Altered Liver Morphology and Enzymes in Streptozotocin Induced Diabetic Rats

Abstract

ZAFAR, M.; NAEEM-UL-HASSAN NAQVI, S.; AHMED, M. & KAIMKHANI, Z. A. Altered liver morphology and enzymes in streptozotocin induced diabetic rats. Int. J. Morphol., 27(3):719-725, 2009. SUMMARY:This study was undertaken to evaluate the relationship and effects of diabetes on liver morphology, architecture and function. The hepatic effects of diabetes were evaluated in vivo using streptozotocin (STZ)-induced diabetic rats as an experimental model. The degree of hepatic dysfunction was measured by using biochemical parameters like serum transaminases (ALT and AST), alkaline phosphatase (ALP)and pseudocholinesterase (PChE) while the histopathological studies were carried out to support the enzymic Parameters. The aim of the study was to investigate the association between diabetic hepatic complications and liver enzyme alterations. This study was performed in the . Diabetes was induced by a single dose of STZ (45 mg/kg, b.w.) given intraperitoneally in sodium citrate buffer at pH 4.5. Eighty albino rats were divided into five groups: control (A) and STZ treated (B, C, D, and E) which were sacrificed 2, 4, 6 and 8 weeks post treatment respectively. Histopathological examination of liver showed accumulation of lipid droplets, lymphocytic infiltration, increased fibrous content, dilatation and congestion of portal vessels and proliferation of bile ducts. Increased levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), ALP and PChE were observed in the liver. It seems that the diabetic complications in the liver like hepatocyte destruction etc. are likely to be due to alterations in enzyme levels. ZAFAR, M.; NAEEM-UL-HASSAN NAQVI, S.; AHMED, M. & KAIMKHANI, Z. A. Altered liver morphology and enzymes in streptozotocin induced diabetic rats. Int. J. Morphol., 27(3):719-725, 2009.

Antidiabetic and anti-oxidant activities of the methanol leaf extract of <i>Vernonia amygdalina</i> in alloxan-induced diabetes in Wistar rats

“…This blood glucose lowering effect is the result of the bioactive secondary components of the plant. Relationships between hyperglycaemia and decrease in body weight of experimental animals have been reported (Igile et al 1995;Zafar et al 2009). In this study, there was a significant (p < 0.05) decrease in body weight of the diabetic untreated group compared with the normal control and extract-treated animals ( Table 2).…”

In Vivo Evaluation of Anti Diabetic, Hypolipidemic, Antioxidative Activities of Saudi Date Seed Extract on Streptozotocin Induced Diabetic Rats

“…Several workers have reported a significant decrease in body weight of STZ diabetic rats [43]. The reduction in the body weight of the diabetic animals could be explained on the basis of loss of structural proteins as these structural proteins contribute to body weight [44].…”

Anti-Lipidemic Effect of Combined Leaf Extracts of &lt;i&gt;Moringa oleifera&lt;/i&gt; and &lt;i&gt;Peristrophe bicalyculata&lt;/i&gt; in Alloxan-Induced Diabetic Rats

“…More so, a metabolic impairment leading to imbalance in energy can also result from impressed factors including drastic changes in environmental conditions, exposure to drugs, toxicants, pollutants and the likes [29]. Studies have shown an association between hyperglycemia and decrease in body weight of experimental animals [30]. Consequently, changes in weight are usually a fundamental index of physiological or pathological state of an experimental animal.…”