Altered findings of hepatic arteriography after radiofrequency ablation of hepatocellular carcinoma: comparison of pre-ablation and post-ablation angiograms

Kim, Byung Moon; Cho, Jae Hyun; Won, Je Hwan; Lee, Do Yun; Lee, Jong Tae; Kim, Hyun Cheol; Park, Sung Il

doi:10.1007/s00261-006-9059-2

Cited by 1 publication

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“…One fairly successful application of focused hyperthermia is percutaneous tumor ablation (13)(14)(15), which utilizes radiofrequency (RF) current to heat tumor tissue to cytotoxic temperatures (nearing 1008C) by means of a needle electrode. This approach leads to protein denaturation and loss of cytosolic and mitochondrial enzyme activity that results in adequate control of tumors less than 3 cm in diameter in patients for whom surgical resection is not a viable option (16,17).…”

Section: Introductionmentioning

confidence: 99%

Time and Dose Dependence of Pluronic Bioactivity in Hyperthermia-Induced Tumor Cell Death

Krupka

Dremann

Exner

2009

Exp Biol Med (Maywood)

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Pluronic block copolymers have been shown to sensitize cancer cells resulting in an increased activity of antineoplastic agents. In the current study we examined a new application of Pluronic bioactivity in potentiating hyperthermia-induced cancer cell injury. DHD/K12/TRb rat adenocarcinoma cells were exposed to low-grade hyperthermia at 43°C with or without Pluronic P85 or Pluronic L61. A range of Pluronic doses, pre-exposure and heat exposure durations were investigated, and the test conditions were optimized. Treatment efficacy was assessed by measurement of intracellular ATP and mitochondrial dehydrogenase activity. Both P85 and L61 in synergy with heat reduced cell viability appreciably compared to either heat or Pluronic alone. Under optimal conditions, P85 (10 mg/ml, 240 mins) combined with 15 mins heat reduced intracellular ATP to 60.1 ± 3.5% of control, while heat alone and P85 without heat caused a negligible decrease in ATP of 1.2% and 3.8%, respectively. Similarly, cells receiving 120 mins pre-exposure of L61 (0.3 mg/ml) showed reduction in intracellular ATP to 14.1 ± 2.1% of control. Again, heat or L61 pre-exposure alone caused a minor decrease in levels of intracellular ATP (1.5% and 4.4%, respectively). Comparable results were observed when viability was assessed by mitochondrial enzyme activity. Survival studies confirmed that the loss of viability translates to a long-term reduction in proliferative activity, particularly for L61 treated cells. Based on these results, we conclude that Pluronic is effective in improving hyperthermic cancer treatment in vitro by potentiating heat-induced cytotoxicity in a concentration and time dependent manner.

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