Altered microRNA expression profile with miR‐27b down‐regulation correlated with disease activity of oral lichen planus

Abstract

These data indicate that miRNAs may be the novel candidate biomarkers for the implication of miRNAs in the pathogenesis of OLP.

Genomewide miRNA profiling of oral lichenoid disorders and oral squamous cell carcinoma

“…Six of the 20 microRNAs in our OLD signature had been previously linked to OLD (Arão et al , ; Zhang et al , ; Gassling et al , ; Zuo et al , ), thus somehow validating the accuracy of our results. However, cross‐comparison of the deregulated microRNAs signatures described in the genomewide microRNA studies published so far along with ours identified just one microRNA that was consistently deregulated across the three studies, that is, miR‐146a, being these findings in line with previous microRNA‐candidate studies (Arão et al , ; Zuo et al , ).…”

“…It has been suggested that microRNAs may be used to predict the malignant transformation of oral premalignant lesions (Cervigne et al , ) and altered microRNA expression patterns have been associated with a number of physiological and pathological processes, including cancer, chronic inflammatory diseases, and other inflammatory conditions (Wu et al , ; De Sarkar et al , ). While much remains to be deciphered about the role of microRNAs in oral lichenoid disorders, recent studies have shown an altered microRNA expression profile that differs from that of the control group (Zhang et al , ; Gassling et al , ). In this respect, after interrogating the oral mucosa of seven patients with oral lichen planus and seven controls, Gassling et al () identified 16 deregulated microRNAs, of which several were functionally related to premalignant as well as to inflammatory events.…”

“…In this respect, after interrogating the oral mucosa of seven patients with oral lichen planus and seven controls, Gassling et al () identified 16 deregulated microRNAs, of which several were functionally related to premalignant as well as to inflammatory events. On the other hand, Zhang et al () studied three OLP samples and three control tissues and found a total of 46 differentially expressed microRNAs. It is important to note that in both studies, (i) the P ‐values used to select the deregulated microRNAs were not adjusted for multiple comparisons, and raw P ‐values were used probably due to the relatively low statistical power of these two studies; (ii) the number of samples analyzed is relatively small; and (iii) the selected markers were not validated on an independent cohort of patients.…”

“…Gassling et al () identified 24 differentially regulated microRNAs and 2694 transcripts after interrogating the mRNA/microRNA profiles from the oral mucosa of seven patients with OLP and seven healthy individuals. Zhang et al (Zhang et al , ) also compared the global pattern of microRNA expression in oral biopsies taken from three patients with oral lichen planus and found differential expression in 46 of the 667 microRNAs studied. However, the small sample size employed in these studies could have introduced a potential confounding factor.…”

Comparative studies of two methods for miRNA isolation from milk whey

“…This finding could also explain the low PCR efficiency of miRNA. Currently, NanoDrop and the Bioanalyzer are widely used to analyze the quality of RNA samples (Fleige and Pfaffl, 2006;Zhang et al, 2012). Among these, the Bioanalyzer 2100 instrument with an RNA 6000 Pico Kit (based on electropherograms) can also be used to analyze the distribution of RNA size in each RNA sample.…”