Altered Ovarian Regulation of Wound Healing during Aging

Roth, George S.; Harman, S. Mitchell

doi:10.3181/00379727-166-41017

Cited by 11 publications

(11 citation statements)

References 13 publications

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“…According to the studies, there exist a strong correlation between the systemic hormone levels and wound healing. It has been reported that the women at the post-menopausal stage taking systemic hormone replacement therapy heal wounds more effectively than the control ones [5].…”

Section: Q2mentioning

confidence: 99%

Electrospun polyurethane-dextran nanofiber mats loaded with Estradiol for post-menopausal wound dressing

Unnithan

Sasikala

Murugesan

et al. 2015

International Journal of Biological Macromolecules

125

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Section: Q2mentioning

confidence: 99%

Electrospun polyurethane-dextran nanofiber mats loaded with Estradiol for post-menopausal wound dressing

Unnithan

Sasikala

Murugesan

et al. 2015

International Journal of Biological Macromolecules

125

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“…This has been confirmed in clinical studies (9)(10)(11) and in experimental investigations on mice and rats (12-16, t, §). Wound healing rate has even been used as a biological marker ofage in mice.T Previous results suggested that altered estrogen or androgen levels or altered sensitivity to these steroids could account, at least partially, for the slower wound repair in old rats (16). The macrophage has been reported to be an estrogenresponsive cell type.…”

mentioning

confidence: 99%

“…This has been confirmed in clinical studies (9-11) and in experimental investigations on mice and rats (12-16, t, §). Wound healing rate has even been used as a biological marker ofage in mice.T Previous results suggested that altered estrogen or androgen levels or altered sensitivity to these steroids could account, at least partially, for the slower wound repair in old rats (16 clipped and shaved on the back. Four cutaneous wounds were made on each animal by lifting the shaved skin perpendicularly to the length of the mouse and punching twice (once anteriorly on the right side and once posteriorly on the left side) with a leather punch (circumference, 4.7 mm), resulting in two wounds on each side.…”

mentioning

confidence: 99%

Promotion of wound repair in old mice by local injection of macrophages.

Danon

Kowatch

Roth

1989

Proc. Natl. Acad. Sci. U.S.A.

211

138

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Application of peritoneal macrophages to experimentally induced cutaneous wounds of old mice accelerates healing to levels almost comparable to those of untreated young animals. Slightly greater acceleration is observed when macrophages are obtained from young as opposed to old donors. These findings are consistent with a defect in macrophage function as a cause of impaired wound healing in senescence and suggest a possible therapeutic strategy.Macrophages play an important role in wound repair (1) and produce substances that stimulate the synthesis of collagen by fibroblasts (2-4). Furthermore, macrophages produce a substance that causes proliferation of fibroblasts in vitro (5). Activated macrophages have been reported to induce vascular proliferation (6). Although attempts were made to characterize the substances produced by macrophages that enhance wound repair, a simpler way to accelerate the healing process was devised. It consisted of mobilizing the macrophages to the wound area and activating them. The effects of topical application of a variety of substances on the repair process of skin wounds in mice were examined. It was found that promotion of wound repair could be achieved in mice by application of glucan (7). The promotion of wound healing by glucan was evaluated later on single animals, by treating an experimental wound on one rear leg (the wound on the other leg serving as a control) in mice, rats, and guinea pigs. Glucan was found to be effective by accelerating the mobilization of macrophages to the wound (7,8). All these experiments were carried out on young animals or on cells in culture.It is generally accepted that cutaneous wounds heal slower in the elderly as compared with the young. This has been confirmed in clinical studies (9-11) and in experimental investigations on mice and rats (12-16, t, §). Wound healing rate has even been used as a biological marker ofage in mice.T Previous results suggested that altered estrogen or androgen levels or altered sensitivity to these steroids could account, at least partially, for the slower wound repair in old rats (16 clipped and shaved on the back. Four cutaneous wounds were made on each animal by lifting the shaved skin perpendicularly to the length of the mouse and punching twice (once anteriorly on the right side and once posteriorly on the left side) with a leather punch (circumference, 4.7 mm), resulting in two wounds on each side. Wounds were photographed immediately and at subsequent times from a fixed distance with the wound surface perpendicular to the axis of the lens. After development of the film, wound images were projected onto paper at a fixed distance, traced with a pencil, and cut out with sharp scissors. The relative sizes ofthe wounds were assessed by weighing the cut-out paper tracings. Healing was assessed from the mean decrease in wound area for the four wounds on each mouse.Preparation and Application of Peritoneal Macrophages. Mice were anesthetized and 0.5 x 1.0-cm oval incisions were made abdominally. A 20-gau...

