2020
DOI: 10.1016/j.molcel.2019.11.007
|View full text |Cite
|
Sign up to set email alerts
|

Altered m6A Modification of Specific Cellular Transcripts Affects Flaviviridae Infection

Abstract: Highlights d Flaviviridae infection alters m 6 A modification of specific cellular mRNAs d Innate immune and ER stress signaling contribute to altered m 6 A modification d Gain of m 6 A regulates RIOK3 translation, and loss of m 6 A influences CIRBP splicing d m 6 A-altered mRNAs encode factors that affect Flaviviridae infection

Help me understand this report
View preprint versions

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

6
151
1

Year Published

2020
2020
2023
2023

Publication Types

Select...
5
2
1

Relationship

3
5

Authors

Journals

citations
Cited by 147 publications
(158 citation statements)
references
References 124 publications
6
151
1
Order By: Relevance
“…Infectious stocks of a cell culture-adapted strain of DENV2-NGC and ZIKV-DAKAR were generated and titered, as described (51). ZIKV-DAKAR (Zika virus/A,africanus-tc/SEN/1984/41525-DAK) was provided by Dr. Scott Weaver at University of Texas Medical Branch.…”
Section: Methodsmentioning
confidence: 99%
“…Infectious stocks of a cell culture-adapted strain of DENV2-NGC and ZIKV-DAKAR were generated and titered, as described (51). ZIKV-DAKAR (Zika virus/A,africanus-tc/SEN/1984/41525-DAK) was provided by Dr. Scott Weaver at University of Texas Medical Branch.…”
Section: Methodsmentioning
confidence: 99%
“…Another method often used for MeRIP-seq peak detection is MACS2, which was originally designed to detect protein binding sites in DNA from chromatin immunoprecipitation sequencing (ChIP-seq). We compared m 6 A (m) peak detection by exome-Peak, MeTPeak, MeTDiff, and MACS2 31,[47][48][49] in seven replicates of MeRIP-seq data obtained from mouse cortices under basal conditions 34 , and in 12 replicates of MeRIP-seq data we generated from human liver Huh7 cells 50 . The intersect between all tools tested was high, and we saw minimal differences in DRAC motif enrichment, which we use to provide an estimate of tool precision in the absence of true positive m 6 A sites ( Supplementary Fig.…”
Section: Detection Of Peaks Across Replicates Experiments and Cell mentioning
confidence: 99%
“…We next assessed the correlation between m 6 A enrichment observed using MeRIP-seq and MeRIP-RT-qPCR using data from our recent work that identified 58 peak changes in m 6 A in Huh7 cells following infection by four different viruses 50 . For those experiments, we again selected peaks that change based on results from the union of QNB and the GLM approaches.…”
Section: Merip-rt-qpcr Validationmentioning
confidence: 99%
“…In this system, we generated a construct with an exogenous plasmid-based promoter to drive expression of an RNA containing the splice donor exon of the viral late tripartite leader, as much intervening Fiber intron as possible without including additional viral genes or splice sites, and the 5' region of Fiber that encompasses the meRIP-seq peak. This viral cassette was fused to a Renilla luciferase gene in which all m 6 A DRACH motifs were silently mutated 34 . These constructs allow transgene expression with wildtype viral context (WT Fiber), or with all DRACH sites present in both exonic and intronic regions silently mutated to ablate deposition of METTL3-dependent m 6 A (m A Mut Fiber, mutations shown below in Figure 6h).…”
Section: Splicing Efficiency Of Late Viral Rna Is Mediated By M 6 Amentioning
confidence: 99%
“…Fiber-Transgene constructs were created from the previously generated m 6 A-null psiCheck2 reporter plasmid 34 Table 1. DNA transfections were performed using the standard protocol for Lipofectamine2000 (Invitrogen).…”
Section: Plasmids Sirna and Transfectionsmentioning
confidence: 99%