications

References 58 publications

Inhibition of tumor necrosis factor receptor-1-mediated pathways has beneficial effects in a murine model of postischemic remodeling

Inhibition of tumor necrosis factor receptor-1-mediated pathways has beneficial effects in a murine model of postischemic remodeling

Physiological matrix metalloproteinase concentrations in serum during childhood and adolescence, using Luminex® Multiplex technology

In situ androgen producing enzymes in human prostate cancer

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