Altered nanofeature size dictates stem cell differentiation

Zouani, Omar F.; Chanseau, Christel; Brouillaud, Brigitte; Bareille, Reine; Deliane, Florent; Foulc, Marie-Pierre; Mehdi, Ahmad; Durrieu, Marie‐Christine

doi:10.1242/jcs.093229

Cited by 78 publications

(80 citation statements)

References 38 publications

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“…The authors provided further evidence that differentiation of skeletal stem cells cultured on nanotopographical surfaces, provided an equal and effective technique of differentiation of stem cells compared to chemical induction [33] . Zouani et al [34] similarly showed that hMSCs cultured on polyethylene terephthalate substrates with nano-depths of varying degrees, had higher osteogenic differentiation on the 100 nm patterns compared to the 10 nm patterns. The organization of the hMSCs on the 100 nm was believed to induce FA contacts, generating stress in the actin filaments.…”

Section: Surface Topographical Cluesmentioning

confidence: 99%

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Control of stem cell fate by engineering their micro and nanoenvironment

Griffin

Butler

Seifalian

et al. 2015

WJSC

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Stem cells are capable of long-term self-renewal and differentiation into specialised cell types, making them an ideal candidate for a cell source for regenerative medicine. The control of stem cell fate has become a major area of interest in the field of regenerative medicine and therapeutic intervention. Conventional methods of chemically inducing stem cells into specific lineages is being challenged by the advances in biomaterial technology, with evidence highlighting that material properties are capable of driving stem cell fate. Materials are being designed to mimic the clues stem cells receive in their in vivo stem cell niche including topographical and chemical instructions. Nanotopographical clues that mimic the extracellular matrix (ECM) in vivo have shown to regulate stem cell differentiation. The delivery of ECM components on biomaterials in the form of short peptides sequences has also proved successful in directing stem cell lineage. Growth factors responsible for controlling stem cell fate in vivo have also been delivered via biomaterials to provide clues to determine stem cell differentiation. An alternative approach to guide stem cells fate is to provide genetic clues including delivering DNA plasmids and small interfering RNAs via scaffolds. This review, aims to provide an overview of the topographical, chemical and molecular clues that biomaterials can provide to guide stem cell fate. The promising features and challenges of such approaches will be highlighted, to provide directions for future advancements in this exciting area of stem cell translation for regenerative medicine.

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Section: Surface Topographical Cluesmentioning

confidence: 99%

“…The organization of the hMSCs on the 100 nm was believed to induce FA contacts, generating stress in the actin filaments. Actin tension was found to be important in the osteogenic differentiation of the cells as the osteoblastic phenotype was inhibited by cytochalasin D [34] . …”

Section: Surface Topographical Cluesmentioning

confidence: 99%

Control of stem cell fate by engineering their micro and nanoenvironment

Griffin

Butler

Seifalian

et al. 2015

WJSC

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“…When ESCs are aggregated into embryoid bodies (EB), which mimic early stages of embryonic development, it becomes clear that geometrical signals also affect cell-cell interaction and hence guide stem cell differentiation [66]. The investigation of hexagonal patterns, random nanopits, disordered and ordered squares utilising on hMSCs electron beam lithography (EBL) showed that highly asymmetric structures resulted in highest osteogenic expression in the absence of growth factor supplemented medium [237] indicating that nano-sized topographical induction of stem cell differentiation is as effective as chemical induction [238]. Micropatterns have also been shown to affect cell shape.…”

Section: Topographical Signals As Regulators Of Stem Cell Fatementioning

confidence: 99%

Pluripotent Stem Cells and Their Dynamic Niche

Reinwald¹,

Bratt²,

Haj³

2016

Pluripotent Stem Cells - From the Bench to the Clinic

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Cell-seeded implants are a regenerative medicine strategy that aims to replace injured tissue and restore tissue function. Pluripotent stem cells are promising cell candidates for the development of regenerative medicine therapies as they have the ability to selfrenew and commit towards numerous cell types. In vivo, stem cells reside in a dynamic niche, a stem cell-specific microenvironment that possesses chemical, biological and mechanical cues, which drive the stem cell fate and renewal. The connection between stem cells and their niche is a two-way relationship consisting of both cell-cell interaction and cell-extracellular matrix (ECM) interactions. An alternative regenerative medicine approach is the manipulation of the stem cell microenvironment. Hence, novel strategies have been developed including 3D biomaterials and bioreactor technologies providing topographical, chemical and mechanical cues to recreate the stem cell niche. Understanding the mechanisms controlling stem cell fate and the dynamic nature of the stem cell niche will enable researchers to replicate this stem cell-specific microenvironment, and therefore, harness and control the valuable attributes which stem cells possess. This chapter elucidates the importance of pluripotent stem cells and their dynamic niche in regenerative medicine. It further presents novel strategies to replicate chemical, topographical and mechanical stimuli which are essential for the regulation of stem cell fate and hence tissue regeneration.

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“…26 Previous studies have shown that adhesion, migration, and proliferation of MSCs as well as their differentiation into osteoblasts are all strongly dependent on surface topography at the nanoscale. 50, 51 Dalby et al reported that randomly placed nanotopographies were able to induce osteogenic differentiation of MSCs. 52 Osteoblastic differentiation markers, including alkaline phosphatase activity, osteocalcin expression, and mineralization, were measured in the present study to validate the effect of nanoporous alumina on osteogenic differentiation of MSCs in induction medium.…”

Section: Figurementioning

confidence: 99%

In vitro proliferation and osteogenic differentiation of mesenchymal stem cells on nanoporous alumina

Song¹,

Ju²,

Song³

et al. 2013

IJN

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Cell adhesion, migration, and proliferation are significantly affected by the surface topography of the substrates on which the cells are cultured. Alumina is one of the most popular implant materials used in orthopedics, but few data are available concerning the cellular responses of mesenchymal stem cells (MSCs) grown on nanoporous structures. MSCs were cultured on smooth alumina substrates and nanoporous alumina substrates to investigate the interaction between surface topographies of nanoporous alumina and cellular behavior. Nanoporous alumina substrates with pore sizes of 20 nm and 100 nm were used to evaluate the effect of pore size on MSCs as measured by proliferation, morphology, expression of integrin β1, and osteogenic differentiation. An MTT assay was used to measure cell viability of MSCs on different substrates, and determined that cell viability decreased with increasing pore size. Scanning electron microscopy was used to investigate the effect of pore size on cell morphology. Extremely elongated cells and prominent cell membrane protrusions were observed in cells cultured on alumina with the larger pore size. The expression of integrin β1 was enhanced in MSCs cultured on porous alumina, revealing that porous alumina substrates were more favorable for cell growth than smooth alumina substrates. Higher levels of osteoblastic differentiation markers such as alkaline phosphatase, osteocalcin, and mineralization were detected in cells cultured on alumina with 100 nm pores compared with cells cultured on alumina with either 20 nm pores or smooth alumina. This work demonstrates that cellular behavior is affected by variation in pore size, providing new insight into the potential application of this novel biocompatible material for the developing field of tissue engineering.

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Altered nanofeature size dictates stem cell differentiation

Cited by 78 publications

References 38 publications

Control of stem cell fate by engineering their micro and nanoenvironment

Control of stem cell fate by engineering their micro and nanoenvironment

Pluripotent Stem Cells and Their Dynamic Niche

In vitro proliferation and osteogenic differentiation of mesenchymal stem cells on nanoporous alumina

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