Altered Metabolic Phenotype of Immune Cells in a Spontaneous Autoimmune Uveitis Model

Barfüßer, Claudia; Wiedemann, Carmen; Hoffmann, Anne Lisbeth; Hirmer, Sieglinde; Deeg, Cornelia A.

doi:10.3389/fimmu.2021.601619

Cited by 4 publications

(2 citation statements)

References 55 publications

(84 reference statements)

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“…Altogether, many of the immunological processes described for human autoimmune diseases [155,156] have also been found in horses suffering from ERU [133,134,142,[157][158][159][160][161][162][163][164][165][166][167][168][169][170][171][172]. Only a few authors considered that, in addition to autoimmune etiology, the permanent presence of leptospires in an "atypical" form could also be considered as a cause of recurrent episodes of uveitis [63,173,174].…”

Section: Historical and Preliminary Remarks On The Etiology Of Erumentioning

confidence: 99%

Infectious Uveitis in Horses and New Insights in Its Leptospiral Biofilm-Related Pathogenesis

2022

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Uveitis is a sight-threatening eye disease in equids known worldwide that leads to considerable pain and suffering. By far the most common type of uveitis in Germany and neighboring countries is classical equine recurrent uveitis (ERU), which is caused by chronic intraocular leptospiral infection and is the main cause of infectious uveitis in horses. Other infectious causes are extremely rare and are usually clinically distinguishable from ERU. ERU can be treated very effectively by vitreous cavity lavage (vitrectomy). For proper indications of this demanding surgery, it is necessary to differentiate ERU from other types of uveitis in which vitrectomy is not helpful. This can be conducted on the basis of anamnesis in combination with ophthalmologic findings and by aqueous humor examination. During vitrectomy, vitreous material is obtained. These vitreous samples have historically been used for numerous etiologic studies. In this way, a chronic intraocular leptospiral infection has been shown to be the cause of typical ERU and, among other findings, ERU has also been recognized as a biofilm infection, providing new insights into the pathogenesis of ERU and explaining some thus far unexplainable phenomena of ERU. ERU may not only have transmissible aspects to some types of uveitis in humans, but may also serve as a model for a spontaneously occurring biofilm infection. Vitreous material obtained during therapeutically indicated vitrectomy can be used for further studies on in vivo biofilm formation, biofilm composition and possible therapeutic approaches.

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Section: Historical and Preliminary Remarks On The Etiology Of Erumentioning

confidence: 99%

Infectious Uveitis in Horses and New Insights in Its Leptospiral Biofilm-Related Pathogenesis

2022

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“…Due to the complex aetiology and pathogenesis, the treatment of uveitis is difficult (Barfüßer et al, 2021). Glucocorticoids, immunosuppressants and other drugs are often used in the clinical treatment of autoimmune uveitis, but long-term use may lead to many adverse reactions (Cunningham & Wender, 2010;Okada, 2005;Díaz-Llopis et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Effects of Qingli Gandan decoction on IL-23/IL-17 in rats with experimental autoimmune uveitis