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“…8 Furthermore, systemic hormone levels profoundly influence wound healing: post-menopausal females taking systemic hormone replacement therapy heal standardized acute wounds more rapidly than agematched controls, 9 which is in agreement with previous animal studies. 10 The presence of the estrogen receptor in normal skin fibroblasts, as well as in wound fibroblasts and inflammatory cells of both young and aged males and females (Ashcroft G, unpublished observation) suggests that local estrogen levels may influence cutaneous physiology, including the wound healing process. Moreover, it is well-documented that sex hormones modulate immune responses, and an increase in the inflammatory response in specific diseases such as gingivitis can be related to reduced estrogen levels.…”

mentioning

confidence: 99%

Topical Estrogen Accelerates Cutaneous Wound Healing in Aged Humans Associated with an Altered Inflammatory Response

Ashcroft

Greenwell-Wild

Horan

et al. 1999

The American Journal of Pathology

367

304

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The effects of intrinsic aging on the cutaneous wound healing process are profound , and the resulting acute and chronic wound morbidity imposes a substantial burden on health services. We have investigated the effects of topical estrogen on cutaneous wound healing in healthy elderly men and women , and related these effects to the inflammatory response and local elastase levels , an enzyme known to be up-regulated in impaired wound healing states. Eighteen health status-defined females (mean age , 74.4 years) and eighteen males (mean age , 70.7 years) were randomized in a double-blind study to either active estrogen patch or identical placebo patch attached for 24 hours to the upper inner arm , through which two 4-mm punch biopsies were made. The wounds were excised at either day 7 or day 80 post-wounding. Compared to placebo , estrogen treatment increased the extent of wound healing in both males and females with a decrease in wound size at day 7 , increased collagen levels at both days 7 and 80 , and increased day 7 fibronectin levels. In addition , estrogen enhanced the strength of day 80 wounds. Estrogen treatment was associated with a decrease in wound elastase levels secondary to reduced neutrophil numbers , and decreased fibronectin degradation. In vitro studies using isolated human neutrophils indicate that one mechanism underlying the altered inflammatory response involves both a direct inhibition of neutrophil chemotaxis by estrogen and an altered expression of neutrophil adhesion molecules. These data demonstrate that delays in wound healing in the elderly can be significantly diminished by topical estrogen in both male and female subjects. (Am J Pathol 1999, 155:1137-1146) Acute and chronic wound healing morbidity in the elderly imposes a substantial burden on health services.1 Healing of an acute wound involves a reduction in wound size secondary to contraction and re-epithelialization and is accompanied by an increase in collagen deposition.

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Altered Ovarian Regulation of Wound Healing during Aging

Cited by 11 publications

References 13 publications

Electrospun polyurethane-dextran nanofiber mats loaded with Estradiol for post-menopausal wound dressing

Electrospun polyurethane-dextran nanofiber mats loaded with Estradiol for post-menopausal wound dressing

Promotion of wound repair in old mice by local injection of macrophages.

Topical Estrogen Accelerates Cutaneous Wound Healing in Aged Humans Associated with an Altered Inflammatory Response

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