Altered glycosylation and expression of plasma alpha-1-acid glycoprotein and haptoglobin in rheumatoid arthritis

Autoimmune diseases (ADs) are currently treated with anti-inflammatory and immunosuppressive drugs, aimed at reducing symptoms of disease in order to improve quality of life for patients. However, for a significant number of patients these therapies are ineffective, leading to an increased risk of irreversible damage and eventual disability in certain cases. Growing evidence has implicated glycosylated proteins and their cognate receptors in modulation of the autoimmune response. This review will summarize these findings with particular focus on sialic acid-binding immunoglobulin-like lectin (Siglec)-1 and Siglec-2 involvement in AD. Fluctuations in these glycosylation-dependent pathways could act as sentinels of disease activity or drug responses. If validated, protein modification and cellular response markers could help clinicians achieve remission earlier.

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“…Saroha et al [21] reported a unique monosaccharide pattern of Haptoglobin and acid glycoprotein to be specifically linked with RA pathogenesis.…”

Section: Acute-phase Response Proteinsmentioning

confidence: 99%

Siglec‐1 and ‐2 as potential biomarkers in autoimmune disease

Eakin

Bustard

McGeough

et al. 2016

Proteomics Clinical Apps

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“…Two dimensional gel electrophoresis (2DE), staining, image analysis, matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) and database identification of proteins One hundred micrograms of protein from both AAL enriched and albumin-IgG depleted plasma was subjected to 2DE analysis as described by Saroha et al [8]. Minimum three 2D gels of each AAL enriched and albumin-IgG depleted plasma were run.…”

Section: Glycoprotein Enrichment Using Aleuria Aurantia Lectin (Aal)mentioning

confidence: 99%

“…Minimum three 2D gels of each AAL enriched and albumin-IgG depleted plasma were run. This was followed by mass spectrometry compatible silver staining for visualization of protein spots [8]. The gels were scanned as 16 bit gray scale image by gel documentation system by Alpha Innotech Corp., USA.…”

Section: Glycoprotein Enrichment Using Aleuria Aurantia Lectin (Aal)mentioning

confidence: 99%

“…The gels were scanned as 16 bit gray scale image by gel documentation system by Alpha Innotech Corp., USA. The spot density of each spot was manually calculated using Alpha Digidoc 1201 software and the differentially expressed spots were cut and digested with trypsin for MALDI-TOF analysis as described earlier [8].…”

Section: Glycoprotein Enrichment Using Aleuria Aurantia Lectin (Aal)mentioning

confidence: 99%

“…The Western blotting was performed using human anti-ficolin 3 (Santa Cruz) antibody as described earlier [8].…”

Section: Western Blottingmentioning

confidence: 99%

See 2 more Smart Citations

Enhanced expression and fucosylation of ficolin3 in plasma of RA patients

Roy¹,

Biswas²,

Saroha³

et al. 2013

Clinical Biochemistry

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High‐throughput immunoaffinity enrichment and N‐glycan analysis of human plasma haptoglobin

Šimunović

Gašperšič

Černigoj

et al. 2022

Biotech & Bioengineering

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Haptoglobin (Hp) is a positive acute phase protein, synthesized in the liver, with four N-glycosylation sites carrying mainly complex type N-glycans. Its glycosylation is altered in different types of diseases but still has not been extensively studied mainly due to analytical challenges, especially the lack of a fast, efficient, and robust high-throughput Hp isolation procedure. Here, we describe the development of a high-throughput method for Hp enrichment from human plasma, based on monolithic chromatographic support in immunoaffinity mode and downstream Hp N-glycome analysis by hydrophilic interaction ultrahigh-performance liquid chromatography with fluorescent detection (HILIC-UHPLC-FLR). Chromatographic monolithic supports in a 96-well format enable fast, efficient, and robust Hp enrichment directly from diluted plasma samples. The N-glycome analysis demonstrated that a degree of Hp deglycosylation differs depending on the conditions used for N-glycan release and on the specific glycosylation site, with Asn 241 being the most resistant to deglycosylation under tested conditions. HILIC-UHPLC-FLR analysis enables robust quantification of 28 individual chromatographic peaks, in which N-glycan compositions were determined by UHPLC coupled to electrospray ionization quadrupole time of flight mass spectrometry. The developed analytical approach enables fast evaluation of total Hp N-glycosylation and is applicable in large-scale studies.

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Altered glycosylation and expression of plasma alpha-1-acid glycoprotein and haptoglobin in rheumatoid arthritis

Cited by 36 publications

References 29 publications

Siglec‐1 and ‐2 as potential biomarkers in autoimmune disease

Siglec‐1 and ‐2 as potential biomarkers in autoimmune disease

Enhanced expression and fucosylation of ficolin3 in plasma of RA patients

High‐throughput immunoaffinity enrichment and N‐glycan analysis of human plasma haptoglobin

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