et al. 2022

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In this study, through animal experiments, we investigated the effects of Qingli Gandan decoction on the IL-23/IL-17 signalling pathway and the expression of CD4+/IL-17A+ cells in EAU rats. We then predicted the potential mechanisms of the action. First, Lewis rats were divided using the random number table method into a (1) control group, (2) model group and (3) TCM group. The TCM group was given a Qingli Gandan prescription by gavage after an intervention model. Next, blood, aqueous humour and vitreous were collected from each group on days 4, 8, 12, 16 and 20 after immunisation. An enzyme linked immunosorbent assay (ELISA) was used to detect the protein concentrations of IL-17 and IL-23 in a serum, aqueous humour and vitreous of rats on different immunisation days, and the percentage of CD4+/IL-17A+ cells in rat spleen was detected by flow cytometry. The peak concentration of serum IL-17 protein in group 3 was statistically lower than that in group 2 (P < 0.05) on days 4, 8 and 12 of immunisation. However, the peak concentration of serum IL-23 protein in group 3 was statistically lower than in group 2 (P < 0.05) on days 8 and 12 of immunisation; in addition, on days 8 and 12, the protein concentrations of IL-23 and IL-17 in the aqueous humour group 3 were all lower than those in group 2. On days 4, 8 and 12, the protein concentrations of IL-23 and IL-17 in vitreous in group 3 were significantly lower than those in group 2. We found that the proportion of CD4+/ IL-17A+ cells in the groups 2 and 3 increased on day 8 compared with group 1 (P < 0.05). The proportion of CD4+/IL-17A+ cells decreased in group 3 compared with group 2 (P < 0.05).At last, we think Qingli Gandan can reduce the onset of EAU in rats and treat it, presumably because of inhibiting the IL-23/IL-17 axis. It may inhibit the expression of CD4+/IL-17A+ cells and play a role in inhibiting the IL-23/IL-17 signalling pathway.

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Causal role of immune cells in uveitis: a Mendelian randomization study

Pu,

Zhao,

Duan

et al. 2024

Front. Med.

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BackgroundUveitis refers to a group inflammation affecting the uvea, retina, retinal blood vessels as well as vitreous body, which is one of the common causes of blindness. There is growing evidence linking different types of immune cells to uveitis, although it remains uncertain if these associations imply causal relationships. Recent advancements in high-density genetic markers like SNPs or CNVs for genotyping, along with the progress in genome-wide association studies (GWAS) technologies, have improved our understanding of the immunological mechanisms involved in ocular diseases. Therefore, our objective was to investigate the potential causal link between immune cells and uveitis using a Mendelian randomization study.MethodsThe exposure and outcome GWAS data for this study were sourced from an open-access database (https://gwas.mrcieu.ac.uk/). Two-sample MR analysis was utilized to evaluate the causal relationship between 731 immune cell features and uveitis. Various MR methods were employed to reduce bias and obtain dependable estimates of the causal link between the immune cell variables and the outcomes. Instrumental variable selection criteria were carefully chosen to enhance the accuracy and efficacy of the causal relationship between different immune cell types and the risk of uveitis.ResultsUsing two-sample MR, IVW modeling showed that GAD had significant effect on immunophenotypes. CD3 levels on CD45RA− CD4+ T cells (OR = 1.087, 95%CI = 1.029 ~ 1.147, p = 0.003) and CD3 levels on CM CD4+ T cells (OR = 1.086, 95%CI = 1.033 ~ 1.141, p = 0.001) were found to be elevated in cases of uveitis. HLA DR levels in CD14− CD16+ monocyte cells (OR = 0.735, 95% CI = 0.635 ~ 0.850, p < 0.001) and HLA DR levels in NK cells (OR = 0.910, 95% CI = 0.851 ~ 0.972, p = 0.005) were observed to be reduced in individuals with uveitis. Furthermore, Two cells were identified to be significantly associated with uveitis risk: HLA DR on in NK cells (OR = 0.938, 95%CI = 0.899 ~ 0.979, p = 0.003), HLA DR on CD14− CD16+ monocytes (OR = 0.924, 95%CI = 0.878 ~ 0.972, p = 0.002).ConclusionThis study highlights the intricate relationship between immune cells and generalized anxiety disorder using genetic methods, offering valuable insights for future clinical investigations.

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Altered Metabolic Phenotype of Immune Cells in a Spontaneous Autoimmune Uveitis Model

Cited by 4 publications

References 55 publications

Infectious Uveitis in Horses and New Insights in Its Leptospiral Biofilm-Related Pathogenesis

Infectious Uveitis in Horses and New Insights in Its Leptospiral Biofilm-Related Pathogenesis

Effects of Qingli Gandan decoction on IL-23/IL-17 in rats with experimental autoimmune uveitis

Causal role of immune cells in uveitis: a Mendelian randomization study

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