Altered expression of microRNAs in the response to ER stress

Dai, Limin; Huang, Chuan; Chen, Liang; Ge, Shengfang; Li, Zhaoyong

doi:10.1007/s11434-014-0657-z

Cited by 9 publications

(7 citation statements)

References 26 publications

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“…miR-3648 is a human specific miRNA, and no ortholog exists in great apes and the other mammals [ 32 ]. Sequencing data previously generated in our lab showed miR-3648 to be upregulated in ER stress [ 26 ]. We checked the endogenous expression of miR-3648 in different human cell lines and tissues, and found this miRNA to be expressed in all the tested cell lines and tissues ( Figure 1 A,B).…”

Section: Resultsmentioning

confidence: 99%

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Induction of miR-3648 Upon ER Stress and Its Regulatory Role in Cell Proliferation

Farooq

Awan

Shah

et al. 2017

IJMS

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MicroRNAs (miRNAs) play important roles under multiple cellular conditions including endoplasmic reticulum (ER) stress. We found that miR-3648, a human specific microRNA, was induced under ER stress. Moreover, Adenomatous polyposis coli 2 (APC2), a tumor suppressor and a negative regulator of Wnt signaling, was found to be the direct target of miR-3648. Levels of APC2 were downregulated when cells were under ER stress or after overexpressing miR-3648. Inhibition of miR-3648 by antagomir increased APC2 levels and decreased cell proliferation. Conversely, when miR-3648 was overexpressed, APC2 levels were decreased and the cell growth increased. Our data demonstrated that ER stress mediated induction of miR-3648 in human cells, which then downregulated APC2 to increase cell proliferation.

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Section: Resultsmentioning

confidence: 99%

“…ER stress alters an array of cell signaling cascades including the Wnt/β-catenin pathway [ 24 , 25 ]. Multiple miRNAs have also been shown to participate in cellular responses to ER stress [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Induction of miR-3648 Upon ER Stress and Its Regulatory Role in Cell Proliferation

Farooq

Awan

Shah

et al. 2017

IJMS

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“…This variant might also increase the affinity of hsa-miR-3609 (energy charge in wild-type allele: 15.40 Kcal vs. mutant allele: 16.30 Kcal) and hsamiR-548ah-5p (energy charge in wild-type allele: 13.60 Kcal vs. mutant allele: 14.60 Kcal) ( Table 1). Some studies reported overexpression of hsa-miR-3609 under endoplasmic reticulum stress (32) and in lung cancer (33). Considering the predicted target mRNAs of hsa-miR-548, functional enrichment analysis showed that the …”

Section: Discussionmentioning

confidence: 99%

Prioritization of rs187728237 and rs80320514 as miRNA-related Variants of Human AEG-1 Gene

et al. 2018

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Background and Objectives: 3' untranslated region (3'UTR) single nucleotide polymorphisms (SNPs) represent genetic variations that may potentially affect binding of miRNA to coding genes, potentially leading to complex disorders. We aimed to perform in silico analysis of the potential phenotypic effect of 3'UTR SNPs on human astrocyte elevated gene-1 (AEG-1), a newly identified candidate cancer gene.Methods: We gathered a list of all 3'UTR SNPs located in the human AEG-1 gene from the SNP database. Analysis of the potential effects was done using MirSNP and MicroSNiper.Results: Analysis by the MirSNP estimated that rs187728237 might increase the affinity of two miRNAs and decrease the affinity of 10 other miRNAs to the AEG-1 transcript. Moreover, MicroSNiPer showed that rs80320514 might affect 24 putative miRNA binding sites in the 3'UTR of AEG-1.Conclusion: Based on our findings, it can be concluded that the 3'UTR SNPs located in the human AEG-1 gene may be within the miRNA targets of the transcript, therefore affecting the stability of putative miRNA-target interactions.

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“…miRNAs are known to play pivotal roles in myriad of cellular functions ranging from development to disease and response to endogenous or exogenous stimuli. [31][32][33][34][35] To assess the involvement of a miRNA in a biological process, computational methods and molecular biology approaches are routinely used. Computational methods generally rely on phylogenetically conserved miRNA binding sites in the target gene and free energy of binding.…”

Section: Discussionmentioning

confidence: 99%

Comparing two approaches of miR-34a target identification, biotinylated-miRNA pulldown vs miRNA overexpression

et al. 2017

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microRNAs (miRNAs) are critical regulators of gene expression. For elucidating functional roles of miRNAs, it is critical to identify their direct targets. There are debates about whether pulldown of biotinylated miRNA mimics can be used to identify miRNA targets or not. Here we show that biotin-labelled miR-34a can be loaded to AGO2, and AGO2 immunoprecipitation can pulldown biotinylated miR-34a (Bio-miR pulldown). RNA-sequencing (RNA-seq) of the Bio-miR pulldown RNAs efficiently identified miR-34a mRNA targets, which could be verified with luciferase assays. In contrast to the approach of Bio-miR pulldown, RNA-seq of miR-34a overexpression samples had limited value in identifying direct targets of miR-34a. It seems that pulldown of 3'-Biotin-tagged miRNA can identify bona fide microRNA targets at least for miR-34a.

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Altered expression of microRNAs in the response to ER stress

Cited by 9 publications

References 26 publications

Induction of miR-3648 Upon ER Stress and Its Regulatory Role in Cell Proliferation

Induction of miR-3648 Upon ER Stress and Its Regulatory Role in Cell Proliferation

Prioritization of rs187728237 and rs80320514 as miRNA-related Variants of Human AEG-1 Gene

Comparing two approaches of miR-34a target identification, biotinylated-miRNA pulldown vs miRNA overexpression

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